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Depression: 21st Century Solutions + The Dark Side of Wheat

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Abstract Title:

Alpha-glucosidase Inhibitory and Antioxidant Potential of Antidiabetic Herb: Comparative Analyses of Leaf and Callus Solvent Fractions.

Abstract Source:

Pharmacogn Mag. 2016 Oct-Dec;12(48):253-258. PMID: 27867265

Abstract Author(s):

Tsun-Thai Chai, Chee-Siong Khoo, Chong-Siang Tee, Fai-Chu Wong

Article Affiliation:

Tsun-Thai Chai

Abstract:

BACKGROUND: is a medicinal herb which is consumed as vegetable and used as traditional remedies of various ailments in Asia and Africa.

OBJECTIVE: This study aimed to investigate the antiglucosidase and antioxidant activity of solvent fractions ofleaf and callus.

MATERIALS AND METHODS: Leaf and callus methanol extracts were fractionated to produce hexane, chloroform, ethyl acetate, butanol, and water fractions. Antiglucosidase and 1,1-diphenyl-2-picrylhydrazyl scavenging activities as well as total phenolic (TP), total flavonoid (TF), and total coumarin (TC) contents were evaluated. Lineweaver-Burk plot analysis was performed on leaf and callus fractions with the strongest antiglucosidase activity.

RESULTS: Leaf ethyl acetate fraction (LEF) had the strongest antiglucosidase (EC0.55 mg/mL) and radical scavenging (EC10.81μg/mL) activity among leaf fractions. Callus ethyl acetate fraction (CEF) and chloroform fraction had the highest antiglucosidase (EC0.25 mg/mL) and radical scavenging (EC34.12μg/mL) activity, respectively, among callus fractions. LEF and CEF were identified as noncompetitive and competitive α-glucosidase inhibitors, respectively. LEF and CEF had greater antiglucosidase activity than acarbose. Leaf fractions had higher phytochemical contents than callus fractions. LEF had the highest TP, TF, and TC contents. Antiglucosidase and antioxidant activities of leaf fractions correlated with phytochemical contents.

CONCLUSION: LEF had potent antiglucosidase activity and concurrent antioxidant activity. CEF had the highest antiglucosidase activity among all fractions. Callus culture is a promising tool for enhancing production of potentα-glucosidase inhibitors.

SUMMARY: Leaf ethyl acetate fraction (LEF) had the strongest antiglucosidase (EC0.55 mg/mL) and radical scavenging (EC10.81μg/mL) activity among leaf fractionsCallus ethyl acetate fraction (CEF) and chloroform fraction had the highest antiglucosidase (EC0.25 mg/mL) and radical scavenging (EC34.12μg/mL) activity, respectively, among callus fractionsLEF and CEF were identified as noncompetitive and competitive á-glucosidase inhibitors, respectivelyAntiglucosidase and antioxidant activities of leaf fractions correlated with phytochemical contents.LHF: Leaf hexane fraction, LCF: Leaf chloroform fraction, LEF: Leaf ethyl acetate fraction, LBF: Leaf butanol fraction, LWF: Leaf water fraction, CHF: Callus hexane fraction, CCF: Callus chloroform fraction, CEF: Callus ethyl acetate fraction, CBF: Callus butanol fraction, CWF: Callus water fraction, TP: Total phenolic, TF: Total flavonoid, TC: Total coumarin.

Study Type : In Vitro Study

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Sayer Ji
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Depression: 21st Century Solutions + The Dark Side of Wheat

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