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Abstract Title:

Apple polyphenols modulate expression of selected genes related to toxicological defence and stress response in human colon adenoma cells.

Abstract Source:

Int J Cancer. 2008 Jun 15;122(12):2647-55. PMID: 18351577

Abstract Author(s):

Selvaraju Veeriah, Claudia Miene, Nina Habermann, Thomas Hofmann, Stefanie Klenow, Julia Sauer, Frank Böhmer, Stefan Wölfl, Beatrice Louise Pool-Zobel

Abstract:

Apples contain significant amounts of flavonoids that are potentially cancer risk reducing by acting antioxidative or antiproliferative and by favorably modulating gene expression. The purpose of this study was to investigate whether polyphenols from apples modulate expression of genes related to colon cancer prevention in preneoplastic cells derived from colon adenoma (LT97). For this, LT97 cells were treated with effective concentrations of apple extracts (AEs). RNA was isolated and used for synthesis and labeling of cDNA that was hybridized to cDNA-arrays. Gene expression studies were performed using a commercial cDNA-array from Superarray that contains a limited number of genes (96 genes) related to drug metabolism, and a custom-made cDNA microarray that contains a higher number of genes (300 genes, including some genes from Superarray) related to mechanisms of carcinogenesis or chemoprevention. Real-time PCR and enzyme activity assays were additionally performed to confirm selected array results. Treatment of cells with AE resulted in 30 and 46 genes expressed over cut-off values (>or=1.5- or

Study Type : Animal Study

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Sayer Ji
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