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Abstract Title:

An antifungal peptide from the coconut.

Abstract Source:

Peptides. 2005 Dec;26(12):2392-6. PMID: 16308082

Abstract Author(s):

H X Wang, T B Ng

Article Affiliation:

Department of Microbiology, China Agricultural University and State Key Laboratory of Agrobiotechnology, Beijing, China.

Abstract:

A chromatographic procedure consisting of ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and gel filtration by fast performance liquid chromatography on Supedex 75 was utilized to isolate a 10 kDa antifungal peptide from coconut flesh. The peptide was unadsorbed on DEAE-cellulose, but adsorbed on Affi-gel blue gel and CM-cellulose. It displayed antifungal activity against Fusarium oxysporum, Mycosphaerella arachidicola and Physalospora piricola. The IC50 values of its inhibitory activities on mycelial growth in M. arachidicola and HIV-1 reverse transcriptase activity were respectively 1.2 and 52.5 microM.

Study Type : In Vitro Study

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Sayer Ji
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