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Abstract Title:

Anticlastogenic effect of redistilled cow's urine distillate in human peripheral lymphocytes challenged with manganese dioxide and hexavalent chromium.

Abstract Source:

Biomed Environ Sci. 2006 Dec;19(6):487-94. PMID: 17319276

Abstract Author(s):

Dipanwita Dutta, S Saravana Devi, K Krishnamurthi, T Chakrabarti

Article Affiliation:

Environmental Biotechnology Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur--440020, INDIA.

Abstract:

OBJECTIVE: To study the anticlastogenic effect of redistilled cow's urine distillate (RCUD) in human peripheral lymphocytes (HLC) challenged with manganese dioxide and hexavalent chromium. METHODS: The anticlastogenic activity of redistilled cow's urine distillate was studied in human polymorphonuclear leukocytes (HPNLs) and human peripheral lymphocytes in vitro challenged with manganese dioxide and hexavalent chromium as established genotoxicants and clastogens which could cause induction of DNA strand break, chromosomal aberration and micronucleus. Three different levels of RCUD: 1 microL/mL, 50 microL/mL and 100 microL/mL, were used in the study. RESULTS: Manganese dioxide and hexavalent chromium caused statistically significant DNA strand break, chromosomal aberration and micronucleus formation, which could be protected by redistilled cow's urine distillate. CONCLUSION: The redistilled cow's urine distillate posseses strong antigenotoxic and anticlastogenic properties against HPNLs and HLC treated with Cr+6 and MnO2. This property is mainly due to the antioxidants present in RCUD.

Study Type : Animal Study

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Sayer Ji
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Depression: 21st Century Solutions + The Dark Side of Wheat

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