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Depression: 21st Century Solutions + The Dark Side of Wheat

Abstract Title:

Creation of a bacterial cell controlled by a chemically synthesized genome.

Abstract Source:

Science. 2010 Jul 2;329(5987):52-6. Epub 2010 May 20. PMID: 20488990

Abstract Author(s):

Daniel G Gibson, John I Glass, Carole Lartigue, Vladimir N Noskov, Ray-Yuan Chuang, Mikkel A Algire, Gwynedd A Benders, Michael G Montague, Li Ma, Monzia M Moodie, Chuck Merryman, Sanjay Vashee, Radha Krishnakumar, Nacyra Assad-Garcia, Cynthia Andrews-Pfannkoch, Evgeniya A Denisova, Lei Young, Zhi-Qing Qi, Thomas H Segall-Shapiro, Christopher H Calvey, Prashanth P Parmar, Clyde A Hutchison, Hamilton O Smith, J Craig Venter

Article Affiliation:

The J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, MD 20850, USA.

Abstract:

We report the design, synthesis, and assembly of the 1.08-mega-base pair Mycoplasma mycoides JCVI-syn1.0 genome starting from digitized genome sequence information and its transplantation into a M. capricolum recipient cell to create new M. mycoides cells that are controlled only by the synthetic chromosome. The only DNA in the cells is the designed synthetic DNA sequence, including "watermark" sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process. The new cells have expected phenotypic properties and are capable of continuous self-replication.

Study Type : Commentary
Additional Links
Additional Keywords : Mad Science : CK(101) : AC(45)

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Depression: 21st Century Solutions + The Dark Side of Wheat

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