Abstract Title:

Transgenic Peanut (Arachis hypogaea L.) Expressing the Urease Subunit B Gene of Helicobacter pylori.

Abstract Source:

Curr Microbiol. 2011 Aug 11. Epub 2011 Aug 11. PMID: 21833666

Abstract Author(s):

Cheng-Yun Yang, Shuai-Yin Chen, Guang-Cai Duan

Article Affiliation:

School of Basic Medicine, Henan College of Traditional Chinese Medicine, Zhengzhou, 450008, China.

Abstract:

Helicobacter pylori (H. pylori) has been identified as the main pathogenic factors of chronic gastritis and peptic ulcer, and the Class I carcinogen of gastric cancer by WHO. Vaccine has become the most effective measure to prevent and cure H. pylori infection. The UreB is the most effective and common immunogen of all strains of H. pylori and may stimulate the immunoresponse protecting the human body against the challenge of H. pylori. UreB antigen gene was cloned into the binary vector pBI121 which contains a seed-specific promoter Oleosin of peanut and a kanamycin resistance gene, and then UreB gene was transformed into peanut embryo leaflets by Agrobacter-mediated method. The putative transgenic plants were examined for the presence of UreB in the nuclear genome of peanut plants by PCR analysis. Expression of UreB gene in plants was identified by RT-PCR and Western blot analysis. These results suggest that the UreB transgenic peanut can be potentially used as an edible vaccine for controlling H. pylori.

Study Type : In Vitro Study
Additional Links
Additional Keywords : Mad Science : CK(101) : AC(45)

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.