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Abstract Title:

Presence of infectious RD-114 virus in a proportion of canine parvovirus isolates.

Abstract Source:

J Vet Med Sci. 2012 Mar ;74(3):347-50. Epub 2011 Oct 14. PMID: 21997239

Abstract Author(s):

Rokusuke Yoshikawa, Eiji Sato, Takayuki Miyazawa

Article Affiliation:

Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, Kyoto 606–8507, Japan.

Abstract:

We recently found that certain canine live attenuated vaccines produced using `non-feline' cell lines were contaminated with an infectious feline endogenous retrovirus, termed RD-114 virus. We suspected that RD-114 virus may have contaminated the seed stock of canine parvovirus (CPV) during the production of the contaminated vaccines. In this study, we collected stock viruses of CPVs propagated in a feline cell line, and checked the presence of infectious RD-114 virus. Consequently, we found that RD-114 viral RNA was present in all stock viruses, and 7 out of 18 stock viruses were contaminated with infectious RD-114 virus. We also found that RD-114 virus was stable physically and is capable of retaining its infectivity for a long period at -80°C.

Study Type : In Vitro Study

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Sayer Ji
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