Sulforaphane induces cell death in colon cancer cells. - GreenMedInfo Summary

Abstract Title:

Sulforaphane induces cytotoxicity and lysosome- and mitochondria-dependent cell death in colon cancer cells with deleted p53.

Abstract Source:

Toxicol In Vitro. 2011 Oct ;25(7):1302-9. Epub 2011 May 1. PMID: 21557998

Abstract Author(s):

Emil Rudolf, Miroslav Cervinka

Article Affiliation:

Department of Medical Biology and Genetics, Charles University in Prague, Faculty of Medicine in Hradec Kralove, Simkova 870, 500 38 Hradec Kralove, Czech Republic.

Abstract:

Mechanisms and pathways responsible for cytotoxicity of sulforaphane (SF) in colon cancer cells with deleted p53 were investigated during 48h of exposure. SF showed dose-dependent cytotoxicity and proapoptotic activity in the present model. In addition, in HCT-116 p53KO cells SF induced DNA damage with the subsequent cellular response and signaling not including p53 and caspase-2 pathways. Conversely, in SF-treated cells JNK was activated which led to an early lysosomal membrane permeabilization, release of cathepsin B and D and activation of Bid by specific cleavage. Concomitantly, the expression of Bax increased in the presence of JNK-mediated Bcl-2 inhibition which was followed by mitochondrial release of cytochrome c and activation of apoptosis. These results suggest that SF may be useful as a chemopreventive agent in colon cancer with inactivated or lost p53.

Pubmed Data : Toxicol In Vitro. 2011 Oct ;25(7):1302-9. Epub 2011 May 1. PMID: 21557998
Article Published Date : Oct 01, 2011
Study Type : In Vitro Study
Additional Links
Substances : Sulforaphane : CK(484) : AC(167)
Diseases : Colon Cancer : CK(819) : AC(233)
Pharmacological Actions : Apoptotic : CK(1416) : AC(1089)

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