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Abstract Title:

Induction of G₂/M arrest and apoptosis by sulforaphane in human osteosarcoma U2-OS cells.

Abstract Source:

Mol Med Report. 2011 Sep-Oct;4(5):929-34. Epub 2011 Jun 29. PMID: 21720717

Abstract Author(s):

Mi-Ran Kim, Lu Zhou, Byung-Hyun Park, Jung Ryul Kim

Article Affiliation:

Department of Orthopaedic Surgery, Medical School, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.

Abstract:

Sulforaphane is one of the most abundant isothiocyanates found in certain cruciferous vegetables, particularly broccoli. To date, sulforaphane has gained attention as a chemopreventive compound. The mechanism responsible for the anticancer effects of sulforaphane in osteosarcoma, however, is not clear. In this study, we demonstrate an anti-proliferative mechanism of sulforaphane in human osteosarcoma cells. The treatment of cells with sulforaphane resulted in a concentration- and time‑dependent inhibition of growth and G2/M phase arrest of the cell cycle. This effect was associated with a decrease in protein expression of cyclin A and B1 and their activating partners, cyclin-dependent kinases (CDKs) 1 and 2, with concomitant up-regulation of p21, a CDK inhibitor. Sulforaphanetreatment also resulted in apoptosis as evidenced by an increase in annexin V+/propidium iodide- (V+/PI-) cells, the cleavage of 116-kDa poly (ADP-ribose) polymerase (PARP) and ICAD and oligonucleosomal DNA fragmentation. Taken together, these findings indicate that the molecular mechanisms underlying sulforaphane-mediated growth inhibition in U2-OS cells may be the modulation of the cell cycle machinery and the induction of apoptosis.

Study Type : In Vitro Study

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Sayer Ji
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