Synergistic actions of vitamin D and metformin on skeletal muscles and insulin resistance of type 2 diabetic rats. - GreenMedInfo Summary
Synergistic actions of Vitamin D and metformin on skeletal muscles and insulin resistance of Type 2 Diabetic Rats.
J Cell Physiol. 2017 Dec 2. Epub 2017 Dec 2. PMID: 29205344
Shaimaa N Amin
Diabetes Mellitus is a chronic generalized disorder due to insulin insufficiency or resistance. Skeletal muscles represent one of the most important target organs that is affected by insulin signaling. The aim of the current work was to investigate the effect of metformin versus vitamin D (and also simultaneous administration) therapy in type 2 diabetic (T2D) rats on the state of the muscle and insulin sensitivity. Thirty six male rats constituted the animal model and have been divided into five groups: control, Diabetic, Diabetic+Metformin, Diabetic+ VitaminD, Diabetic+Metformin+Vitamin D. blood samples were taken for biochemical measurements of serum calcium, interleukin-6 (IL-6), Triglycerides (TG), glucose, insulin, and calculation of HOMA-IR, and then rats were sacrificed , dissected for removal of gastrocnemius muscle that is used for both biochemical, histopathological and electron microscopy examination. Oral administration of vitamin D alone or in combination with metformin improved insulin sensitivity in skeletal muscles, and sustained the metabolic complications along with muscle atrophy and inflammation in T2D rats. We demonstrated super-beneficial action on insulin resistance of additional vitamin D therapy in T2DM rats that were insufficiently controlled by metformin alone. This article is protected by copyright. All rights reserved.