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Depression: 21st Century Solutions + The Dark Side of Wheat

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Abstract Title:

Thymoquinone induces apoptosis in B16-F10 melanoma cell through inhibition of p-STAT3 and inhibits tumor growth in a murine intracerebral melanoma model.

Abstract Source:

World Neurosurg. 2018 Mar 3. Epub 2018 Mar 3. PMID: 29510292

Abstract Author(s):

Mustafa Aziz Hatiboglu, Abdurrahim Kocyigit, Eray Metin Guler, Kerime Akdur, Arife Nalli, Ersin Karatas, Saffet Tuzgen

Article Affiliation:

Mustafa Aziz Hatiboglu

Abstract:

BACKGROUND: Prognosis of patients with melanoma brain metastasis is poor despite various chemotherapeutic agents. Researchers focus on finding effective treatment with low risk of toxicity. Thymoquinone (TQ) has been found to be effective on different types of cancer. However, no data exists on the effect of TQ in intracerebral melanoma. The purpose of this study was to assess the effect of TQ in B16-F10 melanoma cell in vitro and intracerebral melanoma in vivo.

METHODS: The mechanisms of efficacy were investigated by ATP assay for cytotoxicity, flow cytometry and Acridine-orange staining for apoptosis, Comet assay for genotoxicity, CM-HDCF-DA (2,7-dichlorodihydrofluorescein) for intracellular reactive oxygen species (ROS) generation and ELISA methods for inflammatory cytokines. Western blotting was performed to assess the expressions of p-JAK2, p-STAT3, caspase-3, bax, Bcl-2, and survivin. Also, the effect of TQ was investigated in a model system of intracerebral melanoma in syngeneic mice.

RESULTS: The median survival was improved by TQ in mice with intracerebral melanoma compared to the control group (16 days vs. 9 days, p=0.008). Cytotoxicity was enhanced by TQ in B16-F10 cells in a dose-dependent manner. TQ also induced apoptosis, DNA damage and increase in intracellular ROS. TQ inhibited p-STAT3, resulting in apoptosis through regulation of pro- and anti-apoptotic proteins.

CONCLUSIONS: Our findings suggest that TQ would be an effective treatment in intracerebral metastatic lesions. This warrants further investigation.

Study Type : In Vitro Study

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Sayer Ji
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Depression: 21st Century Solutions + The Dark Side of Wheat

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