n/a
Article Publish Status: FREE
Abstract Title:

Withania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's).

Abstract Source:

BMC Complement Altern Med. 2018 Apr 10 ;18(1):126. Epub 2018 Apr 10. PMID: 29631586

Abstract Author(s):

Dhaneshree Bestinee Naidoo, Anil Amichund Chuturgoon, Alisa Phulukdaree, Kanive Parashiva Guruprasad, Kapaettu Satyamoorthy, Vikash Sewram

Article Affiliation:

Dhaneshree Bestinee Naidoo

Abstract:

BACKGROUND: Cancer and inflammation are associated with cachexia. Withania somnifera (W. somnifera) possesses antioxidant and anti-inflammatory potential. We investigated the potential of an aqueous extract of the root of W. somnifera (W) to modulate cytokines, antioxidants and apoptosis in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's).

METHODS: Cytotoxcity of Wwas determined at 24 and 72 h (h). Oxidant scavenging activity of Wwas evaluated (2, 2-diphenyl-1 picrylhydrazyl assay). Glutathione (GSH) levels, caspase (- 8, - 9, - 3/7) activities and adenosine triphosphate (ATP) levels (Luminometry) were thereafter assayed. Tumour necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β and IL-10 levels were also assessed using enzyme-linked immunosorbant assay.

RESULTS: At 24 h, W(0.2-0.4 mg/ml) decreased PBMC viability between 20 and 25%, whereas it increased THP-1 viability between 15 and 23% (p < 0.001). At 72 h, Wincreased PBMC viability by 27-39% (0.05, 0.4 mg/ml W) whereas decreased THP-1 viability between 9 and 16% (0.05-0.4 mg/ml W) (p < 0.001). Oxidant scavenging activity was increased by W(0.05-0.4 mg/ml, p < 0.0001). PBMC TNF-α and IL-10 levels were decreased by 0.2-0.4 mg/ml Wwhereas IL-1β levels were increased by 0.05-0.4 mg/ml W(p < 0.0001). In THP-1 cells, W(0.05-0.4 mg/ml) decreased TNF-α, IL-1β and IL-6 levels (p < 0.0001). At 24 h, GSH levels were decreased in PBMC's, whilst increased in THP-1 cells by 0.2-0.4 mg/ml W(p < 0.0001). At 72 h, W(0.1-0.4 mg/ml) decreased GSH levels in both cell lines (p < 0.0001). At 24 h, W(0.2-0.4 mg/ml) increased PBMC caspase (-8, -3/7) activities whereas W(0.05, 0.1, 0.4 mg/ml) increased THP-1 caspase (-9, -3/7) activities (p < 0.0001). At 72 h, PBMC caspase (-8, -9, -3/7) activities were increased at 0.05-0.1 mg/ml W(p < 0.0001). In THP-1 cells, caspase (-8, -9, -3/7) activities and ATP levels were increased by 0.1-0.2 mg/ml Wwhereas decreased by 0.05 and 0.4 mg/ml W(72 h, p < 0.0001).

CONCLUSION: In PBMC's and THP-1 cells, Wproved to effectively modulate antioxidant activity, inflammatory cytokines and cell death. In THP-1 cells, Wdecreased pro-inflammatory cytokine levels, which may alleviate cancer cachexia and excessive leukaemic cell growth.

Print Options


Key Research Topics

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2024 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.