Cancers: Canine https://greenmedinfo.com/taxonomy/term/12920/all en A mixed grape and blueberry extract is safe for dogs to consume. https://greenmedinfo.com/article/mixed-grape-and-blueberry-extract-safe-dogs-consume PMID:  BMC Vet Res. 2016 ;12(1):162. Epub 2016 Aug 3. PMID: 27487916 Abstract Title:  A mixed grape and blueberry extract is safe for dogs to consume. Abstract:  BACKGROUND: Grape and blueberry extracts are known to protect against age-related cognitive decline. However, beneficial effects achieved by mixing grape and blueberry extracts have yet to be evaluated in dogs, or their bioavailability assessed. Of concern to us were cases of acute renal failure in dogs, after their ingestion of grapes or raisins. The European Pet Food Industry Federation (2013) considers only the grape or raisin itself to be potentially dangerous; grape-seed extracts per-se, are not considered to be a threat. Our aim was therefore to evaluate the renal and hepatic safety, and measure plasma derivatives of a polyphenol-rich extract from grape and blueberry (PEGB; from the Neurophenols Consortium) in dogs. Polyphenol expression was analyzed by UHPLC-MS/MS over 8 hours, for dogs given PEGB at 4 mg/kg. Safety was evaluated using four groups of 6 dogs. These groups received capsules containing no PEGB (control), or PEGB at 4, 20, or 40 mg/kg BW/d, for 24 weeks. Blood and urine samples were taken the week prior to study commencement, then at the end of the24-wk study period. Routine markers of renal and liver damage, including creatinine (Creat), blood urea nitrogen, albumin, minerals, alkaline phosphatase (ALP), and alanine transaminase (ALT) were measured. Biomarkers for early renal damage were also evaluated in plasma (cystatin C (CysC), and neutrophil gelatinase-associated lipocalin (NGAL)), and urine (CysC, clusterin (Clu), and NGAL). Ratios of urinary biomarkers to Creat were calculated, and compared with acceptable maximal values obtained for healthy dogs, as reported in the literature.RESULTS: While several PEGB-specific polyphenols and metabolites were detected in dog plasma, at the end of the PEGB consumption period, our biomarker analyses presented no evidence of either renal or liver damage (Creat, BUN, ionogram, albumin and ALT, ALP). Similarly, no indication of early renal damage could be detected. Plasma CysC, urinary CysC/Creat, Clu/Creat, and NGAL/Creat ratios were all beneath reported benchmarked maximums, with no evidence of PEGB toxicity.CONCLUSIONS: Long-term consumption of a pet specific blend of a polyphenol-rich extract from grape and blueberry (PEGB; from the Neurophenols Consortium), was not associated with renal or hepatic injury, and can therefore be considered safe. https://greenmedinfo.com/article/mixed-grape-and-blueberry-extract-safe-dogs-consume#comments Blueberry Cancers: Canine Grape Seed Extract Plant Extracts Animal Study Fri, 19 Aug 2016 17:13:13 +0000 greenmedinfo 133252 at https://greenmedinfo.com An extract of potato inhibits a variety of cancer cell lines, as well as Herpes simplex. https://greenmedinfo.com/article/extract-potato-inhibits-variety-cancer-cell-lines-well-herpes-simplex PMID:  Fitoterapia. 2010 Sep;81(6):600-6. Epub 2010 Mar 20. PMID: 20227470 Abstract Title:  Regeneration, in vitro glycoalkaloids production and evaluation of bioactivity of callus methanolic extract of Solanum tuberosum L. Abstract:  Callus and differentiated shoots initiated from Solanum tuberosum L. on MS media containing BA, IAA, and Kin. Glycoalkaloids are produced in callus and shoots in concentrations higher than original tubers using HPLC. Callus methanolic extract had promising anticancer activity with low IC(50) values against human carcinoma cell lines of breast, lymphoplastic leukemia, larynx, liver, cervix, colon, and brain, IC(50) (microg/mL) were 2.7, 3.7, 6, 6.7, 10, 13.6, and 22.3 respectively. Antioxidant capacity of the extract (76.4%) performed using ESR. Preliminary screening showed that the extract exhibited in vitro virucidal activity against Herpes simplex. The extract possessed in vitro schistomicidal and fasciolicidal activity. https://greenmedinfo.com/article/extract-potato-inhibits-variety-cancer-cell-lines-well-herpes-simplex#comments Brain Cancer Breast Cancer Cancers: Canine Cervical Cancer Colon Cancer Herpes family viruses Laryngeal Cancer Leukemia Liver Cancer Potato Antiproliferative Antiviral Agents In Vitro Study Sat, 02 Oct 2010 19:09:25 +0000 greenmedinfo 57274 at https://greenmedinfo.com Benzyl isothiocyanate induces apoptosis and inhibits tumor growth in canine mammary carcinoma. https://greenmedinfo.com/article/benzyl-isothiocyanate-induces-apoptosis-and-inhibits-tumor-growth-canine-mamma PMID:  Front Vet Sci. 2020 ;7:580530. Epub 2020 Nov 11. PMID: 33263014 Abstract Title:  Benzyl Isothiocyanate Induces Apoptosis and Inhibits Tumor Growth in Canine Mammary Carcinoma via Downregulation of the Cyclin B1/Cdk1 Pathway. Abstract:  Canine mammary carcinoma is common in female dogs, and its poor prognosis remains a serious clinical challenge, especially in developing countries. Benzyl isothiocyanate (BITC) has attracted great interest because of its inhibitory effect against tumor activity. However, its effect and the underlying mechanisms of action in canine mammary cancer are not well-understood. Here, we show that BITC suppresses mammary tumor growth, bothand, and reveal some of the potential mechanisms involved.The effect of BITC on canine mammary cancer was evaluated on CIPp and CMT-7364, canine mammary carcinoma lines. The cell lines were treated with BITC and then subjected to wound healing and invasion assays. Cell cycles and apoptosis were measured using flow cytometry; TUNEL assay; immunohistochemistry (IHC) for caspase 3, caspase 9, and cyclin D1; hematoxylin and eosin (H&amp;E) staining; and/or quantitative polymerase chain reaction (qPCR).BITC showed a strong suppressive effect in both CIPp and CMT-7364 cells by inhibiting cell growth; these effects were both dose- and time-dependent. BITC also inhibited migration and invasion of CIPp and CMT-7364 cells. BITC induced G2 arrest and apoptosis, decreasing tumor growth in nude mice by downregulation of cyclin B1 and Cdk1 expression.BITC suppressed both invasion and migration of CIPp and CMT-7364 cells and induced apoptosis. BITC inhibited canine mammary tumor growth by suppressing cyclinB1 and Cdk1 expression in nude mice. <p><a href="https://greenmedinfo.com/article/benzyl-isothiocyanate-induces-apoptosis-and-inhibits-tumor-growth-canine-mamma" target="_blank">read more</a></p> https://greenmedinfo.com/article/benzyl-isothiocyanate-induces-apoptosis-and-inhibits-tumor-growth-canine-mamma#comments Benzyl isothiocyanate Cancers: Canine Anti-metastatic Antiproliferative Apoptotic Cell cycle arrest Animal Study Tue, 06 Apr 2021 19:01:08 +0000 greenmedinfo 237526 at https://greenmedinfo.com Betulinic acid has anti-proliferative effects on canine cancers. https://greenmedinfo.com/article/betulinic-acid-has-anti-proliferative-effects-canine-cancers n/a PMID:  In Vivo. 2018 Sep-Oct;32(5):1081-1088. PMID: 30150429 Abstract Title:  Antitumor Activity of Betulinic Acid and Betulin in Canine Cancer Cell Lines. Abstract:  BACKGROUND/AIM: Betulinic acid (BA) and betulin (BT) exhibit a variety of pharmacological properties including anti-cancer, anti-inflammatory and anti-oxidant ones. Canine lymphoma and osteosarcoma have a high mortality rate and need more effective therapeutic approaches. In this study, the anti-proliferative and pro-apoptotic effects of BA and BT were investigated in canine T-cell lymphoma (CL-1), canine B-cell lymphoma (CLBL-1) and canine osteosarcoma (D-17) cell lines. MATERIALS AND METHODS: The cultured cells were treated with several concentrations of BA or BT for 24, 48 and 72 h, and cell proliferation was assessed by the MTT assay. Cell apoptotic rate and cell cycle were analyzed using flow cytometry. RESULTS: Anti-proliferative effect of BT and BA was concentration- and time-dependent. Moreover, BA and BT arrested cell cycle in S phase in CL-1 and D-17 cells, and in G/Gphase in CLBL-1 cells. CONCLUSION: Both compounds showed an antitumor activity, and the effects of BA were stronger than that of BT. https://greenmedinfo.com/article/betulinic-acid-has-anti-proliferative-effects-canine-cancers#comments Betulinic acid Cancers: Canine Antineoplastic Agents Antiproliferative Apoptotic Cell cycle arrest In Vitro Study Sat, 06 Apr 2019 00:01:48 +0000 greenmedinfo 184378 at https://greenmedinfo.com BPA might facilitate the progression of prostate tumorigenesis in beagle dogs via cfa-miR-204. https://greenmedinfo.com/article/bpa-might-facilitate-progression-prostate-tumorigenesis-beagle-dogs-cfa-mir-20 PMID:  Ecotoxicol Environ Saf. 2022 Mar 21 ;235:113430. Epub 2022 Mar 21. PMID: 35325610 Abstract Title:  Bisphenol A exposure triggers the malignant transformation of prostatic hyperplasia in beagle dogs via cfa-miR-204/KRAS axis. Abstract:  The prostatic toxicity of bisphenol A (BPA) exposure is mainly associated with hormonal disturbances, thus interfering with multiple signal pathways and increasing the susceptibility to prostatic lesions. This study concentrates predominantly on the potential effect and mechanisms of low-dose BPA exposure on prostates in adult beagle dogs. The dogs were orally given BPA (2, 6, 18 μg/kg/day) and vehicle for 8 weeks, followed by blood collection and dissection. The ascended organ coefficient and volume of prostates, thickened epithelium, as well as histopathological observation have manifested that BPA exposure could trigger the aberrant prostatic hyperplasia in beagle dogs. Hormone level detection revealed that the ratios of estradiol (E) to testosterone (T) (E/T) and prolactin (PRL) to T (PRL/T) were up-regulated in the serum from BPA group. Based on microRNA (miRNA) microarray screening and functional enrichment analysis, BPA might facilitate the progression of prostate tumorigenesis in beagle dogs via cfa-miR-204 and its downstream target KRAS oncogene. Subsequently, the overexpression of KRAS, CDKN1A, MAPK1, VEGFA, BCL2 and PTGS2 was validated. These findings provide a series of underlying targets for preventing the initiation and metastasis of BPA-induced prostatic hyperplasia and tumorigenesis, while the regulatory relationship headed with KRAS requires further investigation. <p><a href="https://greenmedinfo.com/article/bpa-might-facilitate-progression-prostate-tumorigenesis-beagle-dogs-cfa-mir-20" target="_blank">read more</a></p> https://greenmedinfo.com/article/bpa-might-facilitate-progression-prostate-tumorigenesis-beagle-dogs-cfa-mir-20#comments Bisphenol Toxicity Cancers: Canine Bisphenol A Animal Study Sat, 02 Apr 2022 00:59:48 +0000 greenmedinfo 255607 at https://greenmedinfo.com Cannabidiol induces apoptosis and perturbs mitochondrial function in human and canine glioma cells. https://greenmedinfo.com/article/cannabidiol-induces-apoptosis-and-perturbs-mitochondrial-function-human-and-ca PMID:  Front Pharmacol. 2021 ;12:725136. Epub 2021 Aug 11. PMID: 34456736 Abstract Title:  Cannabidiol Induces Apoptosis and Perturbs Mitochondrial Function in Human and Canine Glioma Cells. Abstract:  Cannabidiol (CBD), the major non-psychoactive compound found in cannabis, is frequently used both as a nutraceutical and therapeutic. Despite anecdotal evidence as an anticancer agent, little is known about the effect CBD has on cancer cells. Given the intractability and poor prognoses of brain cancers in human and veterinary medicine, we sought to characterize thecytotoxicity of CBD on human and canine gliomas. Glioma cells treated with CBD showed a range of cytotoxicity from 4.9 to 8.2 μg/ml; canine cells appeared to be more sensitive than human. Treatment with&gt;5 μg/ml CBD invariably produced large cytosolic vesicles. The mode of cell death was then interrogated using pharmacologic inhibitors. Inhibition of apoptosis was sufficient to rescue CBD-mediated cytotoxicity. Inhibition of RIPK3, a classical necroptosis kinase, also rescued cells from death and prevented the formation of the large cytosolic vesicles. Next, cellular mitochondrial activity in the presence of CBD was assessed and within 2 hours of treatment CBD reduced oxygen consumption in a dose dependent manner with almost complete ablation of activity at 10 μg/ml CBD. Fluorescent imaging with a mitochondrial-specific dye revealed that the large cytosolic vesicles were, in fact, swollen mitochondria. Lastly, calcium channels were pharmacologically inhibited and the effect on cell death was determined. Inhibition of mitochondrial channel VDAC1, but not the TRPV1 channel, rescued cells from CBD-mediated cytotoxicity. These results demonstrate the cytotoxic nature of CBD in human and canine glioma cells and suggest a mechanism of action involving dysregulation of calcium homeostasis and mitochondrial activity. <p><a href="https://greenmedinfo.com/article/cannabidiol-induces-apoptosis-and-perturbs-mitochondrial-function-human-and-ca" target="_blank">read more</a></p> https://greenmedinfo.com/article/cannabidiol-induces-apoptosis-and-perturbs-mitochondrial-function-human-and-ca#comments Cancers: Canine Cannabidiol Apoptotic Animal Study Wed, 06 Oct 2021 00:15:45 +0000 greenmedinfo 246845 at https://greenmedinfo.com Celastrol inhibits canine mammary tumor cells by inducing apoptosis via the caspase pathway. https://greenmedinfo.com/article/celastrol-inhibits-canine-mammary-tumor-cells-inducing-apoptosis-caspase-pathw PMID:  Front Vet Sci. 2021 ;8:801407. Epub 2022 Feb 4. PMID: 35187141 Abstract Title:  Celastrol Inhibits Canine Mammary Tumor Cells by Inducing Apoptosisthe Caspase Pathway. Abstract:  Canine mammary tumor is a serious disease threatening the health of dogs and can be used as a research model for human breast cancer. The study of canine mammary tumor has a role in improving the welfare of dogs. Most common canine mammary tumor chemotherapy drugs have limited effects and drug resistance. Celastrol is an extract of, which has a wide range of biological activities, including significant anti-tumor effects. At present, celastrol has not been used in the clinical treatment for canine mammary tumor. This study investigated the anti-tumor properties of celastrol throughassay of cell proliferation inhibition, cell colony, cell migration, and invasion; flow cytometry, qPCR, and Western Blot methods were used to explore the anti-tumor mechanism of celastrol. The results showed that celastrol can inhibit the proliferation of canine mammary tumor cells, and decrease the migration and invasion ability of canine mammary tumor cells. We also found that celastrol can upregulate Cleaved Caspase-3 and Cleaved Caspase-9 protein expression levels to promote cell apoptosis, and can regulate cell cycle-related proteins to induce cell cycle arrest. In summary, celastrol may inhibit canine mammary tumor cells through the Caspase pathway, providing a new direction for anti-canine mammary tumor drugs, and is expected to become a new anti-cancer drug for canine mammary tumors. <p><a href="https://greenmedinfo.com/article/celastrol-inhibits-canine-mammary-tumor-cells-inducing-apoptosis-caspase-pathw" target="_blank">read more</a></p> https://greenmedinfo.com/article/celastrol-inhibits-canine-mammary-tumor-cells-inducing-apoptosis-caspase-pathw#comments Cancers: Canine Celastrol Antiproliferative Apoptotic In Vitro Study Sun, 03 Apr 2022 18:33:13 +0000 greenmedinfo 255714 at https://greenmedinfo.com Chemo and Radiation Can Make Cancer More Malignant https://greenmedinfo.com/blog/does-chemo-radiation-actually-make-cancer-more-malignant <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2018<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="Chemo and Radiation Actually Make Cancer More Malignant" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/sayerji/images/k-age.jpg" style="width: 400px; height: 259px;" /></p> <p><span style="font-size:22px;"><em><strong>Cancer is the second leading cause of death in the developed world, and yet we are still in the dark ages when it comes to treating and understanding it.</strong></em></span></p><p><a href="https://greenmedinfo.com/blog/does-chemo-radiation-actually-make-cancer-more-malignant" target="_blank">read more</a></p> https://greenmedinfo.com/blog/does-chemo-radiation-actually-make-cancer-more-malignant#comments All-Cause Mortality Auricular Cancer Bile Duct Cancer Bladder Cancer Breast Cancer Cancer Patients Cancers: All Cancers: Canine Cancers: Drug Resistant Cancers: Radiation-Induced Colon Cancer Lung Cancer Cancer Health Guide: Breast Cancer Tue, 31 Jul 2012 16:35:52 +0000 Sayer Ji 73384 at https://greenmedinfo.com European mistletoe extract is cytotoxic to canine high-grade astrocytoma cells. https://greenmedinfo.com/article/european-mistletoe-extract-cytotoxic-canine-high-grade-astrocytoma-cells PMID:  Vet Sci. 2022 Jan 15 ;9(1). Epub 2022 Jan 15. PMID: 35051115 Abstract Title:  European Mistletoe () Extract Is Cytotoxic to Canine High-Grade Astrocytoma Cells In Vitro and Has Additive Effects with Mebendazole. Abstract:  Malignant gliomas are associated with extremely poor clinical outcomes in both humans and dogs, and novel therapies are needed. Glioma-bearing canine patients may serve as promising preclinical models for human therapies, including complementary medicine. The objective of this study was to evaluate the effects of mistletoe extract () alone and in combination with mebendazole in an in vitro model of canine high-grade astrocytoma using the cell line SDT-3G. SDT-3G cells were exposed to a range of concentrations of mistletoe extract alone to obtain an IC50. In separate experiments, cells were exposed to mebendazole at a previously determined IC50 (0.03µM) alone or in conjunction with varying concentrations of mistletoe extract to determine the additive effects. The IC50 for mistletoe alone was 5.644 ± 0.09 SD μg/mL. The addition of mistletoe at 5 μg/mL to mebendazole at 0.03 µM led to increased cell death compared to what would be expected for each drug separately. The cytotoxicity of mistletoe in vitro and its additive effect with mebendazole support future expanded in vitro and in vivo studies in dogs and supply early evidence that this may be a useful adjunct therapeutic agent for use in glioma-bearing dogs. To the authors&#039; knowledge, this is the first published report ofextract in canine glioma. <p><a href="https://greenmedinfo.com/article/european-mistletoe-extract-cytotoxic-canine-high-grade-astrocytoma-cells" target="_blank">read more</a></p> https://greenmedinfo.com/article/european-mistletoe-extract-cytotoxic-canine-high-grade-astrocytoma-cells#comments Cancers: Canine Mistletoe Cytotoxic In Vitro Study Wed, 29 Jun 2022 00:36:57 +0000 greenmedinfo 259807 at https://greenmedinfo.com Fucoxanthin exerts anti-tumor activity on canine mammary tumor cells. https://greenmedinfo.com/article/fucoxanthin-exerts-anti-tumor-activity-canine-mammary-tumor-cells PMID:  Animals (Basel). 2021 May 23 ;11(6). Epub 2021 May 23. PMID: 34071074 Abstract Title:  Fucoxanthin Exerts Anti-Tumor Activity on Canine Mammary Tumor Cells via Tumor Cell Apoptosis Induction and Angiogenesis Inhibition. Abstract:  Fucoxanthin is a carotenoid derived from brown algae. It is known to exhibit anticancer activity, including the promotion of apoptosis and cell cycle arrest in several tumors. However, it remains unclear whether fucoxanthin exhibits anticancer activity against mammary gland tumors. In this study, we evaluated fucoxanthin activity against canine mammary tumor cells (CMT-U27) and human umbilical vein endothelial cells (HUVECs) to investigate its effect on cell viability, migration, tube formation, and angiopoietin 2 (Ang2) expression. Our results showed that fucoxanthin induced apoptosis via caspase activation in CMT-U27 cells. In rat aortic ring assay, fucoxanthin suppressed endothelial cell sprouting. Furthermore, fucoxanthin inhibited tube formation and migration in HUVECs. The number of migrated cells was assessed using CMT-U27 cells. The results demonstrated that fucoxanthin exerted anti-angiogenic activity on HUVECs and CMT-U27 cells by promoting Ang2 expression. In conclusion, our results demonstrated that fucoxanthin induced tumor cell death and inhibited angiogenesis, suggesting that fucoxanthin could be considered as a promising therapeutic agent for canine mammary gland tumors. <p><a href="https://greenmedinfo.com/article/fucoxanthin-exerts-anti-tumor-activity-canine-mammary-tumor-cells" target="_blank">read more</a></p> https://greenmedinfo.com/article/fucoxanthin-exerts-anti-tumor-activity-canine-mammary-tumor-cells#comments Cancers: Canine Fucoxanthin Angiogenesis Inhibitors Apoptotic In Vitro Study Sun, 06 Jun 2021 22:26:57 +0000 greenmedinfo 240836 at https://greenmedinfo.com Luteolin induced cell death by initiating mitochondrial dysfunction and regulating intracellular signal transduction in canine osteosarcoma cells. https://greenmedinfo.com/article/luteolin-induced-cell-death-initiating-mitochondrial-dysfunction-and-regulatin PMID:  J Cell Physiol. 2018 Oct 26. Epub 2018 Oct 26. PMID: 30362587 Abstract Title:  Effects of luteolin on canine osteosarcoma: Suppression of cell proliferation and synergy with cisplatin. Abstract:  Canine osteosarcoma is characterized by aggressiveness, easy metastasis to the lungs, and high mortality after standard therapy. Luteolin is a flavonoid found in vegetables and fruits and has diverse functions. Elucidation of the biological mechanisms of luteolin on canine osteosarcoma will enhance the efficacy of chemotherapeutic agents in canine tumors. In this study, we examined the effects of luteolin in the canine osteosarcoma cell lines, D17 and DSN. The results of this study show that luteolin inhibited canine osteosarcoma cell proliferation and induced apoptosis by altering cell-cycle proportion, producing reactive oxygen species, increasing the loss of mitochondrial membrane potential, and reducing cytosolic Caconcentration. In addition, luteolin activated ERK1/2 and inactivated phosphoinositide 3-kinase/AKT signaling in canine osteosarcoma cells. Moreover, luteolin showed synergistic effects with cisplatin to reduce cell proliferation. In summary, luteolin induced cell death by initiating mitochondrial dysfunction and regulating intracellular signal transduction in canine osteosarcoma cells. <p><a href="https://greenmedinfo.com/article/luteolin-induced-cell-death-initiating-mitochondrial-dysfunction-and-regulatin" target="_blank">read more</a></p> https://greenmedinfo.com/article/luteolin-induced-cell-death-initiating-mitochondrial-dysfunction-and-regulatin#comments Cancers: Canine Luteolin Antiproliferative Apoptotic In Vitro Study Tue, 06 Nov 2018 16:38:09 +0000 greenmedinfo 173504 at https://greenmedinfo.com Maitake D-fraction has potential growth inhibitory effects in canine cancer cells. https://greenmedinfo.com/article/maitake-d-fraction-has-potential-growth-inhibitory-effects-canine-cancer-cells PMID:  Vet Ther. 2004;5(4):263-71. PMID: 15719326 Abstract Title:  Potential growth inhibitory effect of maitake D-fraction on canine cancer cells. Abstract:  The postulated anticancer effect of D-fraction, the bioactive extract of maitake mushroom, on three types (CF33, CF21, and CL-1) of canine cancer cells was evaluated. The effect of D-fraction on several human cancer cells was also investigated. The effect of other beta-glucan products was likewise examined. D-fraction was highly effective on the canine cancer cells, either potently inhibiting cell growth or directly killing cells. Similar effects were also demonstrated in certain human cancer cells. However, other beta-glucan products relevant to D-fraction had no such effects on canine cancer cells. Therefore, D-fraction is a potent natural agent that could be useful in treating canine cancers as well as other veterinary cancers. https://greenmedinfo.com/article/maitake-d-fraction-has-potential-growth-inhibitory-effects-canine-cancer-cells#comments Cancers: Canine Maitake Mushroom In Vitro Study Fri, 15 Jan 2010 01:23:50 +0000 greenmedinfo 49233 at https://greenmedinfo.com Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells. https://greenmedinfo.com/article/positive-effects-antitumor-drugs-combination-propolis-canine-osteosarcoma-cell PMID:  Biomed Pharmacother. 2018 May 15 ;104:268-274. Epub 2018 May 15. PMID: 29775894 Abstract Title:  Positive effects of antitumor drugs in combination with propolis on canine osteosarcoma cells (spOS-2) and mesenchymal stem cells. Abstract:  The combination of lower concentrations of antitumor drugs (carboplatin - CARB, doxorubicin - DOX, and methotrexate - MET) with propolis was investigated against canine osteosarcoma (spOS-2) and mesenchymal stem cells (MSC) in vitro. The mechanism of action in the combinations was analyzed. spOS-2 cells were incubated up to 72 h with propolis (50 μg/ml) alone or in combination with CARB (10-400 μmol/l), DOX (0.5-2 μmol/l) or MET (50-200 μmol/l). Cell viability was assessed by MTT assay, apoptosis/necrosis by flow cytometry, and MSC was incubated with the optimum combination. Propolis alone exerted no cytotoxic action against spOS-2 cells, whereas CARB (400, 200 and 100 μmol/l) exhibited the highest cytotoxic effects comparing to DOX and MET. The combination of propolis with the lowest concentrations of CARB led to better results comparing to CARB alone, which was not observed using DOX and MET. Apoptosis was involved in the action of propolis + CARB in spOS-2 cells. MSC were not affected by CARB/propolis, indicating that the cytotoxic action of the combination was specific to tumor cells but not to normal ones. Propolis improved the action of CARB against spOS-2 cells using lower concentrations of this drug, without affecting MSC. These findings are relevant and indicate a possible application of propolis in OSA treatment. <p><a href="https://greenmedinfo.com/article/positive-effects-antitumor-drugs-combination-propolis-canine-osteosarcoma-cell" target="_blank">read more</a></p> https://greenmedinfo.com/article/positive-effects-antitumor-drugs-combination-propolis-canine-osteosarcoma-cell#comments Animal Cancers Cancers: Canine Propolis: Bee Cytotoxic Natural Substance/Drug Synergy In Vitro Study Fri, 01 Jun 2018 00:15:22 +0000 greenmedinfo 165076 at https://greenmedinfo.com Results support a relationship between cancer and altered vitamin D metabolism in dogs, https://greenmedinfo.com/article/results-support-relationship-between-cancer-and-altered-vitamin-d-metabolism-d PMID:  J Vet Intern Med. 2017 Sep 23. Epub 2017 Sep 23. PMID: 28941306 Abstract Title:  Influence of Various Factors on Circulating 25(OH) Vitamin D Concentrations in Dogs with Cancer and Healthy Dogs. Abstract:  BACKGROUND: Low blood 25-hydroxyvitamin D (25(OH)D) concentrations have been associated with cancer in dogs. Little research has examined what other factors may affect 25(OH)D concentrations.OBJECTIVES: (1) To determine whether the presence of cancer (lymphoma, osteosarcoma, or mast cell tumor [MCT]) in dogs is associated with plasma 25(OH)D concentrations and (2) identify other factors related to plasma 25(OH)D concentrations in dogs.ANIMALS: Dogs newly diagnosed with osteosarcoma (n = 21), lymphoma (n = 27), and MCT (n = 21) presented to a tertiary referral oncology center, and healthy, client-owned dogs (n = 23).METHODS: An observational study design was used. Dietary vitamin D intake, sex, age, body condition score (BCS), muscle condition score (MCS), and plasma concentrations of 25(OH)D, 24,25-dihydroxyvitamin D (24,25(OH)2 D) (a marker of CYP24A1 activity), as well as ionized calcium (ICa), parathyroid hormone, and parathyroid hormone-related protein concentrations were measured. An analysis of covariance was used to model plasma 25(OH)D concentrations.RESULTS: Cancer type (P = 0.004), plasma 24,25(OH)2 D concentrations (P<p><a href="https://greenmedinfo.com/article/results-support-relationship-between-cancer-and-altered-vitamin-d-metabolism-d" target="_blank">read more</a></p> https://greenmedinfo.com/article/results-support-relationship-between-cancer-and-altered-vitamin-d-metabolism-d#comments Animal Cancers Cancers: Canine Vitamin D Vitamin D Deficiency Risk Factors Animal Study Mon, 25 Sep 2017 23:14:19 +0000 greenmedinfo 153347 at https://greenmedinfo.com The use of oligo fucoidan in cancer bearing dogs undergoing chemotherapy. https://greenmedinfo.com/article/use-oligo-fucoidan-cancer-bearing-dogs-undergoing-chemotherapy PMID:  Top Companion Anim Med. 2021 Dec 2:100616. Epub 2021 Dec 2. PMID: 34864255 Abstract Title:  The use of Oligo Fucoidan in Cancer Bearing Dogs Undergoing Chemotherapy: a double-blinded study. Abstract:  We evaluated the effect of oligo fucoidan (Laminina Japonica) derived from oceanic brown seaweed on the quality of life in dogs with cancer undergoing chemotherapy in a double-blinded case control study. Included in this prospective study were 100 dogs with a confirmed diagnosis of cancer that were being treated with chemotherapy. Dogs were randomly assigned to be treated with oligo fucoidan (treated group; n=68) or placebo (placebo group; n=32). Dogs were evaluated every 2-3 weeks for 3 months with a complete blood count (CBC) and serum biochemistry profile, and a complete history and physical examination by blinded clinicians at The Veterinary Cancer Center. The owners of the dogs enrolled in the study were required at each visit to complete a Quality-of-Life Questionnaire specifically designed for cancer-bearing veterinary patients. The owners were also blinded as to whether their dog was receiving oligo fucoidan or placebo. There were no significant differences between the CBC parameters or the serum biochemical parameters of the dogs in the treated and placebo-controlled groups. There was no significant difference in the median quality of life scores between the two cohorts, however, when evaluating the individual quality of life metrics, five out of the 23 metrics showed statistically significant improvement and none of the quality of life metrics declined in the oligo fucoidan group as compared to the placebo group. All of the dogs that had a positive change in overall quality of life scores were dogs that received oligo fucoidan. There were minimal adverse side effects of giving the oligo fucoidan to dogs. Treatment with oligo fucoidan was safe and improved some of the quality-of-life metrics in dogs who were being treated with chemotherapy for cancer. <p><a href="https://greenmedinfo.com/article/use-oligo-fucoidan-cancer-bearing-dogs-undergoing-chemotherapy" target="_blank">read more</a></p> https://greenmedinfo.com/article/use-oligo-fucoidan-cancer-bearing-dogs-undergoing-chemotherapy#comments Cancers: Canine Fucoidan Chemotherapeutic Significant Treatment Outcome Animal Study Sun, 12 Dec 2021 01:40:55 +0000 greenmedinfo 250062 at https://greenmedinfo.com