Simian virus 40 (SV40) https://greenmedinfo.com/taxonomy/term/14510/all en "Identification of the oncogenic substance in rhesus monkey kidney cell culture as simian virus 40." https://greenmedinfo.com/article/identification-oncogenic-substance-rhesus-monkey-kidney-cell-culture-simian-vi PMID:  Virology. 1962 May ;17:65-75. PMID: 13889129 Abstract Title:  Identification of the oncogenic substance in rhesus monkey kidney cell culture as simian virus 40. Abstract:  no abstract available. https://greenmedinfo.com/article/identification-oncogenic-substance-rhesus-monkey-kidney-cell-culture-simian-vi#comments Simian virus 40 (SV40) Simian virus 40 (SV40) Viral Sat, 22 Dec 2012 18:36:43 +0000 greenmedinfo 87428 at https://greenmedinfo.com A reassessment of SV40 as a contaminant based upon legal documents. https://greenmedinfo.com/article/reassessment-sv40-contaminant-based-upon-legal-documents PMID:  Anticancer Res. 2000 Nov-Dec;20(6C):4745-9. PMID: 11205211 Abstract Title:  Oral polio vaccine and human cancer: a reassessment of SV40 as a contaminant based upon legal documents. Abstract:  To date, the scientific literature and research examining SV40 and cancer-related diseases has been based upon an assumption that SV40 was not present in any poliovirus vaccine administered in the United States and was removed from the killed polio vaccine by 1963. The basis for this presumption has been that the regulations for live oral polio vaccine required that SV40 be removed from the seeds and monovalent pools ultimately produced in the manufacturing process. The Division of Biologic Standards permitted an additional two tissue culture passages--from three to five--in order to allow manufacturers the ability to remove this contaminant from the oral poliovirus vaccines then awaiting licensure. The confirmation of the removal by one drug manufacturer, Lederle, has been made public at an international symposium in January 1997, where its representatives stated that all of Lederle&#039;s seeds had been tested and screened to assure that it was free from SV40 virus. However, in litigation involving the Lederle oral polio vaccine, the manufacturer&#039;s internal documents failed to reveal such removal in all of the seeds. The absence of confirmatory testing of the seeds, as well as testimony of a Lederle manager, indicate that this claim of removal of SV40 and the testing for SV40 in all the seeds cannot be fully substantiated. These legal documents and testimony indicate that the scientific community should not be content with prior assumptions that SV40 could not have been in the oral polio vaccine. Only further investigation by outside scientific and independent researchers who can review the test results claimed in the January 1997 meeting and who can conduct their own independent evaluations by testing all the seeds and individual mono-valent pools will assure that SV40 has not been present in commercially sold oral poliovirus vaccine manufactured by Lederle. <p><a href="https://greenmedinfo.com/article/reassessment-sv40-contaminant-based-upon-legal-documents" target="_blank">read more</a></p> https://greenmedinfo.com/article/reassessment-sv40-contaminant-based-upon-legal-documents#comments Simian virus 40 (SV40) Simian virus 40 (SV40) Review Tue, 31 Jul 2018 00:54:28 +0000 greenmedinfo 168194 at https://greenmedinfo.com CDC 'Disappears’ Page Linking Polio Vaccines To Cancer-Causing Viruses https://greenmedinfo.com/blog/cdc-disappears%E2%80%99-page-linking-polio-vaccines-cancer-causing-viruses1 <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2013<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt=" CDC 'Disappears' Page Linking Polio Vaccines To Cancer-Causing Viruses" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/Sayer Ji/images/image(4).jpg" style="width: 500px; height: 350px;" /></p> <p><span style="font-size: 0.9em;">The alternative media is justifiably abuzz with a story about the CDC deleting a page on their website admitting that as many as 98 million Americans received polio vaccine contaminated with the cancer-linked monkey virus </span>SV40<span style="font-size: 0.9em;">, with the added twist that although they removed the page sometime after July </span>11th<span style="font-size: 0.9em;">, a cached version is still available to </span><strong><a href="http://webcache.googleusercontent.com/search?q=cache:8pUR6KX_Vq8J:www.cdc.gov/vaccinesafety/updates/archive/polio_and_cancer_factsheet.htm+cdc+polio+cancer+fact+sheet&amp;cd=2&amp;hl=en&amp;ct=clnk&amp;gl=ca">view online</a></strong><span style="font-size: 0.9em;">.</span></p><p><a href="https://greenmedinfo.com/blog/cdc-disappears%E2%80%99-page-linking-polio-vaccines-cancer-causing-viruses1" target="_blank">read more</a></p> https://greenmedinfo.com/blog/cdc-disappears%E2%80%99-page-linking-polio-vaccines-cancer-causing-viruses1#comments Simian virus 40 (SV40) Vaccine-induced Toxicity Simian virus 40 (SV40) Vaccination: Oral Polio Vaccine Vaccination: Oral Polio Vaccine, Bivalent Vaccination: Polio Vaccine Rights Endogenous Retroviruses Vaccine Contamination Fri, 19 Jul 2013 00:48:24 +0000 Sayer Ji 104986 at https://greenmedinfo.com Curcumin suppresses SV40-immortalized bladder cancer cells. https://greenmedinfo.com/article/curcumin-suppresses-sv40-immortalized-bladder-cancer-cells PMID:  Anticancer Res. 2007 Mar-Apr;27(2):737-40. PMID: 17465196 Abstract Title:  Sensitivity of bladder cancer cells to curcumin and its derivatives depends on the extracellular matrix. Abstract:  Because the response of cancer cells to chemotherapeutic agents depends upon the supporting extracellular matrix (ECM), the response in vivo may not be reproduced in 2-dimensional cell culture. The dose-response to curcumin and two derivatives by bladder cancer cells grown on both normal (SISgel) and cancer-derived ECM (Matrigel) and on plastic were contrasted. Cells grown on Matrigel were resistant to curcumins, but cells growing on SISgel, which mimic cancer cells suppressed by normal ECM, were nearly as sensitive as cells grown on plastic. SV40-immortalized urothelial cells, which are models for premalignant cells, were the most sensitive, but even aggressive cell lines were nearly as sensitive when grown on SISgel as on plastic. Curcumin response depends highly on the supporting ECM, and cells grown on plastic poorly models cells growing on natural ECM. Curcumin could prove an effective chemopreventive for bladder cancer recurrence when administered intravesically post-therapy. https://greenmedinfo.com/article/curcumin-suppresses-sv40-immortalized-bladder-cancer-cells#comments Bladder Cancer Curcumin Simian virus 40 (SV40) Antineoplastic Agents Apoptotic In Vitro Study Sat, 17 Apr 2010 14:34:48 +0000 greenmedinfo 54946 at https://greenmedinfo.com Flavonoids purified from Rhus verniciflua Stokes (Sumac) exhibit selective antiproliferative and apoptotic effects on SV40-transformed liver tumor cells. https://greenmedinfo.com/article/flavonoids-purified-rhus-verniciflua-stokes-sumac-exhibit-selective-antiprolif PMID:  Toxicol Lett. 2005 Jan 15;155(1):115-25. PMID: 15585366 Abstract Title:  Selective antiproliferative and apoptotic effects of flavonoids purified from Rhus verniciflua Stokes on normal versus transformed hepatic cell lines. Abstract:  Considerable attention is being concentrated on dietary flavonoids in developing novel cancer-preventive approaches due to their potential ability to induce selective apoptosis of cancer cells. In this study, we prepared a flavonoid-containing fraction from a crude acetone extract of Rhus verniciflua Stokes (RVS), traditionally used as a food additive and as an herbal medicine, and named RVS chloroform-methanol fraction (RCMF). We evaluated the effects of RCMF on proliferation and apoptosis using mouse embryonic primary hepatic cells (MPHC), embryonic normal hepatic cell line (BNL CL.2), and its SV40-mediated transformed cell line (BNL SV A.8). We also investigated the effects of RCMF on the antioxidant defense system in those cells. This study demonstrated that RCMF exhibited a selective growth inhibition and apoptosis induction on transformed cells. BNL SV A.8 cells were more sensitive to RCMF-mediated cytotoxicity than were MPHC or BNL CL.2. RCMF-mediated reduction of MnSOD activity and glutathione (GSH) content in BNL SV A.8 cells is thought to be associated with RCMF-induced apoptosis. Our findings suggest that RCMF is an agent which may be capable of inducing growth inhibition and apoptosis of hepatic tumor cells. https://greenmedinfo.com/article/flavonoids-purified-rhus-verniciflua-stokes-sumac-exhibit-selective-antiprolif#comments Flavonoids Liver Cancer Simian virus 40 (SV40) Sumac Antioxidants Selective Cytotoxicity In Vitro Study Mon, 18 Jan 2010 02:11:40 +0000 greenmedinfo 49600 at https://greenmedinfo.com Green tea catechins and black tea theaflavins inhibit SV40-associated mammary cancer in a rodent experimental model. https://greenmedinfo.com/article/green-tea-catechins-and-black-tea-theaflavins-inhibit-sv40-associated-mammary- PMID:  J Agric Food Chem. 2007 May 2;55(9):3378-85. Epub 2007 Apr 4. PMID: 17407311 Abstract Title:  Breast cancer prevention by green tea catechins and black tea theaflavins in the C3(1) SV40 T,t antigen transgenic mouse model is accompanied by increased apoptosis and a decrease in oxidative DNA adducts. Abstract:  Tea consumption is associated with a reduced risk of mammary cancer as reflected by epidemiological studies and experiments in carcinogen-induced rodent models of mammary carcinogenesis. We tested the hypothesis that green tea catechins (GTC) or theaflavins from black tea (BTT) interfere with mammary carcinogenesis in C3(1) SV40 T,t antigen transgenic multiple mammary adenocarcinoma (TAg) mice and that GTC/BTT affect tumor survival or oxidation status. TAg mice received GTC/BTT (0.05%) in drinking water for their lifetime. As compared to control mice, they survived longer and had smaller tumors. On microscopic inspection, the size of the largest tumor per mouse was decreased by 40-42% (p https://greenmedinfo.com/article/green-tea-catechins-and-black-tea-theaflavins-inhibit-sv40-associated-mammary-#comments Black Tea Breast Cancer Catechin Green Tea Simian virus 40 (SV40) Apoptotic Chemopreventive Animal Study Sat, 17 Apr 2010 14:36:39 +0000 greenmedinfo 54947 at https://greenmedinfo.com Green tea inhibits mammary tumorigenesis in the SV40-induced mouse model. https://greenmedinfo.com/article/green-tea-inhibits-mammary-tumorigenesis-sv40-induced-mouse-model PMID:  Breast Cancer Res Treat. 2008 Feb;107(3):359-69. Epub 2007 May 5. PMID: 17484049 Abstract Title:  Inhibition of mammary tumorigenesis in the C3(1)/SV40 mouse model by green tea. Abstract:  Previous studies show inhibitory effects of green tea in chemically induced mammary tumors or human tumor explants, but not in spontaneous tumor models that are more representative of human breast cancer. The C3(1)/SV40 mouse model is particularly suited for breast cancer prevention studies because it produces spontaneous ductal adenocarcinomas and a predictable time course for mammary tumorigenesis through a multistage progression similar to that occurring in humans. We therefore used this model to test the chemoprotective effects of green tea. Administration of 0.5% Polyphenon E (Poly E) (a standardized preparation of green tea extract) in drinking water delayed tumor onset and suppressed tumor growth by 40%, compared to tap water-fed animals, with no adverse side effects. Histological analysis of mammary glands showed that green tea slowed the progression of ductal lesions to advanced mammary intraepithelial neoplasias and suppressed tumor invasiveness. Green tea inhibited the proliferation of ductal epithelial cells and tumors and, overall, disrupted post-pubertal ductal growth. Immunohistochemical analyses also demonstrated that green tea inhibited angiogenesis through a decrease in both ductal epithelial and stromal VEGF expression and a decrease in intratumoral microvascular density. Our data strongly support the potential use of green tea as a breast cancer chemopreventive agent. https://greenmedinfo.com/article/green-tea-inhibits-mammary-tumorigenesis-sv40-induced-mouse-model#comments Breast Cancer Green Tea Simian virus 40 (SV40) Anti-Angiogenic Vascular Endothelial Growth Factor Regulator Diseases that are Linked Animal Study Mon, 18 Jan 2010 03:38:17 +0000 greenmedinfo 49628 at https://greenmedinfo.com Immunological data from this study suggests that SV40 could be causing human infections. https://greenmedinfo.com/article/immunological-data-study-suggests-sv40-could-be-causing-human-infections PMID:  PLoS One. 2016 ;11(1):e0145720. Epub 2016 Jan 5. PMID: 26731525 Abstract Title:  Specific Antibodies Reacting with SV40 Large T Antigen Mimotopes in Serum Samples of Healthy Subjects. Abstract:  Simian Virus 40, experimentally assayed in vitro in different animal and human cells and in vivo in rodents, was classified as a small DNA tumor virus. In previous studies, many groups identified Simian Virus 40 sequences in healthy individuals and cancer patients using PCR techniques, whereas others failed to detect the viral sequences in human specimens. These conflicting results prompted us to develop a novel indirect ELISA with synthetic peptides, mimicking Simian Virus 40 capsid viral protein antigens, named mimotopes. This immunologic assay allowed us to investigate the presence of serum antibodies against Simian Virus 40 and to verify whether Simian Virus 40 is circulating in humans. In this investigation two mimotopes from Simian Virus 40 large T antigen, the viral replication protein and oncoprotein, were employed to analyze for specific reactions to human sera antibodies. This indirect ELISA with synthetic peptides from Simian Virus 40 large T antigen was used to assay a new collection of serum samples from healthy subjects. This novel assay revealed that serum antibodies against Simian Virus 40 large T antigen mimotopes are detectable, at low titer, in healthy subjects aged from 18-65 years old. The overall prevalence of reactivity with the two Simian Virus 40 large T antigen peptides was 20%. This new ELISA with two mimotopes of the early viral regions is able to detect in a specific manner Simian Virus 40 large T antigen-antibody responses. https://greenmedinfo.com/article/immunological-data-study-suggests-sv40-could-be-causing-human-infections#comments Simian virus 40 (SV40) Human Study Fri, 29 Jan 2016 00:46:14 +0000 greenmedinfo 123519 at https://greenmedinfo.com India's Polio-Free Status a Cruel Joke https://greenmedinfo.com/blog/indias-polio-free-status-cruel-joke <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2015<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><br /> <img alt="India's Polio-Free Status a Cruel Joke" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/Sayer Ji/images/Screen%20Shot%202015-06-05%20at%208_49_26%20AM.png" style="width: 400px; height: 300px;" /></p> <p class="rtecenter"><strong>Photo:&nbsp;<a href="/blog/questioning-dalai-lama-who-would-buddha-vaccinate1" target="_blank">The Dalai Lama launching the bivalent oral polio vaccine in India, Jan 2010. Learn More</a></strong>.</p> <p><span style="font-size:18px;"><strong><em>The definition of polio has been changed repeatedly since the programme was launched, thus automatically leading to a drastic fall in the number of cases</em></strong></span></p><p><a href="https://greenmedinfo.com/blog/indias-polio-free-status-cruel-joke" target="_blank">read more</a></p> https://greenmedinfo.com/blog/indias-polio-free-status-cruel-joke#comments Polio Polio: Vaccine-Related Poliomyelitis Simian virus 40 (SV40) Vaccine-induced Toxicity Vaccination: Oral Polio Vaccine Vaccination: Oral Polio Vaccine, Bivalent Vaccination: Polio Vaccine Effects Vaccine Information Center Vaccine Rights Fri, 05 Jun 2015 13:03:02 +0000 jag.Chatterjee 118054 at https://greenmedinfo.com Polio, hepatitis B and AIDS: an integrative theory on a possible vaccine induced pandemic. https://greenmedinfo.com/article/polio-hepatitis-b-and-aids-integrative-theory-possible-vaccine-induced-pandemi PMID:  Med Hypotheses. 2001 May ;56(5):677-86. PMID: 11388787 Abstract Title:  Polio, hepatitis B and AIDS: an integrative theory on a possible vaccine induced pandemic. Abstract:  The hypothesis that simian virus 40 (SV40) infected polio vaccines may be linked to the evolution of acquired immunodeficiency disorder (AIDS), and certain cancers, has been advanced. Most recently, investigators discussed the likelihood of gene-reshuffling following SV40 infection as a precursor to acquired immune dysfunction. Findings of recent SV40 infections in four children born after 1982 suggest infections were transmitted vertically along gene lines. Earlier observations proved activation of a retrovirus gene by a hepatitis B virus (HBV) protein. This paper proposes a new integrative theory on the origin of AIDS. It advances the possibility of genetic recombinations with oncogene activation by HBV involving simian viruses that likely infected polio vaccinated blood donors to the initial hepatitis B (HB) vaccine trials conducted on gay men in New York City and Ugandan Blacks in the early to mid-1970s. The socio-economic and even military ramifications associated with this politically challenging thesis are discussed. <p><a href="https://greenmedinfo.com/article/polio-hepatitis-b-and-aids-integrative-theory-possible-vaccine-induced-pandemi" target="_blank">read more</a></p> https://greenmedinfo.com/article/polio-hepatitis-b-and-aids-integrative-theory-possible-vaccine-induced-pandemi#comments Simian virus 40 (SV40) Vaccine-induced Toxicity Vaccination: Hepatitis B Vaccination: Polio Commentary Sat, 20 Oct 2018 21:51:20 +0000 greenmedinfo 172765 at https://greenmedinfo.com Recent data indicate that the Simian virus 40 infection appears to be transmitted in humans independently from early SV40-contaminated antipolio vaccines. https://greenmedinfo.com/article/recent-data-indicate-simian-virus-40-infection-appears-be-transmitted-humans-i PMID:  J Cell Physiol. 2019 Apr ;234(4):3170-3179. Epub 2018 Oct 26. PMID: 30362540 Abstract Title:  Antibodies reacting to mimotopes of Simian virus 40 large T antigen, the viral oncoprotein, in sera from children. Abstract:  Recent data indicate that the Simian virus 40 (SV40) infection appears to be transmitted in humans independently from early SV40-contaminated antipolio vaccines. Serum antibodies against SV40 large T antigen (Tag) were analyzed in children/adolescents and young adults. To investigate antibodies reacting to SV40 Tag antigens, serum samples ( n = 812) from children and young adults were analyzed by indirect ELISAs using specific SV40 Tag mimotopes. Mimotopes were synthetic peptides corresponding to SV40 Tag epitopes. In sera ( n = 412) from healthy children up to 17 years old, IgG antibodies against SV40 Tag mimotopes reached an overall prevalence of 15%. IgM antibodies against SV40 Tag were detected in sera of children 6-8 months old confirming and extending the knowledge that SV40 seroconversion occurs early in life. In children/adolescents affected by different diseases ( n = 180) SV40 Tag had a prevalence of 18%, being the difference no significant compared to healthy subjects ( n = 220; 16%) of the same age. Our immunological data indicate that SV40 circulates in children and young adults, both in healthy conditions and affected by distinct diseases. The IgM detection in sera from healthy children suggeststhat the SV40 infection/seroconversion occurs early in life (&gt;6 months). Our immunological data support the hypothesis that SV40, or a closely related still unknown polyomavirus, infects humans. The SV40 seroprevalence is lower than common polyomaviruses, such as BKPyV and JCPyV, and other new human polyomaviruses. In addition, our immunological surveillance indicates a lack of association between different diseases, considered herein, and SV40. <p><a href="https://greenmedinfo.com/article/recent-data-indicate-simian-virus-40-infection-appears-be-transmitted-humans-i" target="_blank">read more</a></p> https://greenmedinfo.com/article/recent-data-indicate-simian-virus-40-infection-appears-be-transmitted-humans-i#comments Simian virus 40 (SV40) Human Study Wed, 13 Mar 2019 00:10:41 +0000 greenmedinfo 181271 at https://greenmedinfo.com Simian virus 40 and human cancer. https://greenmedinfo.com/article/simian-virus-40-and-human-cancer PMID:  Monaldi Arch Chest Dis. 1998 Apr ;53(2):198-201. PMID: 9689809 Abstract Title:  Simian virus 40 and human cancer. Abstract:  Deoxyribonucleic acid (DNA) oncoviruses can induce neoplastic transformation by interfering with proliferative proteins. Simian virus 40 (SV40) has been shown to induce brain tumors, osteosarcoma, lymphoid tumors and malignant mesothelioma in hamsters and SV40-like DNA sequences corresponding to the Rb-pocket binding domain of SV40 T-antigen (Tag) have been detected in the same human tumors. Since only a small percentage of people exposed to asbestos fibers develop a malignant mesothelioma, SV40 has been suspected to co-operate with the fibers in the neoplastic transformation or even to itself induce the onset of malignant mesothelioma in patients without expositive history. The mechanism that seems to be involved in the SV40-induced carcinogenesis process is mediated by interaction of Tag, both with p53 and Rb proteins, leading to their functional inactivation that is responsible for the removal of their inhibitory cell cycle effect which determines the increase of the number of cells entering the G1-S phase. Up to now the source of SV40 human infections has not yet been completely identified even though administration from 1957-1965 of SV40 contaminated polio vaccines is highly suspected. Horizontal infection by sexual transmission has been also hypothesized. Due to the important public health implications further investigations are required in order to establish both the source and the carcinogenetic role of simian virus 40 in humans. <p><a href="https://greenmedinfo.com/article/simian-virus-40-and-human-cancer" target="_blank">read more</a></p> https://greenmedinfo.com/article/simian-virus-40-and-human-cancer#comments Cancers: All Simian virus 40 (SV40) Cell cycle arrest Simian virus 40 (SV40) Vaccination: Polio Review Tue, 31 Jul 2018 00:03:42 +0000 greenmedinfo 168187 at https://greenmedinfo.com Simian virus 40 can induce cancers in hamsters https://greenmedinfo.com/article/simian-virus-40-can-induce-cancers-hamsters PMID:  Dev Biol Stand. 1998 ;94:273-9. PMID: 9776247 Abstract Title:  Simian virus 40 oncogenesis in hamsters. Abstract:  Simian virus 40 (SV40) is a DNA tumour virus which is highly oncogenic in hamsters. Only specific histologic types of tumours develop in hamsters injected with SV40, and these are influenced by the route of virus inoculation. When SV40 is injected systemically to expose most different cell types to the virus, the animals develop mesotheliomas, osteosarcomas, sarcomas, and lymphomas within six months. When the virus is injected subcutaneously, sarcomas at the site of injection develop. If hamsters are injected intracranially with SV40, they develop ependymomas. These same tumour types have been found to contain SV40. https://greenmedinfo.com/article/simian-virus-40-can-induce-cancers-hamsters#comments Lymphoma Mesothelioma Osteosarcoma Simian virus 40 (SV40) Carcinogenic Simian virus 40 (SV40) Animal Study Mon, 02 Apr 2012 20:40:37 +0000 greenmedinfo 73857 at https://greenmedinfo.com Simian virus 40 efficiently infects human T lymphocytes and both extends their lifespan and alters their morphology. https://greenmedinfo.com/article/simian-virus-40-efficiently-infects-human-t-lymphocytes-and-both-extends-their PMID:  Exp Hematol. 2012 Mar 12. Epub 2012 Mar 12. PMID: 22421183 Abstract Title:  Simian virus 40 efficiently infects human T lymphocytes and extends their lifespan. Abstract:  The relevance of viral infections to the onset/progression of human hematologic malignancies and other blood diseases is still a matter of active investigation. Purified human T lymphocytes isolated from the peripheral blood mononuclear cells of healthy blood donors were experimentally infected with simian virus 40 (SV40), a small DNA tumor virus. SV40-positive T lymphocytes extended their lifespan up to day 80 post-infection (p.i.). Expression of viral antigens, such as the large T antigen and the viral capsid protein VP1 from the early and late regions, respectively, was detected up to day 40 p.i. SV40 viral progeny were continuously produced from day 10 to 40 p.i. SV40 DNA sequences were detected in infected T cells for up to 80 days. Our data indicate that human T lymphocytes can be efficiently infected with SV40. While T cells infected by SV40 were not immortalized, 30% of these lymphocytes appeared to be morphologically transformed with an enlarged T cell shape. Our investigation provides a simple model for studying the interactions of human T lymphocytes with this small DNA tumor virus and it may represent an experimental tool for investigating new biomarkers and targets for innovative therapeutic approaches. https://greenmedinfo.com/article/simian-virus-40-efficiently-infects-human-t-lymphocytes-and-both-extends-their#comments Simian virus 40 (SV40) T lymphocytes: Morphological Abnormalities Simian virus 40 (SV40) Human In Vitro Mon, 02 Apr 2012 19:07:57 +0000 greenmedinfo 73851 at https://greenmedinfo.com Some oral poliovirus vaccines were contaminated with infectious SV40 after 1961. https://greenmedinfo.com/article/some-oral-poliovirus-vaccines-were-contaminated-infectious-sv40-after-1961 PMID:  Cancer Res. 2005 Nov 15 ;65(22):10273-9. PMID: 16288015 Abstract Title:  Some oral poliovirus vaccines were contaminated with infectious SV40 after 1961. Abstract:  Some polio vaccines prepared from 1954 to 1961 were contaminated with infectious SV40. It has been assumed that all polio vaccines were SV40 free in the United States after 1961 and in other countries after 1962. Following a WHO requirement that was prompted by the detection of SV40 in some human tumors, we conducted a multilaboratory study to test for SV40 polio vaccines prepared after 1961. Vaccine samples from 13 countries and the WHO seed were initially tested by PCR. The possible presence of intact and/or infectious SV40 DNA in PCR-positive samples was tested by transfection and infection of permissive CV-1 cells. All results were verified by immunohistochemistry, cloning, and sequencing. All the vaccines were SV40 free, except for vaccines from a major eastern European manufacturer that contained infectious SV40. We determined that the procedure used by this manufacturer to inactivate SV40 in oral poliovirus vaccine seed stocks based on heat inactivation in the presence of MgCl2 did not completely inactivate SV40. These SV40-contaminated vaccines were produced from early 1960s to about 1978 and were used throughout the world. Our findings underscore the potential risks of using primary monkey cells for preparing poliovirus vaccines, because of the possible contamination with SV40 or other monkey viruses, and emphasize the importance of using well-characterized cell substrates that are free from adventitious agents. Moreover, our results indicate possible geographic differences in SV40 exposure and offer a possible explanation for the different percentage of SV40-positive tumors detected in some laboratories. https://greenmedinfo.com/article/some-oral-poliovirus-vaccines-were-contaminated-infectious-sv40-after-1961#comments Simian virus 40 (SV40) Simian virus 40 (SV40) Vaccination: Polio Vaccine Contamination Human In Vitro Mon, 02 Apr 2012 21:07:11 +0000 greenmedinfo 73860 at https://greenmedinfo.com