Diabetic Nephropathy https://greenmedinfo.com/taxonomy/term/1671/all en Crocin ameliorates oxidative stress and suppresses renal damage in streptozotocin induced diabetic male rats. https://greenmedinfo.com/article/crocin-ameliorates-oxidative-stress-and-suppresses-renal-damage-streptozotocin PMID:  Biotech Histochem. 2020 Aug 24:1-8. Epub 2020 Aug 24. PMID: 32835521 Abstract Title:  Crocin ameliorates oxidative stress and suppresses renal damage in streptozotocin induced diabetic male rats. Abstract:  Diabetes mellitus (DM) is a prevalent metabolic disorder with complications including nephropathy, neuropathy, retinopathy and peripheral vascular disease. Crocin is a water soluble carotenoid that exhibits strong antioxidant activity. I investigated the potential hypoglycemic and reno-protective effects of crocin for type 1 diabetic male rats using periodic acid-Schiff and hematoxylin staining; metalloproteinase-7 (MMP-7), phosphatase and tensin homolog (PTEN) and phospho-Akt (p-Akt) immunohistochemistry; measurement of blood glucose, malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant and total oxidant status; and oxidative stress index. I used four groups of rats: control (saline treated), diabetic (single dose of 65 mg/kg streptozotocin), crocin (treated with 50 mg/kg) and diabetic + crocin. Crocin decreased levels of MDA, SOD, oxidative stress index and glucose significantly in diabetic animals. Renal damage from DM also was decreased by crocin treatment. MMP-7 and p-Akt immunoreactivity were stronger in the diabetic group compared to other groups, but PTEN immunoreactivity was weaker. Crocin treatment returned MMP-7 and PTEN expression to near normal. The ameliorative effect of crocin is attributed to its stimulation of the antioxidant defense system and its ability to regulate the MMP-7/PTEN/Akt signaling cascade. <p><a href="https://greenmedinfo.com/article/crocin-ameliorates-oxidative-stress-and-suppresses-renal-damage-streptozotocin" target="_blank">read more</a></p> https://greenmedinfo.com/article/crocin-ameliorates-oxidative-stress-and-suppresses-renal-damage-streptozotocin#comments Crocin Diabetic Nephropathy Matrix Metalloproteinase-7 (MMP-7) Inhibitor Renoprotective Animal Study Thu, 27 Aug 2020 20:23:49 +0000 greenmedinfo 225837 at https://greenmedinfo.com Therapeutic role of Azadirachta indica leaves ethanolic extract against diabetic nephropathy in rats neonatally induced by streptozotocin. https://greenmedinfo.com/article/therapeutic-role-azadirachta-indica-leaves-ethanolic-extract-against-diabetic- PMID:  Ultrastruct Pathol. 2021 Nov 2 ;45(6):391-406. Epub 2021 Oct 31. PMID: 34720017 Abstract Title:  Therapeutic role ofleaves ethanolic extract against diabetic nephropathy in rats neonatally induced by streptozotocin. Abstract:  Diabetic nephropathy (DN) is manifested by chronic loss of renal function due to damage of glomeruli and renal tubules. Therefore, this study is mainly designed to evaluate the therapeutic role of(neem) leaves extract as a novel approach for treatment of DN in rats neonatally induced by streptozotocin (STZ). For this study, 40 offspring were selected after parturition and categorized into four groups (n = 10). Group1: control group, group 2: neem leaves extract supplemented group, group 3: diabetic group that injected with a single dose of STZ and group 4: diabetic group treated with neem extract. The results revealed deleterious histological and ultrstructural changes in the renal tissues of diabetic rats. Such changes included atrophied glomeruli, dilated renal cortical tubules and scattered hemorrhage spots, thickening of glomerular basement membrane, expansion of mesangial matrix and pyknotic podocyte. Additionally, the proximal convoluted tubule and distal tubule showed cytoplasmic vacuolation, vacuolated mitochondria, scattered lipid droplets, lost microvilli and disrupted basal lamina and basal infoldings. Moreover, significant decreased levels of serum antioxidants (SOD&amp;CAT) and significant increased levels of serum MDA, urea and creatinine were noticed in diabetic rats. Neem leaves extract successfully alleviated the histological and ultrastructural as well as biochemical changes induced by diabetes. <p><a href="https://greenmedinfo.com/article/therapeutic-role-azadirachta-indica-leaves-ethanolic-extract-against-diabetic-" target="_blank">read more</a></p> https://greenmedinfo.com/article/therapeutic-role-azadirachta-indica-leaves-ethanolic-extract-against-diabetic-#comments Diabetic Nephropathy Neem Antioxidants Catalase Up-Regulation Renoprotective Superoxide Dismutase Up-regulation Plant Extracts Animal Study Wed, 15 Jun 2022 16:42:28 +0000 greenmedinfo 259096 at https://greenmedinfo.com "Prevention of diabetic nephropathy by sulforaphane: possible role of Nrf2 upregulation and activation." https://greenmedinfo.com/article/prevention-diabetic-nephropathy-sulforaphane-possible-role-nrf2-upregulation-a PMID:  Oxid Med Cell Longev. 2012 ;2012:821936. Epub 2012 Sep 23. PMID: 23050040 Abstract Title:  Prevention of diabetic nephropathy by sulforaphane: possible role of Nrf2 upregulation and activation. Abstract:  The present study was to investigate whether sulforaphane (SFN) can prevent diabetic nephropathy in type 1 diabetic mouse model induced by multiple low-dose streptozotocin. Diabetic and age-matched control mice were given SFN at 0.5 mg/kg body weight daily for 3 months. At the end of 3-month SFN treatment, the diabetic nephropathy, shown by renal inflammation, oxidative damage, fibrosis, and dysfunction, was significantly prevented along with an elevation of renal Nrf2 expression and transcription in diabetes/SFN group compared with diabetic group. However, this renal prevention by SFN was not seen when the 3-month SFN-treated diabetic mice were aged for additional 3 months without further SFN treatment. Nrf2-mediated renal protective effects in diabetes were evaluated in human renal tubular HK11 cells transfected with control and Nrf2 siRNA and treated with 27.5 mM mannitol or high glucose plus palmitate (300μM). Blockade of Nrf2 expression completely abolished SFN prevention of the profibrotic effect induced by high glucose plus palmitate. These results support that renal Nrf2 expression and its transcription play important roles in SFN prevention of diabetes-induced renal damage. However, the SFN preventive effect on diabetes-induced renal pathogeneses is not sustained, suggesting the requirement of continual use of SFN for its sustained effect. https://greenmedinfo.com/article/prevention-diabetic-nephropathy-sulforaphane-possible-role-nrf2-upregulation-a#comments Diabetic Nephropathy Sulforaphane Animal Study Wed, 19 Dec 2012 17:42:57 +0000 greenmedinfo 87175 at https://greenmedinfo.com 2,3,5,4'-Tetrahydroxystilbene-2-O-β-d-glucoside exerted protective effects on diabetic nephropathy. https://greenmedinfo.com/article/2354-tetrahydroxystilbene-2-o-d-glucoside-exerted-protective-effects-diabetic- PMID:  Food Funct. 2016 Nov 9 ;7(11):4628-4636. PMID: 27747335 Abstract Title:  2,3,5,4&#039;-Tetrahydroxystilbene-2-O-β-d-glucoside exerted protective effects on diabetic nephropathy in mice with hyperglycemia induced by streptozotocin. Abstract:  2,3,5,4&#039;-Tetrahydroxystilbene-2-O-β-d-glucoside (TSG), an active component from the functional and medicinal herb Polygonum multiflorum Thunb, has the capacity of blocking angiotensin II (ANG II) signaling, a pathway within the renin-angiotensin system (RAS) which plays a critical role in the development of diabetic nephropathy (DN), and blockade of the RAS is currently used for the treatment of DN. Here we investigated the beneficial effect of TSG therapy on renal damage in the streptozotocin (STZ)-induced diabetes model. The STZ-treated C57BL/6J diabetic mice developed progressive albuminuria and renal tubular interstitialfibrosis within 10 weeks, accompanied by increased production of ANG II, fibronection, TGF-β, CTGF, TNF-α, RANTES and MCP-1 and decreased expression of slit diaphragm proteins in the kidney. The treatment of the diabetic mice with a TSG ameliorated kidney mass increase prevented albuminuria, and reduced tubular interstitial fibrosis. The TSG treatment suppressed the induction of fibronection, CTGF, TGF-β, and MCP-1 and reversed the decline of slit diaphragm proteins Neph-1, ZO-1, and FAT-1. These were accompanied by blockade of renal renin and ANG II accumulation induced by hyperglycemia. These data demonstrated that the inhibition of the RAS with TSG effectively prevented renal injury in diabetic nephropathy. <p><a href="https://greenmedinfo.com/article/2354-tetrahydroxystilbene-2-o-d-glucoside-exerted-protective-effects-diabetic-" target="_blank">read more</a></p> https://greenmedinfo.com/article/2354-tetrahydroxystilbene-2-o-d-glucoside-exerted-protective-effects-diabetic-#comments Diabetic Nephropathy Renoprotective Animal Study Tue, 15 Jan 2019 02:59:08 +0000 greenmedinfo 177559 at https://greenmedinfo.com 6-Gingerol attenuates renal damage in streptozotocin-induced diabetes. https://greenmedinfo.com/article/6-gingerol-attenuates-renal-damage-streptozotocin-induced-diabetes PMID:  Pharmaceutics. 2021 Feb 28 ;13(3). Epub 2021 Feb 28. PMID: 33670981 Abstract Title:  6-Gingerol, a Bioactive Compound of Ginger Attenuates Renal Damage in Streptozotocin-Induced Diabetic Rats by Regulating the Oxidative Stress and Inflammation. Abstract:  The aim of present study is to investigate the role of 6-gingerol in ameliorating the renal injury in streptozotocin (STZ)-induced diabetic rats. The diabetes was induced by using a single dose of freshly prepared STZ (55 mg/kg body weight) intraperitoneally which causes the degeneration of pancreatic Langerhans isletβ-cells. The diabetic rats were treated with oral gavage of 6-gingerol (10 mg/kg b.w.). The treatment plan was continued for 8 weeks successively and the body weight and fasting blood glucose levels were weekly checked. The biochemical parameters like lipid profile, kidney profile, antioxidant enzyme levels, lipid peroxidation and anti-inflammatory marker levels were investigated after the treatment plant. The pathological condition of kidneys was examined by haematoxylin-eosin (H&amp;E) staining besides this analysis of NF-B protein expression by immuno-histochemistry was performed. Some of the major parameters in diabetes control vs. normal control were reported as fasting blood glucose (234± 10 vs. 102 ± 8 mg/dL), serum creatinine (109.7 ± 7.2 vs. 78.9 ± 4.5 μmol/L) and urea (39.9 ± 1.8 vs. 18.6 mg/dL), lipid profile levels were significantly enhanced in diabetic rats. Moreover, diabetic rats were marked with decreased antioxidant enzyme levels and increased inflammatory markers. Treatment with 6-gingerol significantly restored the fasting blood glucose level, hyperlipidaemia,() and inflammatory marker levels, NF-B protein expression and augmented the antioxidant enzyme levels in the kidneys of diabetic rats. The kidney damage was significantly normalized by the treatment of 6-gingerol and it provides an evidence that this novel compound plays a significant role in the protection of kidney damage. These findings demonstrate that 6-gingerol reduces lipid parameters, inflammation and oxidative stress in diabetic rats, thereby inhibiting the renal damage. Our results demonstrate that use of 6-gingerol could be a novel therapeutic approach to prevent the kidney damage associated with the diabetes mellitus. <p><a href="https://greenmedinfo.com/article/6-gingerol-attenuates-renal-damage-streptozotocin-induced-diabetes" target="_blank">read more</a></p> https://greenmedinfo.com/article/6-gingerol-attenuates-renal-damage-streptozotocin-induced-diabetes#comments Diabetic Nephropathy Gingerol Anti-Inflammatory Agents Antioxidants NF-kappaB Inhibitor Renoprotective Animal Study Sat, 08 May 2021 03:29:31 +0000 greenmedinfo 239313 at https://greenmedinfo.com 6-Shogaol ameliorates diabetic nephropathy through anti-inflammatory, hyperlipidemic, anti-oxidative activity. https://greenmedinfo.com/article/6-shogaol-ameliorates-diabetic-nephropathy-through-anti-inflammatory-hyperlipi PMID:  Biomed Pharmacother. 2018 Jan ;97:633-641. Epub 2017 Nov 6. PMID: 29101807 Abstract Title:  6-Shogaol ameliorates diabetic nephropathy through anti-inflammatory, hyperlipidemic, anti-oxidative activity in db/db mice. Abstract:  The prevalence of type 2 diabetes mellitus has been increasing worldwide and more than two thirds of the patients may develop diabetic nephropathy (DN). However, the efficiency of existing approaches on DN progression is limited. 6-Shogaol (6-SG), a major dehydrated derivative of gingerols, possesses various biological properties. The present study was designed to evaluate the possible effects of 6-SG on DN in db/db mice and to investigate the mechanisms. We revealed that 6-SG reduced the levels of fasting blood glucose, serum insulin, C-peptide, glycosylated hemoglobin A1c, and systolic blood pressure. 6-SG decreased the levels of blood urea nitrogen (BUN), serum creatinine, urinary albumin content and albumin/creatinine ratio (ACR), ameliorated the pathological injuries of kidneys, reduced the surface area of Bowman&#039;s capsule, Bowman&#039;s space, glomerular tuft, and decreased the expression of collagen IV and fibronectin in kidneys of db/db mice. The high levels of systemic and renal triglyceride and cholesterol were decreased by 6-SG. Moreover, 6-SG exhibited anti-inflammatory effects, as reflected by reduction of tumor necrosis factorɑ (TNFɑ), monocyte chemotactic protein-1 (MCP-1), and IL-6 levels in circulation and kidneys, and decrease of NF-κB expression. Furthermore, 6-SG also inhibited oxidative stress and restored the expression of NF-E2-related factor 2 (Nrf2) in kidneys of db/db mice. In conclusion, we have demonstrated that 6-SG exhibits anti-diabetic and renal protective effects against DN, in which effect the anti-inflammatory, hyperlipidemic, anti-oxidative activities may be involved. Overall, 6-SG could be a promising therapeutic treatment to ameliorate diabetes and the development of DN. <p><a href="https://greenmedinfo.com/article/6-shogaol-ameliorates-diabetic-nephropathy-through-anti-inflammatory-hyperlipi" target="_blank">read more</a></p> https://greenmedinfo.com/article/6-shogaol-ameliorates-diabetic-nephropathy-through-anti-inflammatory-hyperlipi#comments 6-Shogaol Diabetic Nephropathy Anti-Inflammatory Agents Antioxidants Hypolipidemic Interleukin-6 Downregulation NF-kappaB Inhibitor Tumor Necrosis Factor (TNF) Alpha Inhibitor Animal Study Thu, 01 Feb 2018 22:30:49 +0000 greenmedinfo 159167 at https://greenmedinfo.com 6-shogaol may be of value in protection against diabetic complications in kidney and heart. https://greenmedinfo.com/article/6-shogaol-may-be-value-protection-against-diabetic-complications-kidney-and-he PMID:  Pak J Pharm Sci. 2018 Sep ;31(5(Supplementary)):2109-2117. PMID: 30393220 Abstract Title:  6-shogaol protects against diabetic nephropathy and cardiomyopathy via modulation of oxidative stress/NF-κB pathway. Abstract:  Diabetes dramatically increases the risk of numerous heart and kidney troubles. Diabetic nephropathy (DN) and cardiomyopathy (DC) are major causes of death. The pathophysiology of DN/DC includes inflammatory and oxidative stress mechanisms. NF-κB is one of the transcription factor that mediates signal transduction processes. Nowadays, it is suggested that inhibition of NF-κB activation could delay the development of DN and DC. 6-shogaol was reported to modulate NF-κB besides its anti-oxidant and anti-inflammatory activities. Therefore,it is worth testing it against diabetic complications. Rats were divided to 4 groups: Normal control (NC), 6-shogaol (6S), diabetic control (DC), diabetic rats treated with 6-shogaol (DC+6S). BGL, BUN, serum creatinine, total urine protein, creatine kinase (CK), LDH, NO, TNF-α NF-κB were determined in serum. Heart and kidney tissues were isolated for GSH, MDA, SOD measurement and histopathology. NF-κB was estimated in kidney tissues using immunohistopathology and western blot techniques. Results showed that diabetic rats left untreated for 16 weeks showed kidney injury as evidenced from elevated BUN, serum creatinine, urine protein, TNF-α and NF-κB. Heart tissue damage was evidence from elevated CK, LDH. Diabetic rats simultaneously treated with 6-shogaol showed a protective effect on both kidney and heart as evidenced biochemically and histopathologically. Therefore, using 6-shogaol may be of value in protection against diabetic complications in kidney and heart of rats. <p><a href="https://greenmedinfo.com/article/6-shogaol-may-be-value-protection-against-diabetic-complications-kidney-and-he" target="_blank">read more</a></p> https://greenmedinfo.com/article/6-shogaol-may-be-value-protection-against-diabetic-complications-kidney-and-he#comments Cardiomyopathy Diabetic Nephropathy Anti-Inflammatory Agents Cardioprotective NF-kappaB Inhibitor Renoprotective Tumor Necrosis Factor (TNF) Alpha Inhibitor Animal Study Tue, 18 Dec 2018 19:57:45 +0000 greenmedinfo 175985 at https://greenmedinfo.com A compound within Andrographis prevent diabetic nephropathy in an animal model. https://greenmedinfo.com/article/compound-within-andrographis-prevent-diabetic-nephropathy-animal-model PMID:  J Ethnopharmacol. 2010 Aug 30. Epub 2010 Aug 30. PMID: 20813180 Abstract Title:  Andrographolide and 14-deoxy-11,12-didehydroandrographolide from Andrographis paniculata attenuate high glucose-induced fibrosis and apoptosis in murine renal mesangeal cell lines. Abstract:  ETHNOPHARMACOLOGICAL RELEVANCE: Extracts of Andrographis paniculata Nees is used for various ethnomedical conditions including hyperglycemia and hypertension complications. AIM OF THE STUDY: The purpose of this study is to evaluate the anti-diabetic nephropathy effect of diterpene lactones andrographolide (AP1) and 14-deoxy-11,12-didehydroandrographolide (AP2) from A. paniculata. MATERIALS AND METHODS: MES-13, a SV40 transformed murine glomerular mesangial cell line, was cultured in high concentration of glucose to induce diabetic nephropathy phenotypes, which include secretion of extracellular matrix protein fibronectin, cytokine TGF-beta, states of oxidative stress, and apoptosis marker caspase-3. RESULTS: Our data suggest that addition of compounds AP1 or AP2 reduce the phenotypes indicating diabetic nephropathy in MES-13 cells. The compound AP2 showed potent activity than AP1 in the reduction of apoptosis marker caspase-3, fibrosis marker TGF-beta, and PAI-1. Furthermore, AP1 and AP2 do not have antioxidant ability in acellular environment, however addition of AP1 and AP2 reduced intracellular oxidative states in high glucose cultured MES-13 cells. CONCLUSION: This is the first report on anti-diabetic nephropathy effect of AP1 and AP2 in part due to regulation of intracellular signaling transduction, not mere clearance of reactive oxygen species. Thus, this study may useful for drug development or food supplement for diabetes and nephropathy from A. paniculata. https://greenmedinfo.com/article/compound-within-andrographis-prevent-diabetic-nephropathy-animal-model#comments Andrographis Diabetic Nephropathy Kidney Diseases Nephropathy: Diabetic Renoprotective Animal Study Mon, 13 Sep 2010 19:33:43 +0000 greenmedinfo 56351 at https://greenmedinfo.com A gastro-resistant peptide from Momordica charantia improves diabetic nephropathy. https://greenmedinfo.com/article/gastro-resistant-peptide-momordica-charantia-improves-diabetic-nephropathy PMID:  Food Funct. 2022 Feb 21 ;13(4):1822-1833. Epub 2022 Feb 21. PMID: 35083999 Abstract Title:  A gastro-resistant peptide fromimproves diabetic nephropathy in/miceits novel reno-protective and anti-inflammatory activities. Abstract:  Diabetic nephropathy (DN), a principal diabetic microvascular complication, is a chronic inflammatory immune disorder. A gastro-resistant peptide mcIRBP-9 fromhas shown modulation of blood glucose homeostasis in diabetic mice. Here we conducted a long-term experiment to evaluate the therapeutic effects and mechanisms of mcIRBP-9 on DN. Type 2 diabetic mice (/mice) were orally given mcIRBP-9 once daily for 12 consecutive weeks. The amelioration of DN was evaluated by renal function indexes, vascular leakage, and pathological lesions. Possible effective mechanisms of mcIRBP-9 on DN were analyzed by gene expression profiles. A pharmacokinetic study in rats was carried out to evaluate the oral bioavailability of mcIRBP-9. Our data showed that mcIRBP-9 was able to enter systemic circulation in rats after oral administration. In comparison with mock, long-term administration of mcIRBP-9 significantly decreased blood glucose (572.25± 1.55 mg dL213.50± 163.39 mg dL) and HbA1c levels (13.58± 0.30%8.23± 2.98%) and improved the survival rate (85.7%100%) in diabetic mice. mcIRBP-9 ameliorated DN by reducing renal vascular leakage and histopathological changes. mcIRBP-9 altered the pathways involved in inflammatory and immune responses, and the nuclear factor-κB played a central role in the regulation of mcIRBP-9-affected pathways. Moreover, mcIRBP-9 improved the inflammatory characteristic of DN in diabetic and non-diabetic mice. In conclusion, mcIRBP-9 displayed a novel anti-inflammatory activity and exhibited a reno-protective ability in addition tocontrolling the blood glucose and HbA1c levels. These findings suggested the role of mcIRBP-9 fromas a nutraceutical agent for diabetes and subsequent DN. <p><a href="https://greenmedinfo.com/article/gastro-resistant-peptide-momordica-charantia-improves-diabetic-nephropathy" target="_blank">read more</a></p> https://greenmedinfo.com/article/gastro-resistant-peptide-momordica-charantia-improves-diabetic-nephropathy#comments Bitter Melon Diabetic Nephropathy Anti-Inflammatory Agents NF-kappaB Inhibitor Renoprotective Animal Study Sun, 27 Mar 2022 21:24:42 +0000 greenmedinfo 255376 at https://greenmedinfo.com A natural product of acteoside ameliorate kidney injury in diabetes. https://greenmedinfo.com/article/natural-product-acteoside-ameliorate-kidney-injury-diabetes PMID:  Phytother Res. 2021 Sep ;35(9):5227-5240. Epub 2021 Jul 8. PMID: 34236110 Abstract Title:  A natural product of acteoside ameliorate kidney injury in diabetes db/db mice and HK-2 cells via regulating NADPH/oxidase-TGF-β/Smad signaling pathway. Abstract:  This study was designed to investigate the protective effects and mechanisms of acteoside on DKD in diabetes male db/db mice and high glucose-induced HK-2 cells. The diabetes db/db mice were divided randomly into model group, metformin group, irbesartan group, and acteoside group. We observed the natural product of acteoside exhibiting a significant effect in renal protection through analyzing of biochemical indicators and endogenous metabolites, histopathological observations, and western blotting. HK-2 cells subjected to high glucose were used in invitro experiments. The molecular mechanisms of them were investigated by RT-PCR and western blot. Acteoside prevents high glucose-induced HK-2 cells and diabetes db/db mice by inhibiting NADPH/oxidase-TGF-β/Smad signaling pathway. Acteoside regulated the disturbed metabolic pathway of lipid metabolism, glyoxylate and dicarboxylate metabolism, and arachidonic acid metabolism. We discovered the natural product of acteoside exhibiting a significant effect in renal protection. This study paved the way for further exploration of pathogenesis, early diagnosis, and development of a new therapeutic agent for DKD. <p><a href="https://greenmedinfo.com/article/natural-product-acteoside-ameliorate-kidney-injury-diabetes" target="_blank">read more</a></p> https://greenmedinfo.com/article/natural-product-acteoside-ameliorate-kidney-injury-diabetes#comments Diabetic Nephropathy Verbascoside Renoprotective Animal Study Sun, 06 Feb 2022 23:24:29 +0000 greenmedinfo 252939 at https://greenmedinfo.com A proteoglycan isolated from Ganoderma lucidum attenuates diabetic kidney disease. https://greenmedinfo.com/article/proteoglycan-isolated-ganoderma-lucidum-attenuates-diabetic-kidney-disease PMID:  J Ethnopharmacol. 2023 Jun 28 ;310:116405. Epub 2023 Mar 24. PMID: 36966849 Abstract Title:  A proteoglycan isolated from Ganoderma lucidum attenuates diabetic kidney disease by inhibiting oxidative stress-induced renal fibrosis both in vitro and in vivo. Abstract:  ETHNOPHARMACOLOGICAL RELEVANCE: Ganoderma lucidum (G. lucidum) was regarded as &quot;miraculous herb&quot; by the Chinese and recorded detailly in the &quot;Shen Nong Ben Cao Jing&quot; as a tonic to improve health and prolong life. A proteoglycan (namely, FYGL) was extracted from Ganoderma lucidum, which was a water-soluble hyperbranched proteoglycan, and was found to be able to protect pancreatic tissue against oxidative stress damage.AIM OF THE STUDY: Diabetic kidney disease (DKD) is a complication of diabetes, but the effective treatment is still lack. Chronic hyperglycemia in diabetic patients induce the accumulation of ROS, which injure the renal tissue and lead to the renal dysfunction. In this work, the efficacy and target mechanics of FYGL on diabetic renal function were investigated.MATERIALS AND METHODS: In the present study, the mechanism of the reno-protection of FYGL was analyzed on diabetic db/db mice and rat glomerular mesangial cells (HBZY-1) induced by high glucose (HG) with palmitate (PA) (HG/PA). In vitro, the levels of reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) were evaluated by commercial kits. the expressions of NOX1 and NOX4, phosphorylation of MAPK and NF-κB, and pro-fibrotic proteins were measured by Western blot. In vivo, diabetic db/db mice were gavaged with FYGL for 8 weeks, body weight and fasting blood glucose (FBG) were tested weekly. On 8th week, the serum, urine and renal tissue were collected for glucose tolerance test (OGTT), redox indicator (SOD, CAT, GSH and MDA), lipid metabolism (TC, TG, LDL and HDL), blood urea nitrogen (BUN), serum creatinine (Scr), uric acid (UA), 8-oxo-deoxyguanosine (8-OHdG), and the changes of histopathology and expression of collagen IV and AGEs.RESULTS: The results in vitro showed that FYGL significantly inhibited the HG/PA-induced HBZY-1 cells proliferation, ROS generation, MDA production, promoted SOD activity, and suppressed NOX1, NOX4, MAPK, NF-κB, and pro-fibrotic proteins expression. In addition, FYGL markedly alleviated blood glucose, antioxidant activity and lipid metabolism, improved renal functions, and relieved renal histopathological abnormalities, especially renal fibrosis.CONCLUSIONS: The antioxidant activity of FYGL can reduce ROS caused by diabetes and protect renal from oxidative stress-induced dysfunction, thereby improving renal function. This study shows that FYGL has the potential to treat diabetic kidney disease. <p><a href="https://greenmedinfo.com/article/proteoglycan-isolated-ganoderma-lucidum-attenuates-diabetic-kidney-disease" target="_blank">read more</a></p> https://greenmedinfo.com/article/proteoglycan-isolated-ganoderma-lucidum-attenuates-diabetic-kidney-disease#comments Diabetic Nephropathy Reishi Mushroom Anti-Fibrotic Antioxidants NF-kappaB Inhibitor Renoprotective Superoxide Dismutase Up-regulation Animal Study Fri, 23 Jun 2023 16:30:05 +0000 greenmedinfo 275482 at https://greenmedinfo.com A purple corn extract may be a potent therapeutic agent targeting abnormal angiogenesis in diabetic nephropathy leading to kidney failure. https://greenmedinfo.com/article/purple-corn-extract-may-be-potent-therapeutic-agent-targeting-abnormal-angioge PMID:  PLoS One. 2013 ;8(11):e79823. Epub 2013 Nov 20. PMID: 24278186 Abstract Title:  Anthocyanin-rich purple corn extract inhibit diabetes-associated glomerular angiogenesis. Abstract:  Diabetic nephropathy (DN) is one of the major diabetic complications and the leading cause of end-stage renal disease. Abnormal angiogenesis results in new vessels that are often immature and play a pathological role in DN, contributing to renal fibrosis and disrupting glomerular failure. Purple corn has been utilized as a daily food and exerts disease-preventive activities. This study was designed to investigate whether anthocyanin-rich purple corn extract (PCE) prevented glomerular angiogenesis under hyperglycemic conditions. Human endothelial cells were cultured in conditioned media of mesangial cells exposed to 33 mM high glucose (HG-HRMC-CM). PCE decreased endothelial expression of vascular endothelial growth factor (VEGF) and hypoxia inducible factor (HIF)-1α induced by HG-HRMC-CM. Additionally, PCE attenuated the induction of the endothelial marker of platelet endothelial cell adhesion molecule (PECAM)-1 and integrin β3 enhanced in HG-HRMC-CM. Endothelial tube formation promoted by HG-HRMC-CM was disrupted in the presence of PCE. In the in vivo study employing db/db mice treated with 10 mg/kg PCE for 8 weeks, PCE alleviated glomerular angiogenesis of diabetic kidneys by attenuating the induction of VEGF and HIF-1α. Oral administration of PCE retarded the endothelial proliferation in db/db mouse kidneys, evidenced by its inhibition of the induction of vascular endothelium-cadherin, PECAM-1 and Ki-67. PCE diminished the mesangial and endothelial induction of angiopoietin (Angpt) proteins under hypeglycemic conditions. The induction and activation of VEGF receptor 2 (VEGFR2) were dampened by treating PCE to db/db mice. These results demonstrate that PCE antagonized glomerular angiogenesis due to chronic hyperglycemia and diabetes through disturbing the Angpt-Tie-2 ligand-receptor system linked to renal VEGFR2 signaling pathway. Therefore, PCE may be a potent therapeutic agent targeting abnormal angiogenesis in DN leading to kidney failure. <p><a href="https://greenmedinfo.com/article/purple-corn-extract-may-be-potent-therapeutic-agent-targeting-abnormal-angioge" target="_blank">read more</a></p> https://greenmedinfo.com/article/purple-corn-extract-may-be-potent-therapeutic-agent-targeting-abnormal-angioge#comments Anthocyanins Corn: Purple Diabetic Nephropathy Hypoxia inducible factor-1 alpha (HIF-1α) inhibitor Renoprotective Vascular Endothelial Growth Factor Inhibitors Plant Extracts Animal Study Mon, 02 Oct 2017 21:43:33 +0000 greenmedinfo 153766 at https://greenmedinfo.com A systematic review and meta-analysis of Abelmoschus manihot for diabetic nephropathy. https://greenmedinfo.com/article/systematic-review-and-meta-analysis-abelmoschus-manihot-diabetic-nephropathy PMID:  Evid Based Complement Alternat Med. 2019 ;2019:9679234. Epub 2019 Apr 18. PMID: 31118973 Abstract Title:  for Diabetic Nephropathy: A Systematic Review and Meta-Analysis. Abstract:  Diabetic nephropathy (DN) is the leading cause of end-stage renal disease (ESRD). Many trials have shown thatcould further improve proteinuria and protect kidney function in patients with DN when added to a renin-angiotensin system (RAS) blocker. A systematic assessment of the efficacy and safety ofin DN is essential. Eight electronic databases were searched to identify eligible trials published from inception to December 2017. The Cochrane Risk of Bias Tool was used to evaluate the methodological quality of eligible studies. Seventy-two studies with 5,895 participants were identified. The methodological quality of included studies was generally low. The results indicated that, compared to a RAS blocker, combined treatment ofwith a RAS blocker was more effective for 24h urinary protein (24h UP) (mean difference [MD], -0.39 [95% confidence interval [CI], -0.46 to -0.33] g/d; P<p><a href="https://greenmedinfo.com/article/systematic-review-and-meta-analysis-abelmoschus-manihot-diabetic-nephropathy" target="_blank">read more</a></p> https://greenmedinfo.com/article/systematic-review-and-meta-analysis-abelmoschus-manihot-diabetic-nephropathy#comments Diabetic Nephropathy Okra Renoprotective Meta Analysis Review Sat, 21 Sep 2019 02:09:43 +0000 greenmedinfo 196887 at https://greenmedinfo.com A virgin coconut oil supplementary diet could be a therapy for kidney problems. https://greenmedinfo.com/article/virgin-coconut-oil-supplementary-diet-could-be-therapy-kidney-problems PMID:  Folia Med (Plovdiv). 2019 Jun 1 ;61(2):249-257. PMID: 31301655 Abstract Title:  Evaluation of Kidney Function Parameters in Diabetic Rats Following Virgin Coconut Oil Diet. Abstract:  BACKGROUND: Diabetes mellitus (DM) leads to disruption of kidney function parameters (KFPs) which are markers of kidney diseases, especially nephropathy. Virgin coconut oil (VCO) has been implicated in playing a significant role in DM management. However, its role on KFPs in DM is scarce.AIM: To evaluate the kidney function parameters following VCO diet in diabetic rats.MATERIALS AND METHODS: Twenty-five (25) male rats of 150 - 200 g were divided into 5 groups (n=5): Non-diabetic control (Group 1), diabetes control (Group 2), diabetes + metformin (Group 3), diabetes + 10% VCO (Group 4) and diabetes + 20% VCO (Group 5). Apart from Group 1, other groups were given intraperitone-ally 50 mg/kg of streptozotocin to induce diabetes mellitus. After 72 hours, fasting hyperglycaemia was confirmed by glucose oxidase method. All the rats were fed normal rat chow for 8 weeks. At 8th week, serum and urine samples were analysed for biochemical analysis. After 8 weeks, Group 1 and Group 2 continued to be fed on normal rat chow while other groups were treated with diets (VCO) or drug (metformin) for 4 weeks. At 12th week, urine samples were collected for biochemical analysis, the rats were sacrificed, and blood samples were collected by cardiac puncture.RESULTS: There were significant differences in some KFPs in diabetes control (Group 2) compared to other experimental groups. However, there was no significant difference in glomerular filtration rate (GFR) and serum sodium in all the groups.CONCLUSION: VCO supplementary diet improved the altered KFPs and could be a therapy for kidney problems. <p><a href="https://greenmedinfo.com/article/virgin-coconut-oil-supplementary-diet-could-be-therapy-kidney-problems" target="_blank">read more</a></p> https://greenmedinfo.com/article/virgin-coconut-oil-supplementary-diet-could-be-therapy-kidney-problems#comments Coconut Oil Diabetic Nephropathy Renoprotective Animal Study Tue, 23 Jul 2019 03:34:42 +0000 greenmedinfo 191735 at https://greenmedinfo.com Abelmoschus esculentus subfractions improved nephropathy with regulating dipeptidyl peptidase-4 and type 1 glucagon-like peptide receptor. https://greenmedinfo.com/article/abelmoschus-esculentus-subfractions-improved-nephropathy-regulating-dipeptidyl PMID:  J Food Drug Anal. 2019 Jan ;27(1):135-144. Epub 2018 Aug 14. PMID: 30648566 Abstract Title:  Abelmoschus esculentus subfractions improved nephropathy with regulating dipeptidyl peptidase-4 and type 1 glucagon-like peptide receptor in type 2 diabetic rats. Abstract:  Abelmoschus esculentus (AE) has been used in traditional medicine to ameliorate hyperglycemia, but its mucilage increased bioassay difficulties. We have obtained a series of AE subfractions. Among them F1 and F2 regulated dipeptidyl peptidase-4 (DPP-4) and type 1 glucagon-like peptide receptor (GLP-1R), the treatment targets for type 2 diabetes. F1, F2 and fraction residues (FR) showed advantage on different aspects, which attenuates insulin resistance and metabolic disorder in vivo, and prevents renal-tubular change in vitro. In the present study, using type 2 diabetes model induced by high fat diet (HFD) and streptozotocin (STZ), we aim to investigate whether AE prevent diabetic nephropathy by regulating the putative markers. The results showed that all the subfractions ameliorated albuminuria and renal hyperfiltration (measured by creatinine clearance rate; CCr) accompanied with diabetes, while F2 acted most promptly and consistently. Histologically AE reduced renal tubular change, fibrosis and fat deposition. F2 and FR exerted significant effects to decrease DPP-4 while increase GLP-1R. Although all the subfractions were effective to reduce oxidative stress, only F2 acted on kidneys specifically. In conclusion, we have demonstrated AE has benefits to regulate DPP-4 and GLP-1R, to reduce oxidative stress and renal fibrosis, with resultant to improve renalfunction and prevent diabetic renal damage. Taken together, F2 could be more promising to be developed as adjuvant for diabetic nephropathy. <p><a href="https://greenmedinfo.com/article/abelmoschus-esculentus-subfractions-improved-nephropathy-regulating-dipeptidyl" target="_blank">read more</a></p> https://greenmedinfo.com/article/abelmoschus-esculentus-subfractions-improved-nephropathy-regulating-dipeptidyl#comments Diabetes Mellitus: Type 2 Diabetic Nephropathy Okra Antioxidants Renoprotective Animal Study Sat, 21 Sep 2019 00:40:15 +0000 greenmedinfo 196874 at https://greenmedinfo.com