Leiomyoma https://greenmedinfo.com/taxonomy/term/2109/all en A flavonoid found within Licorice inhibits cell proliferation in human uterine leiomyoma (fibroid) cells and increases cell arrest. https://greenmedinfo.com/article/flavonoid-found-within-licorice-inhibits-cell-proliferation-human-uterine-leio PMID:  Reprod Sci. 2008 Jul;15(6):552-8. Epub 2008 May 16. PMID: 18487228 Abstract Title:  Induction of growth inhibition and apoptosis in human uterine leiomyoma cells by isoliquiritigenin. Abstract:  Isoliquiritigenin(ISL), a calchone flavonoid, has cancer-preventing properties and is often used in Chinese medicine. In the present study, the authors use ISL to determine its effect on cell proliferation and cell cycle progression in primary cultured human uterine leiomyoma cells. Cell viability and cell proliferation assays were conducted. Flow cytometry, annexin V apoptosis assay, and DNA fragmentation assay were performed to determine the effect of ISL on cell cycle and apoptosis. The expression of cell cycle regulatory-related proteins was evaluated by Western blot. The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by ISL treatment in a dose-dependent manner. Flow cytometry results showed that ISL induced subG1 and G2/M arrest. DNA fragmentation assay and annexin V apoptosis assays revealed apoptosis induction. ISL-induced growth inhibition in uterine leiomyoma cells was associated with increased p21(Cip1/) Waf1 expression in a p53-dependent manner. Activation of caspase-3 and downregulation of Bcl-2, cdk 2/4, and E2F, with a concomitant increase in dephosphorylation of Rb and poly-ADP-ribose polymerase cleavage, were observed. This study demonstrates that ISL inhibits cell proliferation by initiating apoptosis in human uterine leiomyoma cells coupled with increased cell cycle arrest. These results indicate that ISL could prove to be a promising chemopreventive and therapeutic agent against human uterine leiomyoma. https://greenmedinfo.com/article/flavonoid-found-within-licorice-inhibits-cell-proliferation-human-uterine-leio#comments Fibroid Tumor Fibroids: Uterine Leiomyoma Licorice In Vitro Study Mon, 29 Jun 2009 00:55:53 +0000 greenmedinfo 44926 at https://greenmedinfo.com Adlay hull extract fraction could be considered as a natural plant-based medicine in the prevention or treatment of uterine leiomyoma growth. https://greenmedinfo.com/article/adlay-hull-extract-fraction-could-be-considered-natural-plant-based-medicine-p PMID:  Molecules. 2019 Apr 19 ;24(8). Epub 2019 Apr 19. PMID: 31010220 Abstract Title:  Adlay (L. var.Stapf.) Hull Extract and Active Compounds Inhibit Proliferation of Primary Human Leiomyoma Cells and Protect against Sexual Hormone-Induced Mice Smooth Muscle Hyperproliferation. Abstract:  Uterine leiomyomas, also known as fibroids, are benign neoplasms of the uterus and have a high incidence rate in women of reproductive age. Hysterectomy or myomectomy is the initial treatment, but fibroids will recur if the patient is still exposed to similar risk factors. Therefore, developing new therapeutic strategies are urgently necessary. In this study, the anti-proliferation effects of each fraction of adlay seeds were evaluated in uterine leiomyomas, and we identified the potential phytochemical compounds. We found that the ethyl acetate fraction of adlay hull (AHE-ea) appeared to be highly efficient in the anti-proliferation of rat uterine leiomyoma ELT3 cells and primary human uterine leiomyoma (hUL) cells. The proliferation of primary human normal uterine smooth muscle (UtSMC) and normal uterine myometrial (hUM) cells were also suppressed by AHE-ea. Two phytosterols, stigmasterol andβ-sitosterol, were identified from AHE-ea fraction. Mice treated with AHE-ea and stigmasterol alone demonstrated reduced diethylstilbestrol/medroxyprogesterone 17-acetate (DES/MPA)-induced uterine myometrial hyperplasia, which is the critical step for the development of leiomyoma. Taken together, our results suggest that the AHE-ea fraction could be considered as a natural plant-based medicine in the prevention or treatment of uterine leiomyoma growth. <p><a href="https://greenmedinfo.com/article/adlay-hull-extract-fraction-could-be-considered-natural-plant-based-medicine-p" target="_blank">read more</a></p> https://greenmedinfo.com/article/adlay-hull-extract-fraction-could-be-considered-natural-plant-based-medicine-p#comments Adlay Leiomyoma Antiproliferative Plant Extracts In Vitro Study Wed, 10 Jul 2019 17:54:16 +0000 greenmedinfo 190922 at https://greenmedinfo.com Bisphenol A induces human uterine leiomyoma cell proliferation. https://greenmedinfo.com/article/bisphenol-induces-human-uterine-leiomyoma-cell-proliferation PMID:  Mol Cell Endocrinol. 2019 Jan 4. Epub 2019 Jan 4. PMID: 30615907 Abstract Title:  Bisphenol A induces human uterine leiomyoma cell proliferation through membrane-associated ERα36 via nongenomic signaling pathways. Abstract:  The role of ERα36 in regulating BPA&#039;s effects and its potential as a risk factor for human uterine fibroids were evaluated. BPA at low concentrations (10 μM - 10 μM) increased proliferation by facilitating progression of hormonally regulated, immortalized human uterine leiomyoma (ht-UtLM; fibroid) cells from G-Ginto S phase of the cell cycle; whereas, higher concentrations (100 μM-200 μM) decreased growth. BPA upregulated ERα36 gene and protein expression, and induced increased SOS1 and Grb2 protein expression, both of which are mediators of the MAPK/ERK1/2 pathway. EGFR (pEGFR), Ras, and MAPKwere phosphorylated with concurrent Src activation in ht-UtLM cells within 10 min of BPA exposure. BPA enhanced colocalization of phosphorylated Src (pSrc) to ERα36 and coimmunoprecipitation of pSrc with pEGFR. Silencing ERα36 with siERα36 abolished the above effects. BPA induced proliferation in ht-UtLM cells through membrane-associated ERα36 with activation of Src, EGFR, Ras and MAPK nongenomic signaling pathways. <p><a href="https://greenmedinfo.com/article/bisphenol-induces-human-uterine-leiomyoma-cell-proliferation" target="_blank">read more</a></p> https://greenmedinfo.com/article/bisphenol-induces-human-uterine-leiomyoma-cell-proliferation#comments Bisphenol Toxicity Leiomyoma Bisphenol A Increased Risk In Vitro Study Fri, 11 Jan 2019 19:30:36 +0000 greenmedinfo 177358 at https://greenmedinfo.com Curcumin inhibits leiomyoma (fibroid) cell proliferation and fibronection expression. https://greenmedinfo.com/article/curcumin-inhibits-leiomyoma-fibroid-cell-proliferation-and-fibronection-expres PMID:  Fertil Steril. 2009 May;91(5 Suppl):2177-84. Epub 2008 Jun 13. PMID: 18555241 Abstract Title:  Curcumin, a nutritional supplement with antineoplastic activity, enhances leiomyoma cell apoptosis and decreases fibronectin expression. Abstract:  OBJECTIVE: To determine if curcumin has an antiproliferative effect on leiomyoma cells via apoptosis induction and whether curcumin impacts extracellular matrix (ECM) production by assessing the fibronectin expression in leiomyoma cells treated with curcumin. DESIGN: Tissue culture study of immortalized human leiomyoma and patient-matched myometrial cells treated with curcumin. SETTING: University hospital. PATIENT(S): Immortalized leiomyoma and myometrial cells from patients with symptomatic leiomyomata. INTERVENTION(S): Tissue culture, followed by proliferation studies, RNA, and protein analysis. MAIN OUTCOME MEASURE(S): Cell proliferation, alteration in apoptotic signaling pathways. RESULT(S): Curcumin demonstrated an antiproliferative effect on leiomyoma cell lines (IC50 = 20 muM). Importantly, no statistically significant inhibition of growth was observed when patient-matched myometrial cells were exposed to equivalent concentrations of curcumin. Curcumin stimulated caspase-3 and caspase-9 expression while inhibiting extracellular signal-regulated kinase 1 (ERK 1), ERK 2, and nuclear factor kappa B (NF-kappaB), suggesting regulation of leiomyocyte apoptosis. Finally, curcumin inhibited expression of fibronectin in leiomyoma cells. CONCLUSION(S): Our findings demonstrate that curcumin inhibited uterine leiomyoma cell proliferation via regulation of the apoptotic pathway, and inhibited production of the ECM component fibronectin. Curcumin provides a novel direction for leiomyoma therapies. https://greenmedinfo.com/article/curcumin-inhibits-leiomyoma-fibroid-cell-proliferation-and-fibronection-expres#comments Curcumin Fibroid Tumor Leiomyoma In Vitro Study Mon, 29 Jun 2009 00:47:14 +0000 greenmedinfo 44925 at https://greenmedinfo.com Curcumin inhibits uterine leiomyoma cell proliferation. https://greenmedinfo.com/article/curcumin-inhibits-uterine-leiomyoma-cell-proliferation PMID:  Gynecol Endocrinol. 2010 Jul 30. Epub 2010 Jul 30. PMID: 20672906 Abstract Title:  Inhibitory effect of curcumin on uterine leiomyoma cell proliferation. Abstract:  Objective. Uterine leiomyomas are the most common gynaecological benign tumour and greatly affect reproductive health and wellbeing. They are the predominant indication for hysterectomy in premenopausal women. Curcumin, a well-known component of turmeric, has been reported to prevent various diseases such as cancer, diabetes and obesity. Previous study reported that curcumin represses the proliferation of several tumour cells. However, there has not been a precise characterisation of the curcumin-induced inhibition of uterine leiomyoma cells. In this study, we investigated the inhibitory effect of curcumin on leiomyoma cells proliferation. Study design. Eker rat-derived uterine leiomyoma cell lines (ELT-3 cells) were used. Cell proliferation was assessed by counting the number of cells and MTS assay. The activation of peroxisome proliferator-activated receptor-gamma (PPARgamma) was evaluated by luciferase assay. Results. We found that curcumin significantly inhibited ELT-3 cell proliferation. PPARgamma was expressed in ELT-3 cells and curcumin acted as a PPARgamma ligand. This inhibitory effect of curcumin was attenuated by the treatment of cells with PPARgamma antagonist. Conclusion. These experimental findings in vitro show that the inhibitory effect of curcumin on ELT-3 cell proliferation occurs through the activation of PPARgamma. Curcumin may be useful as an alternative therapy for uterine leiomyoma. https://greenmedinfo.com/article/curcumin-inhibits-uterine-leiomyoma-cell-proliferation#comments Curcumin Leiomyoma Anti-Proliferative In Vitro Study Thu, 10 Feb 2011 19:34:21 +0000 greenmedinfo 61080 at https://greenmedinfo.com Curcumol attenuates human uterine leiomyoma cell development. https://greenmedinfo.com/article/curcumol-attenuates-human-uterine-leiomyoma-cell-development PMID:  J Ethnopharmacol. 2023 Jun 28 ;310:116311. Epub 2023 Mar 7. PMID: 36894110 Abstract Title:  Curcumol, a major terpenoid from Curcumae Rhizoma, attenuates human uterine leiomyoma cell development via the p38MAPK/NF-κB pathway. Abstract:  ETHNOPHARMACOLOGICAL RELEVANCE: Uterine fibroids (UFs) are the most common benign tumors in women of reproductive age. Curcumae Rhizoma, the main essential oil component of which is curcumol, is widely used for the treatment of phymatosis in China due to its antitumor, anti-inflammatory, antithrombin, anti-tissue fibrosis and anti-oxygen pharmacological activities, but its potential for the treatment of UFs has not been evaluated.AIM OF THE STUDY: This study aimed to investigate the effects and mechanisms of curcumol intervention in human uterine leiomyoma cells (UMCs).MATERIALS AND METHODS: Putative targets of curcumol intervention in UFs were identified using network pharmacology strategies. Molecular docking was performed to assess the binding affinity of curcumol to core targets. A concentration gradient of curcumol (0, 50, 100, 200, 300, 400 and 500 μM) or RU-486 (mifepristone, 0, 10, 20, 40, 50, and 100 μM) was applied to UMCs, and cell viability was detected by the CCK-8 assay. Cell apoptosis and cell cycle were examined by flow cytometry, and cell migration was assessed by a wound-healing assay. Additionally, the mRNA and protein expression levels of critical pathway components were evaluated by RT‒PCR and western blotting. Finally, the actions of curcumol on different tumor cell lines were summarized.RESULTS: Network pharmacology predicted 62 genes with roles in the treatment of UFs with curcumol, and MAPK14 (p38MAPK) displayed a higher interaction degree. GO enrichment and KEGG analyses revealed that the core genes were abundantly enriched in the MAPK signaling pathway. The molecular binding of curcumol to core targets was relatively stable. In UMCs, 200, 300 and 400 μM curcumol treatment for 24 h decreased cell viability compared with that in the control group, and the greatest effect was detected at 48 h and maintained until 72 h. Curcumol arrested cells in the G0/G1 phase and subsequently suppressed mitosis, promoted early apoptosis and reduced the degree of wound healing in a concentration-dependent manner in UMCs. Furthermore, 200 μM curcumol decreased the mRNA and protein expression of p38MAPK, the mRNA expression of NF-κB, and the protein expression of Ki-67 and increased the mRNA and protein expression of Caspase 9. Curcumol (300 and 400 μM) decreased the mRNA and protein expression of p38MAPK, NF-κB, and Ki-67 and increased the protein expression of Caspase 9 in UMCs. Curcumol was demonstrated to treat tumor cell lines, including breast cancer, ovarian cancer, lung cancer, gastric cancer, liver cancer and nasopharyngeal carcinoma, but its effects on benign tumors have not yet been reported.CONCLUSION: Curcumol suppresses cell proliferation and cell migration while arresting the cell cycle in the G0/G1 phase and inducing cell apoptosis in UMCs via a mechanism related to p38MAPK/NF-κB pathway regulation. Curcumol may be a potential therapeutic and preventive agent in the treatment of benign tumors such as UFs. <p><a href="https://greenmedinfo.com/article/curcumol-attenuates-human-uterine-leiomyoma-cell-development" target="_blank">read more</a></p> https://greenmedinfo.com/article/curcumol-attenuates-human-uterine-leiomyoma-cell-development#comments Curcumol Leiomyoma Antiproliferative Apoptotic Cell cycle arrest NF-kappaB Inhibitor In Vitro Study Sun, 28 May 2023 14:44:21 +0000 greenmedinfo 272869 at https://greenmedinfo.com EGCG inhibits fibroid tumors in quail. https://greenmedinfo.com/article/egcg-inhibits-fibroid-tumors-quail PMID:  Nutr Res. 2008 Feb;28(2):92-7. PMID: 19083394 Abstract Title:  Chemoprevention of fibroid tumors by [-]-epigallocatechin-3-gallate in quail. Abstract:  Spontaneous leiomyomas of the oviduct are common tumors of the Japanese quail (Coturnix coturnix japonica), and fibroid tumors in the laying hen are similar to human fibroid tumors with respect to estrogen and progesterone receptors. This makes the quail a good animal model for screening potential agents for that aid in the prevention and treatment of human myoma uteri. We have previously reported a decreased incidence of leiomyomas in the oviduct of Japanese quail with antioxidant supplementation, for example, lycopene and soy isoflavones. Most of the health benefits associated with green tea consumption is attributed to EGCG, one of 4 major catechins found in green tea. This study investigated the effects of epigallocatechin-3-gallate supplementation on the development of leiomyomas in the oviduct of Japanese quail. We also measured serum and tissue levels of malondialdehyde and TNF-alpha. One hundred eighty quail (8 months old) were assigned to 3 treatment groups consisting of 6 replicates of 10 quail in each group. Animals were fed either a basal diet (control group) or the basal diet supplemented with 200 or 400 mg of EGCG/kg of diet. The animals were euthanized at the end of the 12-month study period, and the tumors were characterized. Epigallocatechin gallate supplementation significantly decreased the number of leiomyomas as compared with the controls (P = .001). The tumors in the EGCG fed birds were smaller than those found in the control birds (P = .001). Serum and liver malondialdehyde and TNF-alpha concentrations decreased (P = .001) with EGCG supplementation. The results indicate that dietary supplementation with EGCG reduces the incidence and size of spontaneously occurring leiomyoma of the oviduct in Japanese quail. Clinical trials should be conducted to investigate the efficacy of EGCG supplementation in the prevention and treatment of uterine leiomyoma in humans. https://greenmedinfo.com/article/egcg-inhibits-fibroid-tumors-quail#comments EGCG (Epigallocatechin gallate) Fibroid Tumor Fibroids: Uterine Leiomyoma Tumor Necrosis Factor (TNF) Alpha Inhibitor Animal Study Mon, 29 Jun 2009 01:14:37 +0000 greenmedinfo 44931 at https://greenmedinfo.com Euonymus alatus inhibits human uterine leiomyomal (fibroid) smooth muscle cells. https://greenmedinfo.com/article/euonymus-alatus-inhibits-human-uterine-leiomyomal-fibroid-smooth-muscle-cells PMID:  Int J Gynecol Cancer. 2006 Mar-Apr;16(2):843-8. PMID: 16681771 Abstract Title:  Euonymus alatus (Thunb.) Sieb induces apoptosis via mitochondrial pathway as prooxidant in human uterine leiomyomal smooth muscle cells. Abstract:  Euonymus alatus (Thunb.) Sieb (EA) is a traditional Korean herbal medicine, commonly used to treat tumors in Korea and China for centuries. Our earlier studies have indicated that EA exhibits antitumor properties, but its mechanism remains to be elucidated. In this study, we evaluated the molecular mechanism of EA in a human uterine leiomyomal smooth muscle cell (ULSMC) line. Water extracts of EA have been reported to not only function as antioxidants but also cause cytotoxic effect. We investigated the mechanism of EA-induced cytotoxicity in human ULSMC. When cells were cultured with 20-200 microg/mL EA for 6 h, caspase-3 was activated and then cells fell into apoptosis. Induction of apoptosis by EA was accompanied with increase of the cytosolic fractions of cytochrome c prior to the activation of caspase-3. The preculture with 5 mM of buthionine sulfoximine, an inhibitor of glutathione synthesis, facilitated EA-induced induction of apoptosis. The preculture with N-benzyloxycarbonyl-valyl-alanyl-aspartyl fluoromethylketone, a pan-caspase inhibitor, partially suppressed the induction of apoptosis. EA showed little toxic effect on peripheral blood mononuclear cells from healthy volunteers. These results indicate that EA acts as a prooxidant and induces caspase-3 activation and apoptosis via mitochondrial pathway. https://greenmedinfo.com/article/euonymus-alatus-inhibits-human-uterine-leiomyomal-fibroid-smooth-muscle-cells#comments Euonymus alatus (Burning Bush) Fibroid Tumor Fibroids: Uterine Leiomyoma In Vitro Study Mon, 29 Jun 2009 01:10:10 +0000 greenmedinfo 44930 at https://greenmedinfo.com Fibroid https://greenmedinfo.com/disease/fibroid <div class="field field-image"> <div class="field-items"> <div class="field-item odd"> <img class="imagefield imagefield-field_image" width="450" height="337" alt="" src="//cdn.greenmedinfo.com/sites/default/files/Fibroid.jpg?1476212758" /> </div> </div> </div> <div class="field field-copyright"> <div class="field-items"> <div class="field-item odd"> Copyright: &lt;a href=&#039;http://www.123rf.com/profile_rob3000&#039;&gt;rob3000 / 123RF Stock Photo&lt;/a&gt; </div> </div> </div> <fieldset class="fieldgroup group-facebook-like-info"><legend>Facebook Like Info</legend><div class="field field-facebook-total-count"> <div class="field-items"> <div class="field-item odd"> 0 </div> </div> </div> </fieldset> Fibroid Tumor Leiomyoma Tue, 14 Apr 2009 07:18:00 +0000 greenmedinfo 20023 at https://greenmedinfo.com Fibroid Uterus https://greenmedinfo.com/disease/fibroid-uterus <div class="field field-image"> <div class="field-items"> <div class="field-item odd"> <img class="imagefield imagefield-field_image" width="450" height="337" alt="" src="//cdn.greenmedinfo.com/sites/default/files/Fibroid_1.jpg?1476212895" /> </div> </div> </div> <div class="field field-copyright"> <div class="field-items"> <div class="field-item odd"> Copyright: &lt;a href=&#039;http://www.123rf.com/profile_rob3000&#039;&gt;rob3000 / 123RF Stock Photo&lt;/a&gt; </div> </div> </div> <fieldset class="fieldgroup group-facebook-like-info"><legend>Facebook Like Info</legend><div class="field field-facebook-total-count"> <div class="field-items"> <div class="field-item odd"> 0 </div> </div> </div> </fieldset> Fibroid Tumor Leiomyoma Tue, 14 Apr 2009 07:18:01 +0000 greenmedinfo 20025 at https://greenmedinfo.com Fibroma: Uterine https://greenmedinfo.com/disease/fibroma-uterine <div class="field field-image"> <div class="field-items"> <div class="field-item odd"> <img class="imagefield imagefield-field_image" width="450" height="337" alt="" src="//cdn.greenmedinfo.com/sites/default/files/Fibroid_3.jpg?1476213506" /> </div> </div> </div> <div class="field field-copyright"> <div class="field-items"> <div class="field-item odd"> Copyright: &lt;a href=&#039;http://www.123rf.com/profile_rob3000&#039;&gt;rob3000 / 123RF Stock Photo&lt;/a&gt; </div> </div> </div> <fieldset class="fieldgroup group-facebook-like-info"><legend>Facebook Like Info</legend><div class="field field-facebook-total-count"> <div class="field-items"> <div class="field-item odd"> 0 </div> </div> </div> </fieldset> Fibroid Tumor Leiomyoma Tue, 14 Apr 2009 07:18:01 +0000 greenmedinfo 20028 at https://greenmedinfo.com Fibromyoma https://greenmedinfo.com/disease/fibromyoma <div class="field field-image"> <div class="field-items"> <div class="field-item odd"> <img class="imagefield imagefield-field_image" width="450" height="337" alt="" src="//cdn.greenmedinfo.com/sites/default/files/Fibroid_4.jpg?1476215433" /> </div> </div> </div> <div class="field field-copyright"> <div class="field-items"> <div class="field-item odd"> Copyright: &lt;a href=&#039;http://www.123rf.com/profile_rob3000&#039;&gt;rob3000 / 123RF Stock Photo&lt;/a&gt; </div> </div> </div> <fieldset class="fieldgroup group-facebook-like-info"><legend>Facebook Like Info</legend><div class="field field-facebook-total-count"> <div class="field-items"> <div class="field-item odd"> 0 </div> </div> </div> </fieldset> Fibroid Tumor Leiomyoma Tue, 14 Apr 2009 07:18:02 +0000 greenmedinfo 20032 at https://greenmedinfo.com Fucoidan displays anti-proliferation and anti-fibrotic effects and exerts protective effects against uterine leiomyomas development. https://greenmedinfo.com/article/fucoidan-displays-anti-proliferation-and-anti-fibrotic-effects-and-exerts-prot PMID:  Cell Physiol Biochem. 2018 ;49(5):1970-1986. Epub 2018 Sep 20. PMID: 30235439 Abstract Title:  Fucoidan Inhibits the Proliferation of Leiomyoma Cells and Decreases Extracellular Matrix-Associated Protein Expression. Abstract:  BACKGROUND/AIMS: Uterine leiomyomas (ULs) are benign uterine tumors, and the most notable pathophysiologic feature of ULs is excessive accumulation of extracellular matrix (ECM). Fucoidan is a polysaccharide extracted from brown seaweeds that has a wide range of pharmacological properties, including anti-fibrotic effects. We aimed to study the effect of fucoidan on the growth of ULs activated by transforming growth factor beta (TGFβ).METHODS: We used ELT-3 (Eker rat leiomyoma tumor-derived cells) and HUtSMC (human uterine smooth muscle cells) as in vitro models. Cell viability was determined by the MTT assay. Cell colony formation was stained using crystal violet. The side population, cell cycle and apoptosis were analyzed using flow cytometry. Protein expression was assayed by western blot analysis. We also conducted in vivo experiments to confirm the inhibitory effects of fucoidan in nude mouse xenograft models. Tumor tissues were assayed by immunohistochemistry analysis.RESULTS: In our study, fucoidan caused a 50% growth inhibition using a dose of 0.5 mg/ml and decreased the stem cell activity after 48 h. In addition, fucoidan induced sub-G1 cell cycle arrest and apoptosis. Fucoidan down-regulated fibronectin, vimentin,α-SMA and the COL1A1 protein levels in TGFβ3-induced ELT-3 cells. In the cellular mechanism, fucoidan abrogated TGFβ3-induced levels of p-Smad2 and p-ERK1/2, as well as β-catenin translocation into the nucleus. Furthermore, fucoidan suppressed xenograft tumor growth in vivo.CONCLUSION: Fucoidan displays anti-proliferation and anti-fibrotic effects and exerts protective effects against ULs development. <p><a href="https://greenmedinfo.com/article/fucoidan-displays-anti-proliferation-and-anti-fibrotic-effects-and-exerts-prot" target="_blank">read more</a></p> https://greenmedinfo.com/article/fucoidan-displays-anti-proliferation-and-anti-fibrotic-effects-and-exerts-prot#comments Fucoidan Leiomyoma Antiproliferative Apoptotic In Vitro Study Thu, 22 Nov 2018 02:50:53 +0000 greenmedinfo 174339 at https://greenmedinfo.com Genistein suppresses estradiol-induced uterine leiomyoma cell proliferation. https://greenmedinfo.com/article/genistein-suppresses-estradiol-induced-uterine-leiomyoma-cell-proliferation PMID:  Life Sci. 2004 Dec 31;76(7):775-82. PMID: 19903033 Abstract Title:  Repressive effect of the phytoestrogen genistein on estradiol-induced uterine leiomyoma cell proliferation. Abstract:  OBJECTIVE: Uterine leiomyomas are the most common gynecological benign tumor and greatly affect reproductive health and well-being. They are the predominant indication for hysterectomy in premenopausal women. Current epidemiological study reported that soy products intake is inversely associated with diseases leading to hysterectomy. Genistein is a soy-derived phytoestrogen and its inhibitory effect on leiomyoma cell proliferation is reported. In this study, we investigated the siginificant inhibitory effect of genistein on estradiol (E(2))-induced leiomyoma cells proliferation. STUDY DESIGN: The Eker rat-derived uterine leiomyoma cell line ELT-3 cells were used. Cell proliferation was assessed by counting the number of cells. The expression of estrogen receptors and peroxisome proliferator-activated receptor-gamma (PPARgamma) was evaluated by Western blot analysis. RESULTS: PPARgamma was expressed in ELT-3 cells and genistein acted as PPARgamma ligand. This inhibitory effect of genistein was attenuated by the treatment of cells with PPARgamma antagonist bisphenol A diglycidyl ether (BADGE) or GW9662. CONCLUSION: These experimental findings in vitro show that the repressive effect of genistein on E(2)-induced ELT-3 cell proliferation is through the activation of PPARgamma. Genistein may be useful as an alternative therapy for leiomyoma. https://greenmedinfo.com/article/genistein-suppresses-estradiol-induced-uterine-leiomyoma-cell-proliferation#comments Estrogen Dominance Genistein Leiomyoma In Vitro Study Mon, 14 Dec 2009 23:30:55 +0000 greenmedinfo 48278 at https://greenmedinfo.com Genistein's inhibitory effect on leiomyoma (fibroids) cell proliferation are dependant on concentration. https://greenmedinfo.com/article/genisteins-inhibitory-effect-leiomyoma-fibroids-cell-proliferation-are-dependa PMID:  Hum Reprod. 2007 Oct;22(10):2623-31. Epub 2007 Aug 27. PMID: 17725991 Abstract Title:  Stimulatory and inhibitory effects of genistein on human uterine leiomyoma cell proliferation are influenced by the concentration. Abstract:  BACKGROUND: Due to dietary exposure of women to genistein, a soy-derived phytoestrogen, and the estrogen responsiveness of uterine leiomyomas &#039;fibroids&#039;, we evaluated the effects of genistein (0.001-50 microg/ml) on human uterine leiomyoma (UtLM) cells versus uterine smooth muscle cells (UtSMCs) in vitro. METHODS: Light microscopy was used to determine the effects of genistein on cell morphology. Proliferation was assessed using a colorimetric assay and proliferating cell nuclear antigen (PCNA) immunocytochemistry. Flow cytometry was used to quantitate cells in the S-phase and those undergoing apoptosis. A fluorometric assay and confocal microscopy were used to detect caspase-3 activity and apoptotic bodies, respectively. RESULTS: In UtLM cells, low concentrations (&lt; or = 1 microg/ml) of genistein stimulated proliferation, increased PCNA labeling and the percentage of cells in the S-phase, but this did not occur in UtSMCs. Higher concentrations (&gt; or = 10 microg/ml) of genistein adversely affected the morphology, significantly inhibited proliferation, decreased PCNA labeling, increased caspase-3 activity and induced apoptosis in both cell types. CONCLUSIONS: Genistein&#039;s effects are concentration-dependent in both cell lines. Lower concentrations elicit proliferative effects on UtLM cells only; whereas, higher concentrations alter morphology, inhibit proliferation, and increase caspase activity and apoptosis in both cell types, with the latter two effects being more extensive in UtSMCs. https://greenmedinfo.com/article/genisteins-inhibitory-effect-leiomyoma-fibroids-cell-proliferation-are-dependa#comments Fibroid Tumor Fibroids: Uterine Genistein Leiomyoma In Vitro Study Mon, 29 Jun 2009 01:07:34 +0000 greenmedinfo 44929 at https://greenmedinfo.com