Fibroid Tumor https://greenmedinfo.com/taxonomy/term/2110/all en A flavonoid found within Licorice inhibits cell proliferation in human uterine leiomyoma (fibroid) cells and increases cell arrest. https://greenmedinfo.com/article/flavonoid-found-within-licorice-inhibits-cell-proliferation-human-uterine-leio PMID:  Reprod Sci. 2008 Jul;15(6):552-8. Epub 2008 May 16. PMID: 18487228 Abstract Title:  Induction of growth inhibition and apoptosis in human uterine leiomyoma cells by isoliquiritigenin. Abstract:  Isoliquiritigenin(ISL), a calchone flavonoid, has cancer-preventing properties and is often used in Chinese medicine. In the present study, the authors use ISL to determine its effect on cell proliferation and cell cycle progression in primary cultured human uterine leiomyoma cells. Cell viability and cell proliferation assays were conducted. Flow cytometry, annexin V apoptosis assay, and DNA fragmentation assay were performed to determine the effect of ISL on cell cycle and apoptosis. The expression of cell cycle regulatory-related proteins was evaluated by Western blot. The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by ISL treatment in a dose-dependent manner. Flow cytometry results showed that ISL induced subG1 and G2/M arrest. DNA fragmentation assay and annexin V apoptosis assays revealed apoptosis induction. ISL-induced growth inhibition in uterine leiomyoma cells was associated with increased p21(Cip1/) Waf1 expression in a p53-dependent manner. Activation of caspase-3 and downregulation of Bcl-2, cdk 2/4, and E2F, with a concomitant increase in dephosphorylation of Rb and poly-ADP-ribose polymerase cleavage, were observed. This study demonstrates that ISL inhibits cell proliferation by initiating apoptosis in human uterine leiomyoma cells coupled with increased cell cycle arrest. These results indicate that ISL could prove to be a promising chemopreventive and therapeutic agent against human uterine leiomyoma. https://greenmedinfo.com/article/flavonoid-found-within-licorice-inhibits-cell-proliferation-human-uterine-leio#comments Fibroid Tumor Fibroids: Uterine Leiomyoma Licorice In Vitro Study Mon, 29 Jun 2009 00:55:53 +0000 greenmedinfo 44926 at https://greenmedinfo.com Curcumin inhibits leiomyoma (fibroid) cell proliferation and fibronection expression. https://greenmedinfo.com/article/curcumin-inhibits-leiomyoma-fibroid-cell-proliferation-and-fibronection-expres PMID:  Fertil Steril. 2009 May;91(5 Suppl):2177-84. Epub 2008 Jun 13. PMID: 18555241 Abstract Title:  Curcumin, a nutritional supplement with antineoplastic activity, enhances leiomyoma cell apoptosis and decreases fibronectin expression. Abstract:  OBJECTIVE: To determine if curcumin has an antiproliferative effect on leiomyoma cells via apoptosis induction and whether curcumin impacts extracellular matrix (ECM) production by assessing the fibronectin expression in leiomyoma cells treated with curcumin. DESIGN: Tissue culture study of immortalized human leiomyoma and patient-matched myometrial cells treated with curcumin. SETTING: University hospital. PATIENT(S): Immortalized leiomyoma and myometrial cells from patients with symptomatic leiomyomata. INTERVENTION(S): Tissue culture, followed by proliferation studies, RNA, and protein analysis. MAIN OUTCOME MEASURE(S): Cell proliferation, alteration in apoptotic signaling pathways. RESULT(S): Curcumin demonstrated an antiproliferative effect on leiomyoma cell lines (IC50 = 20 muM). Importantly, no statistically significant inhibition of growth was observed when patient-matched myometrial cells were exposed to equivalent concentrations of curcumin. Curcumin stimulated caspase-3 and caspase-9 expression while inhibiting extracellular signal-regulated kinase 1 (ERK 1), ERK 2, and nuclear factor kappa B (NF-kappaB), suggesting regulation of leiomyocyte apoptosis. Finally, curcumin inhibited expression of fibronectin in leiomyoma cells. CONCLUSION(S): Our findings demonstrate that curcumin inhibited uterine leiomyoma cell proliferation via regulation of the apoptotic pathway, and inhibited production of the ECM component fibronectin. Curcumin provides a novel direction for leiomyoma therapies. https://greenmedinfo.com/article/curcumin-inhibits-leiomyoma-fibroid-cell-proliferation-and-fibronection-expres#comments Curcumin Fibroid Tumor Leiomyoma In Vitro Study Mon, 29 Jun 2009 00:47:14 +0000 greenmedinfo 44925 at https://greenmedinfo.com EGCG inhibits fibroid tumors in quail. https://greenmedinfo.com/article/egcg-inhibits-fibroid-tumors-quail PMID:  Nutr Res. 2008 Feb;28(2):92-7. PMID: 19083394 Abstract Title:  Chemoprevention of fibroid tumors by [-]-epigallocatechin-3-gallate in quail. Abstract:  Spontaneous leiomyomas of the oviduct are common tumors of the Japanese quail (Coturnix coturnix japonica), and fibroid tumors in the laying hen are similar to human fibroid tumors with respect to estrogen and progesterone receptors. This makes the quail a good animal model for screening potential agents for that aid in the prevention and treatment of human myoma uteri. We have previously reported a decreased incidence of leiomyomas in the oviduct of Japanese quail with antioxidant supplementation, for example, lycopene and soy isoflavones. Most of the health benefits associated with green tea consumption is attributed to EGCG, one of 4 major catechins found in green tea. This study investigated the effects of epigallocatechin-3-gallate supplementation on the development of leiomyomas in the oviduct of Japanese quail. We also measured serum and tissue levels of malondialdehyde and TNF-alpha. One hundred eighty quail (8 months old) were assigned to 3 treatment groups consisting of 6 replicates of 10 quail in each group. Animals were fed either a basal diet (control group) or the basal diet supplemented with 200 or 400 mg of EGCG/kg of diet. The animals were euthanized at the end of the 12-month study period, and the tumors were characterized. Epigallocatechin gallate supplementation significantly decreased the number of leiomyomas as compared with the controls (P = .001). The tumors in the EGCG fed birds were smaller than those found in the control birds (P = .001). Serum and liver malondialdehyde and TNF-alpha concentrations decreased (P = .001) with EGCG supplementation. The results indicate that dietary supplementation with EGCG reduces the incidence and size of spontaneously occurring leiomyoma of the oviduct in Japanese quail. Clinical trials should be conducted to investigate the efficacy of EGCG supplementation in the prevention and treatment of uterine leiomyoma in humans. https://greenmedinfo.com/article/egcg-inhibits-fibroid-tumors-quail#comments EGCG (Epigallocatechin gallate) Fibroid Tumor Fibroids: Uterine Leiomyoma Tumor Necrosis Factor (TNF) Alpha Inhibitor Animal Study Mon, 29 Jun 2009 01:14:37 +0000 greenmedinfo 44931 at https://greenmedinfo.com Euonymus alatus inhibits human uterine leiomyomal (fibroid) smooth muscle cells. https://greenmedinfo.com/article/euonymus-alatus-inhibits-human-uterine-leiomyomal-fibroid-smooth-muscle-cells PMID:  Int J Gynecol Cancer. 2006 Mar-Apr;16(2):843-8. PMID: 16681771 Abstract Title:  Euonymus alatus (Thunb.) Sieb induces apoptosis via mitochondrial pathway as prooxidant in human uterine leiomyomal smooth muscle cells. Abstract:  Euonymus alatus (Thunb.) Sieb (EA) is a traditional Korean herbal medicine, commonly used to treat tumors in Korea and China for centuries. Our earlier studies have indicated that EA exhibits antitumor properties, but its mechanism remains to be elucidated. In this study, we evaluated the molecular mechanism of EA in a human uterine leiomyomal smooth muscle cell (ULSMC) line. Water extracts of EA have been reported to not only function as antioxidants but also cause cytotoxic effect. We investigated the mechanism of EA-induced cytotoxicity in human ULSMC. When cells were cultured with 20-200 microg/mL EA for 6 h, caspase-3 was activated and then cells fell into apoptosis. Induction of apoptosis by EA was accompanied with increase of the cytosolic fractions of cytochrome c prior to the activation of caspase-3. The preculture with 5 mM of buthionine sulfoximine, an inhibitor of glutathione synthesis, facilitated EA-induced induction of apoptosis. The preculture with N-benzyloxycarbonyl-valyl-alanyl-aspartyl fluoromethylketone, a pan-caspase inhibitor, partially suppressed the induction of apoptosis. EA showed little toxic effect on peripheral blood mononuclear cells from healthy volunteers. These results indicate that EA acts as a prooxidant and induces caspase-3 activation and apoptosis via mitochondrial pathway. https://greenmedinfo.com/article/euonymus-alatus-inhibits-human-uterine-leiomyomal-fibroid-smooth-muscle-cells#comments Euonymus alatus (Burning Bush) Fibroid Tumor Fibroids: Uterine Leiomyoma In Vitro Study Mon, 29 Jun 2009 01:10:10 +0000 greenmedinfo 44930 at https://greenmedinfo.com Fibroid https://greenmedinfo.com/disease/fibroid <div class="field field-image"> <div class="field-items"> <div class="field-item odd"> <img class="imagefield imagefield-field_image" width="450" height="337" alt="" src="//cdn.greenmedinfo.com/sites/default/files/Fibroid.jpg?1476212758" /> </div> </div> </div> <div class="field field-copyright"> <div class="field-items"> <div class="field-item odd"> Copyright: &lt;a href=&#039;http://www.123rf.com/profile_rob3000&#039;&gt;rob3000 / 123RF Stock Photo&lt;/a&gt; </div> </div> </div> <fieldset class="fieldgroup group-facebook-like-info"><legend>Facebook Like Info</legend><div class="field field-facebook-total-count"> <div class="field-items"> <div class="field-item odd"> 0 </div> </div> </div> </fieldset> Fibroid Tumor Leiomyoma Tue, 14 Apr 2009 07:18:00 +0000 greenmedinfo 20023 at https://greenmedinfo.com Fibroid Uterus https://greenmedinfo.com/disease/fibroid-uterus <div class="field field-image"> <div class="field-items"> <div class="field-item odd"> <img class="imagefield imagefield-field_image" width="450" height="337" alt="" src="//cdn.greenmedinfo.com/sites/default/files/Fibroid_1.jpg?1476212895" /> </div> </div> </div> <div class="field field-copyright"> <div class="field-items"> <div class="field-item odd"> Copyright: &lt;a href=&#039;http://www.123rf.com/profile_rob3000&#039;&gt;rob3000 / 123RF Stock Photo&lt;/a&gt; </div> </div> </div> <fieldset class="fieldgroup group-facebook-like-info"><legend>Facebook Like Info</legend><div class="field field-facebook-total-count"> <div class="field-items"> <div class="field-item odd"> 0 </div> </div> </div> </fieldset> Fibroid Tumor Leiomyoma Tue, 14 Apr 2009 07:18:01 +0000 greenmedinfo 20025 at https://greenmedinfo.com Fibroma: Uterine https://greenmedinfo.com/disease/fibroma-uterine <div class="field field-image"> <div class="field-items"> <div class="field-item odd"> <img class="imagefield imagefield-field_image" width="450" height="337" alt="" src="//cdn.greenmedinfo.com/sites/default/files/Fibroid_3.jpg?1476213506" /> </div> </div> </div> <div class="field field-copyright"> <div class="field-items"> <div class="field-item odd"> Copyright: &lt;a href=&#039;http://www.123rf.com/profile_rob3000&#039;&gt;rob3000 / 123RF Stock Photo&lt;/a&gt; </div> </div> </div> <fieldset class="fieldgroup group-facebook-like-info"><legend>Facebook Like Info</legend><div class="field field-facebook-total-count"> <div class="field-items"> <div class="field-item odd"> 0 </div> </div> </div> </fieldset> Fibroid Tumor Leiomyoma Tue, 14 Apr 2009 07:18:01 +0000 greenmedinfo 20028 at https://greenmedinfo.com Fibromyoma https://greenmedinfo.com/disease/fibromyoma <div class="field field-image"> <div class="field-items"> <div class="field-item odd"> <img class="imagefield imagefield-field_image" width="450" height="337" alt="" src="//cdn.greenmedinfo.com/sites/default/files/Fibroid_4.jpg?1476215433" /> </div> </div> </div> <div class="field field-copyright"> <div class="field-items"> <div class="field-item odd"> Copyright: &lt;a href=&#039;http://www.123rf.com/profile_rob3000&#039;&gt;rob3000 / 123RF Stock Photo&lt;/a&gt; </div> </div> </div> <fieldset class="fieldgroup group-facebook-like-info"><legend>Facebook Like Info</legend><div class="field field-facebook-total-count"> <div class="field-items"> <div class="field-item odd"> 0 </div> </div> </div> </fieldset> Fibroid Tumor Leiomyoma Tue, 14 Apr 2009 07:18:02 +0000 greenmedinfo 20032 at https://greenmedinfo.com Genistein's inhibitory effect on leiomyoma (fibroids) cell proliferation are dependant on concentration. https://greenmedinfo.com/article/genisteins-inhibitory-effect-leiomyoma-fibroids-cell-proliferation-are-dependa PMID:  Hum Reprod. 2007 Oct;22(10):2623-31. Epub 2007 Aug 27. PMID: 17725991 Abstract Title:  Stimulatory and inhibitory effects of genistein on human uterine leiomyoma cell proliferation are influenced by the concentration. Abstract:  BACKGROUND: Due to dietary exposure of women to genistein, a soy-derived phytoestrogen, and the estrogen responsiveness of uterine leiomyomas &#039;fibroids&#039;, we evaluated the effects of genistein (0.001-50 microg/ml) on human uterine leiomyoma (UtLM) cells versus uterine smooth muscle cells (UtSMCs) in vitro. METHODS: Light microscopy was used to determine the effects of genistein on cell morphology. Proliferation was assessed using a colorimetric assay and proliferating cell nuclear antigen (PCNA) immunocytochemistry. Flow cytometry was used to quantitate cells in the S-phase and those undergoing apoptosis. A fluorometric assay and confocal microscopy were used to detect caspase-3 activity and apoptotic bodies, respectively. RESULTS: In UtLM cells, low concentrations (&lt; or = 1 microg/ml) of genistein stimulated proliferation, increased PCNA labeling and the percentage of cells in the S-phase, but this did not occur in UtSMCs. Higher concentrations (&gt; or = 10 microg/ml) of genistein adversely affected the morphology, significantly inhibited proliferation, decreased PCNA labeling, increased caspase-3 activity and induced apoptosis in both cell types. CONCLUSIONS: Genistein&#039;s effects are concentration-dependent in both cell lines. Lower concentrations elicit proliferative effects on UtLM cells only; whereas, higher concentrations alter morphology, inhibit proliferation, and increase caspase activity and apoptosis in both cell types, with the latter two effects being more extensive in UtSMCs. https://greenmedinfo.com/article/genisteins-inhibitory-effect-leiomyoma-fibroids-cell-proliferation-are-dependa#comments Fibroid Tumor Fibroids: Uterine Genistein Leiomyoma In Vitro Study Mon, 29 Jun 2009 01:07:34 +0000 greenmedinfo 44929 at https://greenmedinfo.com Progesterone inhibits IGF-1 expression in human uterine leiomyoma (fibroid) cells. https://greenmedinfo.com/article/progesterone-inhibits-igf-1-expression-human-uterine-leiomyoma-fibroid-cells PMID:  Hum Reprod. 2004 Apr;19(4):815-21. Epub 2004 Jan 29. PMID: 15033949 Abstract Title:  Progesterone down-regulates insulin-like growth factor-I expression in cultured human uterine leiomyoma cells. Abstract:  BACKGROUND: Insulin-like growth factor-I (IGF-I) plays crucial roles in uterine leiomyoma cell growth through stimulating proliferation and inhibiting apoptosis. The present study was conducted to elucidate whether progesterone affects IGF-I and its receptor expression in cultured leiomyoma cells. METHODS: Isolated leiomyoma cells were subcultured in Phenol Red-free Dulbecco&#039;s modified Eagle&#039;s medium supplemented with 10% fetal bovine serum for 120 h and then stepped down to serum-free conditions for an additional 48 h in the presence or absence of 17beta-estradiol (E(2)) (10 ng/ml) or progesterone (100 ng/ml). IGF-I and its receptor mRNA and immunoreactive IGF-I in the cultured cells were assessed by quantitative RT-PCR with Southern blot analysis and by radioimmunoassay with Seppak C18 chromatography, respectively. The presence of estrogen receptor (ER) and progesterone receptor (PR) in cultured leiomyoma cells was immunocytochemically examined. RESULTS: Both treatment with progesterone alone and treatment with E(2) and progesterone combined significantly decreased IGF-I mRNA and protein expression in cultured leiomyoma cells compared with that in untreated cultures, but treatment with E(2) alone did not. IGF-I receptor mRNA expression in those cells was not affected by treatment with either E(2) or progesterone. Immunocytochemical analysis revealed that PR protein expression in cultured leiomyoma cells maintained in a serum-free condition for 48 h whereas ER protein expression in the cells remarkably decreased after 24 h culture under the serum-free condition. CONCLUSIONS: The present study provided evidence for the first time that progesterone down-regulates IGF-I mRNA and protein expression in cultured leiomyoma cells without affecting IGF-I receptor mRNA expression. NOTE: Progesterone, though derived from natural sources, e.g. mexican yam, is a powerful drug and should only be used under the careful guidance of a trained health professional. https://greenmedinfo.com/article/progesterone-inhibits-igf-1-expression-human-uterine-leiomyoma-fibroid-cells#comments Fibroid Tumor Fibroids: Uterine Leiomyoma Progesterone In Vitro Study Mon, 29 Jun 2009 01:25:23 +0000 greenmedinfo 44932 at https://greenmedinfo.com Scutellaria barbata reduces the over-expression of Bcl-2 protein. https://greenmedinfo.com/article/scutellaria-barbata-reduces-over-expression-bcl-2-protein PMID:  Phytother Res. 2008 May;22(5):583-90. PMID: 18444248 Abstract Title:  Antiproliferative effect of Scutellaria barbata D. Don. on cultured human uterine leiomyoma cells by down-regulation of the expression of Bcl-2 protein. Abstract:  Scutellaria barbata D. Don (Lamiaceae; SB) inhibited the growth of leiomyomal cells (LM). A time-dependent antiproliferative effect was noted when 10(-5) m buserelin, gonadotrophin-releasing hormone (GnRH) agonist or 20-40 microg/mL SB was added. The inhibition of cell growth decreased with the addition of the PKC activator (12-O-tetradecanoylphorbor-13-acetate; TPA) much as it did with the addition of SB, and the decreases in the viable cells caused by the addition of SB were reversed completely by pretreatment with a protein kinase C (PKC) inhibitor (calphostin C). The findings suggest that SB inhibits cell proliferation in cultured human uterine leiomyoma cells accompanied by PKC activation. Next, the study investigated the effect of SB on fetal development for toxicity. Pregnant Sprague-Dawley rats, from gestation day 6-15, were administered 20 g/L or 50 g/L SB in the drinking water and then killed on day 20. No maternal toxicity was observed, however, embryonic loss in the treatment groups was double that of the controls (p &lt; 0.05). No gross morphologic malformations were seen in the treated fetuses. Fetuses exposed to SB were found to be significantly heavier than the controls, an effect that was greater in female fetuses and was not correlated with increased placental size. The results suggest that the SB had no toxicity and that in utero exposure to SB resulted in increased early embryo loss with increased growth in surviving fetuses. On the other hand, Western blot analyses revealed that Bcl-2 protein of a 26 kDa was abundant in leiomyomal cells, but not in normal myometrial cells. The addition of progesterone (100 ng/mL) resulted in a striking increase in Bcl-2 protein expression in the cultured leiomyoma cells. However, the addition of SB (20 microg/mL) resulted in a significant reduction in Bcl-2 protein expression in the cells. The results indicated that human uterine leiomyomal cells express Bcl-2 protein and progesterone enhances its expression, however, SB reduces the expression of Bcl-2 protein in human uterine leiomyoma cells. https://greenmedinfo.com/article/scutellaria-barbata-reduces-over-expression-bcl-2-protein#comments Fibroid Tumor Fibroids: Uterine Leiomyoma Sculletaria barbata In Vitro Study Mon, 29 Jun 2009 00:59:33 +0000 greenmedinfo 44927 at https://greenmedinfo.com The Thyroid-Breast Cancer Connection https://greenmedinfo.com/blog/thyroid-breast-cancer-connection-1 <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2015<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="The Thyroid-Breast Cancer Connection" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/lrossi/images/thyroid_breast_cancer_greenmedinfo.png" style="width: 600px; height: 400px;" /></p> <p><span style="font-size:18px;"><em><strong>Iodine deficiency can lead to thyroid issues and eventually breast cancer.&nbsp;</strong></em></span></p> <p>In coaching hundreds of women on &nbsp;<strong><a href="http://www.breastcancerconqueror.com/7-essentials/" target="_blank">"The 7 Essentials System"</a></strong> for healing the body naturally, over half of them have "low thyroids" and many have been diagnosed with "thyroid cancer."</p><p><a href="https://greenmedinfo.com/blog/thyroid-breast-cancer-connection-1" target="_blank">read more</a></p> https://greenmedinfo.com/blog/thyroid-breast-cancer-connection-1#comments Breast Cancer FDB Fibroid Tumor Hypothyroidism Iodine Iodine Deficiency Thyroid Cancer bromide Cancer Flouride halides Iodine Breast Cancer bromide FDB Fibroid Tumor Flouride halides hypothyroidism Iodine Iodine Deficiency Thyroid Cancer Tue, 28 Apr 2015 18:54:10 +0000 DrV 117014 at https://greenmedinfo.com Vitamin D inhibits leiomyoma (fibroid) cell growth, in vitro. https://greenmedinfo.com/article/vitamin-d-inhibits-leiomyoma-fibroid-cell-growth-vitro PMID:  Fertil Steril. 2009 May;91(5):1919-25. Epub 2008 Apr 18. PMID: 18423458 Abstract Title:  Vitamin D inhibits myometrial and leiomyoma cell proliferation in vitro. Abstract:  OBJECTIVE: To determine the effect of 1,25(OH)(2)D(3) and 25(OH)D(3) vitamin D derivates on the growth of leiomyoma and myometrial cells in vitro. DESIGN: In vitro study. SETTING: Cell biology research laboratory. PATIENT(S): Six premenopausal women with uterine leiomyomas undergoing hysterectomy. INTERVENTION(S): Samples of leiomyomas and normal myometrial tissue were obtained, and paired cultures were established. MAIN OUTCOME MEASURE(S): A colorimetric crystal violet assay to determine the effect of 1,25(OH)(2)D(3) and 25(OH)D(3) on cell growth. RESULT(S): In both myometrial and leiomyoma cells, 0.1 nM physiologic level of 1,25(OH)(2)D(3) inhibited growth by 12% when compared with controls. The growth inhibition was concentration dependent; the highest concentration of 1,25(OH)(2)D(3) (100 nM) inhibited growth by 62% in both cell types. All the differences were statistically significant. A slight stimulation (&lt;4%) of cell proliferation was observed with the lowest 25(OH)(2)D(3) concentrations. When treated with either a 500 nM or 1000 nM concentration of the compound, the growth of both cell types fell to approximately 50% of that of the control cultures, and the level of inhibition with the latter concentration was statistically significant. CONCLUSION(S): Both myometrial and leiomyoma cell growth in vitro was effectively inhibited by 1,25(OH)(2)D(3). Vitamin D may play a role in the growth of uterine leiomyomas. https://greenmedinfo.com/article/vitamin-d-inhibits-leiomyoma-fibroid-cell-growth-vitro#comments Fibroid Tumor Fibroids: Uterine Leiomyoma Vitamin D In Vitro Study Mon, 29 Jun 2009 01:04:23 +0000 greenmedinfo 44928 at https://greenmedinfo.com