Lupus Nephritis https://greenmedinfo.com/taxonomy/term/3100/all en A soy diet accelerates renal damage in mice with lupus. https://greenmedinfo.com/article/soy-diet-accelerates-renal-damage-mice-lupus PMID:  J Biol Rhythms. 2010 Apr;25(2):113-22. PMID: 16039550 Abstract Title:  A soy diet accelerates renal damage in autoimmune MRL/Mp-lpr/lpr mice. Abstract:  Isoflavones, which are phytoestrogens present in large quantities in soy and soy-derived products, have estrogenic activity, inhibit protein tyrosine kinase, and exert other effects in the human body. Thus, the recent spread of soy consumption in Western populations emphasizes the need to more fully understand the potential effects in the body, especially in abnormal immune conditions. In the present study, the influence of a soy diet on lupus disease in MRL/Mp-lpr/lpr (MRL/lpr) mice was investigated. Weanling female MRL/lpr mice (4 weeks) were fed a soy diet (20% soybean protein and 5% soybean oil). The soy diet exacerbated renal damage; findings in this mouse strain included accelerated proteinuria, elevated serum creatinine concentrations, and reduced creatinine clearance. No effects were detected, however, in C3H/HeN mice, which have the same H-2(k) genetic background as MRL/lpr mice do. A tendency toward an increase in thymus weight and proliferation of T cells in spleen and B cells in lymph nodes were found at the age of 16 weeks. These findings indicate that a soy diet, in comparison with a casein diet, significantly exacerbates the clinical course of this autoimmune disease. Further research on the mechanism of this effect of soy-rich diets is needed, and isoflavone supplementation for systemic lupus erythematosus patients should be carefully reevaluated. https://greenmedinfo.com/article/soy-diet-accelerates-renal-damage-mice-lupus#comments Lupus Erythematosus: Systemic Lupus Nephritis Soy Animal Study Thu, 02 Jul 2009 18:27:54 +0000 greenmedinfo 44983 at https://greenmedinfo.com Anti-inflammatory and immunoregulatory effects of icariin and icaritin. https://greenmedinfo.com/article/anti-inflammatory-and-immunoregulatory-effects-icariin-and-icaritin PMID:  Biomed Pharmacother. 2022 Jul ;151:113180. Epub 2022 May 27. PMID: 35676785 Abstract Title:  Anti-inflammatory and immunoregulatory effects of icariin and icaritin. Abstract:  Inflammation and immunity dysregulation have received widespread attention in recent years due to their occurrence in the pathophysiology of many conditions. In this regard, several pharmacological studies have been conducted aiming to evaluate the potential anti-inflammatory and immunomodulatory effects of phytochemicals. Epimedium, a traditional Chinese medicine, is often used as a tonic, aphrodisiac, and anti-rheumatic agent. Icariin (ICA) is the main active ingredient of Epimedium and is, once ingested, mainly metabolized into Icaritin (ICT). Data from in vitro and in vivo studies suggested that ICA and its metabolite (ICT) regulated the functions and activation of immune cells, modulated the release of inflammatory factors, and restored aberrant signaling pathways. ICA and ICT were also involved in anti-inflammatory and immune responses in several diseases, including multiple sclerosis, asthma, atherosclerosis, lupus nephritis, inflammatory bowel diseases, rheumatoid arthritis, and cancer. Yet, data showed that ICA and ICT exhibited similar but not identical pharmacokinetic properties. Therefore, based on their higher solubility and bioavailability, as well as trends indicating that single-ingredient compounds offer broader and safer therapeutic capabilities, ICA and ICT delivery systems and treatment represent interesting avenues with promising clinical applications. In this study, we reviewed the anti-inflammatory and immunomodulatory mechanisms, as well as the pharmacokinetic properties of ICA and its metabolite ICT. <p><a href="https://greenmedinfo.com/article/anti-inflammatory-and-immunoregulatory-effects-icariin-and-icaritin" target="_blank">read more</a></p> https://greenmedinfo.com/article/anti-inflammatory-and-immunoregulatory-effects-icariin-and-icaritin#comments Asthma Atherosclerosis Icariin Inflammatory Bowel Diseases Lupus Nephritis Multiple Sclerosis Anti-Inflammatory Agents Immunomodulatory Review Wed, 25 Jan 2023 00:44:50 +0000 greenmedinfo 269986 at https://greenmedinfo.com Antrodia camphorata attenuates the progression of nephritis in systemic lupus erythematosus-prone mice. https://greenmedinfo.com/article/antrodia-camphorata-attenuates-progression-nephritis-systemic-lupus-erythemato PMID:  Evid Based Complement Alternat Med. 2008 Sep 2. PMID: 18955361 Abstract Title:  An Extract of Antrodia camphorata Mycelia Attenuates the Progression of Nephritis in Systemic Lupus Erythematosus-Prone NZB/W F1 Mice. Abstract:  Antrodia camphorata is used in folk medicine for the treatment of inflammation syndromes and liver-related diseases in Taiwan. The goal of this study was to evaluate the efficacy of the mycelial extract of A. camphorata (ACE) for the treatment of systemic lupus erythematosus (SLE) in SLE-prone NZB/W F1 mice. After antibodies against double-stranded DNA appeared in NZB/W mice, the mice were orally administered varying dosages of ACE (100, 200 and 400 mg kg(-1)) for 5 consecutive days per week for 12 weeks via gavage. To assess the efficacy of ACE, we measured SLE-associated biochemical and histopathological biomarkers levels of blood urine nitrogen (BUN), blood creatinine, urine protein and urine creatinine and thickness of the kidney glomerular basement membrane by staining with periodic acid-Schiff. Antroquinonol, an active component of ACE, was investigated for anti-inflammation activity in lipopolysaccharide-induced RAW 267.4 cells.ACE at 400 mg kg(-1) significantly suppressed urine protein and serum BUN levels and decreased the thickness of the kidney glomerular basement membrane. Antroquinonol significantly inhibited the production of tumor necrosis factor-alpha and interleukin-1beta by 75 and 78%, respectively. In conclusion, ACE reduced urine protein and creatinine levels and suppressed the thickening of the kidney glomerular basement membrane, suggesting that ACE protects the kidney from immunological damage resulting from autoimmune disease. https://greenmedinfo.com/article/antrodia-camphorata-attenuates-progression-nephritis-systemic-lupus-erythemato#comments Antrodia camphorata Lupus Erythematosus: Systemic Lupus Nephritis Animal Study Thu, 02 Jul 2009 17:17:11 +0000 greenmedinfo 44971 at https://greenmedinfo.com Astragalus amplifies the therapeutic effects of cyclophosphamide in the treatmeant of Lupus Nephritis. https://greenmedinfo.com/article/astragalus-amplifies-therapeutic-effects-cyclophosphamide-treatmeant-lupus-nep PMID:  Zhong Xi Yi Jie He Xue Bao. 2007 May;5(3):272-5. PMID: 17498486 Abstract Title:  [Effect of intravenous drip infusion of cyclophosphamide with high-dose Astragalus injection in treating lupus nephritis]. Abstract:  OBJECTIVE: To observe the effect of high-dose Astragalus injection and cyclophosphamide (CTX) on infection, urine protein and immune function of the patients with lupus nephritis. METHODS: Forty-three patients diagnosed as systemic lupus erythematosus (SLE) complicated by kidney damage and qi-deficiency syndrome were randomly divided into trial group (n=23) and control group (n=20). Patients in both groups were treated for 3 months. Intravenous drip infusion of 0.8 g CTX was administered to all patients once a month, while intravenous drip infusion of 20 ml Astragalus injection was only administered to patients in the trial group every day for 12 days in each month. RESULTS: The decrease of active clinical symptom score after the treatment in the trial group was greater than that in the control group (P&lt;0.05). The infection rates of the trial group and the control group were 4.35% and 25% respectively. The decrease of 24-hour urine protein and CD8, and the increase of red blood cell count and serum albumin in the trial group were greater than those in the control group, and there were significant differences between the two groups (P&lt;0.05). White blood cell count in the trial group was decreased less than that in the control group after the treatment (P&lt;0.05). CONCLUSION: High-dose Astragalus injection used together with CTX is more effective than CTX alone in decreasing infection rate and urine protein and improving immune function for patients with lupus nephritis. https://greenmedinfo.com/article/astragalus-amplifies-therapeutic-effects-cyclophosphamide-treatmeant-lupus-nep#comments Astragalus Lupus Nephritis Chemotherapeutic Agent: Cyclophosphamide Drug-Plant-Vitamin Synergies Animal Study Mon, 20 Apr 2009 06:18:22 +0000 greenmedinfo 43596 at https://greenmedinfo.com Celastrol alleviates murine lupus nephritis via inducting CD4+Foxp3+ regulatory T cells. https://greenmedinfo.com/article/celastrol-alleviates-murine-lupus-nephritis-inducting-cd4foxp3-regulatory-t-ce PMID:  Folia Histochem Cytobiol. 2022 Jul 6. Epub 2022 Jul 6. PMID: 35792673 Abstract Title:  Celastrol alleviates murine lupus nephritis via inducting CD4+Foxp3+ regulatory T cells. Abstract:  INTRODUCTION: Lupus nephritis (LN) is an autoimmune glomerulonephritis secondary to systemic lupus erythematosus. Commonly, immunosuppressive agents are required for treating LN. However, frequent use of conventional immunosuppressants may produce a variety of side effects. Hence, seeking alternative drugs for treating LN is very important. This report aims to Fig. out the immunoregulatory efficacy of celastrol (CLT) in LN.MATERIAL AND METHODS: A spontaneous in vivo model of LN was established in FasL-deficient B6/gld mice. ELISA was used for analyzing serum creatinine (Scr) and anti-dsDNA levels in mice. IHC staining, immunofluorescence and hematoxylin-eosin and PAS staining were applied to determine renal immunopathology and histology. Cytokine gene levels were assessed using RT-qPCR. CD4+Foxp3+ Treg frequency in murine kidneys, lymph nodes and spleens was determined using flow cytometry analysis.RESULTS: CLT treatment alleviated renal dysfunction and renal injury in LN-prone B6/gld mice. Moreover, CLT reduced CD3+ T cell infiltration and inhibited proinflammatory cytokine expression in renal tissues of B6/gld mice. Importantly, CLT enhanced CD4+FoxP3+ Treg frequency in kidneys, lymph nodes and spleens of B6/gld mice.CONCLUSIONS: CLT exerts therapeutic effects on murine LN by improving renal function and immunopathology and inducing CD4+FoxP3+ Tregs. <p><a href="https://greenmedinfo.com/article/celastrol-alleviates-murine-lupus-nephritis-inducting-cd4foxp3-regulatory-t-ce" target="_blank">read more</a></p> https://greenmedinfo.com/article/celastrol-alleviates-murine-lupus-nephritis-inducting-cd4foxp3-regulatory-t-ce#comments Celastrol Lupus Nephritis Immunomodulatory In Vitro Study Wed, 14 Sep 2022 22:17:26 +0000 greenmedinfo 263421 at https://greenmedinfo.com Citral alleviates the mouse ASLN model by inhibition of the activation signal of NLRP3 inflammasome and enhanced activation of Nrf2 antioxidant signaling. https://greenmedinfo.com/article/citral-alleviates-mouse-asln-model-inhibition-activation-signal-nlrp3-inflamma PMID:  Arthritis Res Ther. 2015 ;17:331. Epub 2015 Nov 19. PMID: 26584539 Abstract Title:  Citral alleviates an accelerated and severe lupus nephritis model by inhibiting the activation signal of NLRP3 inflammasome and enhancing Nrf2 activation. Abstract:  INTRODUCTION: Lupus nephritis (LN) is a major complication of systemic lupus erythematosus. NLRP3 inflammasome activation, reactive oxygen species (ROS) and mononuclear leukocyte infiltration in the kidney have been shown to provoke the acceleration and deterioration of LN, such as accelerated and severe LN (ASLN). Development of a novel therapeutic remedy based on these molecular events to prevent the progression of the disease is clinically warranted.METHODS: Citral (3,7-dimethyl-2,6-octadienal), a major active compound in a Chinese herbal medicine Litsea cubeba, was used to test its renoprotective effects in a lipopolysaccharide (LPS)-induced mouse ASLN model by examining NLRP3 inflammasome activation, ROS and COX-2 production as well as Nrf2 activation. The analysis of mechanisms of action of Citral also involved its effects on IL-1β secretion and signaling pathways of NLRP3 inflammasome in LPS-primed peritoneal macrophages or J774A macrophages.RESULTS: Attenuated proteinuria, renal function impairment, and renal histopathology, the latter including intrinsic cell proliferation, cellular crescents, neutrophil influx, fibrinoid necrosis in the glomerulus, and peri-glomerular infiltration of mononuclear leukocytes as well as glomerulonephritis activity score were observed in Citral-treated ASLN mice. In addition, Citral inhibited NLRP3 inflammasome activation and levels of ROS, NAD(P)H oxidase subunit p47(phox), or COX-2, and it enhanced the activation of nuclear factor E2-related factor 2 (Nrf2). In LPS-primed macrophages, Citral reduced ATP-induced IL-1β secretion and caspase-1 activation, but did not affect LPS-induced NLRP3 protein expression.CONCLUSION: Our data show that Citral alleviates the mouse ASLN model by inhibition of the activation signal, but not the priming signal, of NLRP3 inflammasome and enhanced activation of Nrf2 antioxidant signaling. https://greenmedinfo.com/article/citral-alleviates-mouse-asln-model-inhibition-activation-signal-nlrp3-inflamma#comments Citral Lupus Nephritis Antioxidants Nrf2 activation Renoprotective Animal Study Wed, 16 Mar 2016 23:06:44 +0000 greenmedinfo 124843 at https://greenmedinfo.com Cordyceps and artemisinin prevent the recurrence of lupus nephritis. https://greenmedinfo.com/article/cordyceps-and-artemisinin-prevent-recurrence-lupus-nephritis PMID:  Zhongguo Zhong Xi Yi Jie He Za Zhi. 2002 Mar;22(3):169-71. PMID: 12585097 Abstract Title:  [Study on effect of Cordyceps sinensis and artemisinin in preventing recurrence of lupus nephritis]. Abstract:  OBJECTIVE: To observe the effect of Cordyceps sinensis and artemisinin in preventing recurrence of lupus nephritis (LN). METHODS: Sixty-one LN patients, who had no activities by corticosterone and cyclophosphamide (CTX) impacting therapy were randomly divided into two groups. The 31 cases in the treated group were given Cordyceps powder 2-4 g/d before meal and artemisinin 0.6 g/d after meal in three portions orally taken for 3 years. The 30 patients in the control group were treated with tripterygiitotorum and/or Baoshenkang tablet. The consecutive observation lasted for 5 years to monitor the clinical manifestations of lupus and laboratory indexes including blood creatinine, creatinine clearance rate (CCr) and antinuclear antibodies (ANA). RESULTS: The therapeutic effect showed markedly effective in 26 cases (83.9%), effective in 4 (12.9%) and ineffective in 1 (3.2%) in the treated group, while in the control group, the corresponding numbers were 15 (50.0%), 8 (26.7%) and 7 (23.3%), the difference between the two groups in markedly effective rate was significant (P &lt; 0.01). In the treated group, C3 level was stabilized at above 1.21 +/- 0.20 g/L, which was over the normal range, CCr was unchanged as compared before and after treatment, which was significantly different from that in the control group. Moreover, the side-effects occurred in the treated group was less. CONCLUSION: Cordyceps and artemisinin could prevent the recurrence of LN and protect kidney function. https://greenmedinfo.com/article/cordyceps-and-artemisinin-prevent-recurrence-lupus-nephritis#comments Cordyceps sinensis Lupus Nephritis Human Study Mon, 20 Apr 2009 06:08:01 +0000 greenmedinfo 41265 at https://greenmedinfo.com Effectiveness and safety of tripterygium glycosides tablet for lupus nephritis. https://greenmedinfo.com/article/effectiveness-and-safety-tripterygium-glycosides-tablet-lupus-nephritis PMID:  J Tradit Chin Med. 2022 Oct ;42(5):671-680. PMID: 36083472 Abstract Title:  Effectiveness and safety of tripterygium glycosides tablet for lupus nephritis: a systematic review and Meta-analysis. Abstract:  OBJECTIVE: To investigate the effectiveness and safety of tripterygium glycosides (TG) tablet for the treatment of Lupus nephritis (LN).METHODS: Several databases were systematically searched including PubMed, Embase, Cochrane, Wiley, China National Knowledge Infrastructure Database, SinoMed and Wanfang Library till June 20, 2020. Revman5.3 was utilized to analyze the data according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Statement.RESULTS: In total, 8 randomized controlled trials involving 583 participants were identified. Meta-analyses showed that, compared with glucocorticoids (GC) alone, the combination with TG tablet provided a statistically significant improvement in total remission (TR) ( = 1.27, 95% : 1.08-1.50, = 0.004), complete remission (CR) ( = 1.61, 95% : 1.05-2.47, = 0.03) and C3 levels ( = 0.27, 95% : 0.14-0.39,<p><a href="https://greenmedinfo.com/article/effectiveness-and-safety-tripterygium-glycosides-tablet-lupus-nephritis" target="_blank">read more</a></p> https://greenmedinfo.com/article/effectiveness-and-safety-tripterygium-glycosides-tablet-lupus-nephritis#comments Lupus Nephritis Tripterygium wilfordii Meta Analysis Significant Treatment Outcome Review Wed, 14 Sep 2022 16:28:02 +0000 greenmedinfo 263394 at https://greenmedinfo.com Effects of Lactobacillus plantarum HFY15 on Lupus nephritis. https://greenmedinfo.com/article/effects-lactobacillus-plantarum-hfy15-lupus-nephritis PMID:  Drug Des Devel Ther. 2022 ;16:2851-2860. Epub 2022 Aug 26. PMID: 36051155 Abstract Title:  Effects ofHFY15 on Lupus Nephritis in Mice by Regulation of the TGF-β1 Signaling Pathway. Abstract:  Objective: In this study, theHFY15 (LP-HFY15) strain isolated from naturally fermented yak yogurt was investigated. An animal model of lupus nephritis was established by pristane to verify the interventional effect of LP-HFY15 on mouse lupus nephritis by regulating the transforming growth factor-β1 (TGF-β1) signaling pathway.Materials and Methods: Indexes in mouse serum and tissues were detected by kits, pathological changes in mouse kidney were observed by hematoxylin-eosin (H&amp;E) staining, and quantitative polymerase chain reaction (qPCR) was used to detect TGF-β 1-related expression in mouse kidney tissue, which further elucidated the mechanism of LP-HFY15.Results: LP-HFY15 decreased the elevation of urinary protein and the levels of interleukin-6 (IL-6), IL-12, tumor necrosis factor alpha (TNF-α), and interferon γ (IFN-γ) in serum and kidney tissue. LP-HFY15 also reduced serum creatinine (SCr), blood urea nitrogen (BUN), total cholesterol (TC), triglyceride (TG), and raised total protein (TP), and albumin (ALB) levels in mice with nephritis. In addition, LP-HFY15 inhibited the positiverate of double-stranded deoxyribonucleic acid (dsDNA) antibodies in mice with nephritis. The observation of H&amp;E sections showed that LP-HFY15 alleviated the glomerulus morphological incompleteness and inflammatory infiltration caused by nephritis. Further results showed that LP-HFY15 downregulated the mRNA expression of TGF-β1, vascular endothelial growth factor (VEGF), and nuclear factor kappa-B (NF-κB) in the kidneys of lupus nephritis mice, and the expression of inhibitor of NF-κB (IκB-α), copper/zinc superoxide dismutase (Cu/Zn-SOD), and manganese superoxide dismutase (Mn-SOD) was also upregulated.Conclusion: These results indicated that LP-HFY15 plays a significant role in experimental intervention for lupus nephritis. The effect of LP-HFY15 was positively correlated with its concentration, and the effect was similar to that of prednisone at 10CFU/kg. <p><a href="https://greenmedinfo.com/article/effects-lactobacillus-plantarum-hfy15-lupus-nephritis" target="_blank">read more</a></p> https://greenmedinfo.com/article/effects-lactobacillus-plantarum-hfy15-lupus-nephritis#comments Lactobacillus plantarum Lupus Nephritis Anti-Inflammatory Agents Interleukin-12 downregulation Interleukin-6 Downregulation Animal Study Fri, 16 Sep 2022 22:50:42 +0000 greenmedinfo 263529 at https://greenmedinfo.com Eicosapentaenoic acid (EPA) may exert beneficial effects on lupus nephritis by decreasing oxidative stress. https://greenmedinfo.com/article/eicosapentaenoic-acid-epa-may-exert-beneficial-effects-lupus-nephritis-decreas PMID:  Int J Obes (Lond). 2006 Aug;30(8):1298-307. Epub 2006 Feb 14. PMID: 16097442 Abstract Title:  Effects of eicosapentaenoic acids on oxidative stress and plasma fatty acid composition in patients with lupus nephritis. Abstract:  Eicosapentaenoic acid (EPA) is one of the major components of fish oil, which was reported to have antiatherogenic, anti-inflammatory and immune suppressive effects. In the present study, highly purified EPA was administered to patients with lupus nephritis and the effects of EPA on urinary 8-isoprostane, a reliable marker of oxidative stress, were investigated in these patients. Six outpatients (1 man and 5 women), with lupus nephritis diagnosed by renal biopsy, were entered in the study. We administered 1800 mg EPA ethyl-ester (purity &gt; 95%) daily and examined the urinary 8-isoprostane levels and plasma fatty acid composition before and 3 months after EPA treatment. The urinary 8-isoprostane levels were significantly decreased after the treatment compared with those before the treatment (from 530 +/- 113 pg/mg x Cr to 235 +/- 49 pg/mg x Cr, p = 0.02). The EPA levels in the plasma phospholipid (PL) fraction were significantly increased after the treatment (from 3.30 +/- 0.64 mol% to 8.01 +/- 0.47 mol%, p &lt; 0.001). Arachidonic acid (AA) levels in the plasma PL fraction were significantly decreased after the treatment (from 9.47 +/- 0.28 mol% to 7.33 +/- 0.43 mol%, p &lt; 0.001). The ratios of EPA to AA were significantly increased after the treatment (from 0.35 +/- 0.07 to 1.14 +/- 0.16, p &lt; 0.001). Thus, this preliminary study indicated that EPA might exert beneficial effects on lupus nephritis by decreasing the oxidative stress. https://greenmedinfo.com/article/eicosapentaenoic-acid-epa-may-exert-beneficial-effects-lupus-nephritis-decreas#comments EPA (Eicosapentaenoic Acid) Lupus Nephritis Human Study Thu, 02 Jul 2009 18:20:25 +0000 greenmedinfo 44982 at https://greenmedinfo.com Emodin may improve interstitial fibrosis, thus improving prognosis of lupus nephritis. https://greenmedinfo.com/article/emodin-may-improve-interstitial-fibrosis-thus-improving-prognosis-lupus-nephri PMID:  Zhongguo Zhong Xi Yi Jie He Za Zhi. 2000 Mar;20(3):196-8. PMID: 11789284 Abstract Title:  [Effect of emodin on fibroblasts in lupus nephritis]. Abstract:  OBJECTIVE: To observe the effects of emodin on human fibroblasts in culture of kidney in patients with lupus nephritis (LN). METHODS: Fibroblasts were isolated from culture of kidney of LN patients, and the effect of emodin on 3H-TdR incorporated rate of fibroblasts was observed. The apoptosis and c-myc gene expression were detected in the same time by flow cytometry. RESULTS: Emodin could inhibit the proliferation of fibroblasts, and promote the programmed cell death through upregulate c-myc gene expression in human renal fibroblasts. CONCLUSION: Emodin can inhibit proliferation and promote apoptosis of fibroblasts, which may be important in ameliorate interstitial fibrosis, thus improving prognosis of LN. https://greenmedinfo.com/article/emodin-may-improve-interstitial-fibrosis-thus-improving-prognosis-lupus-nephri#comments Emodin Lupus Nephritis Human Study Thu, 02 Jul 2009 20:21:00 +0000 greenmedinfo 44988 at https://greenmedinfo.com Fisetin reduces the senescent tubular epithelial cell burden and also inhibits proliferative fibroblasts in murine lupus nephritis. https://greenmedinfo.com/article/fisetin-reduces-senescent-tubular-epithelial-cell-burden-and-also-inhibits-pro PMID:  Front Immunol. 2022 ;13:960601. Epub 2022 Nov 17. PMID: 36466895 Abstract Title:  Fisetin reduces the senescent tubular epithelial cell burden and also inhibits proliferative fibroblasts in murine lupus nephritis. Abstract:  Systemic lupus erythematosus (SLE) is a chronic autoimmune inflammatory disease characterized by the involvement of multiple organs. Lupus nephritis (LN) is a major risk factor for overall morbidity and mortality in SLE patients. Hence, designing effective drugs is pivotal for treating individuals with LN. Fisetin plays a senolytic role by specifically eliminating senescent cells, inhibiting cell proliferation, and exerting anti-inflammatory, anti-oxidant, and anti-tumorigenic effects. However, limited research has been conducted on the utility and therapeutic mechanisms of fisetin in chronic inflammation. Similarly, whether the effects of fisetin depend on cell type remains unclear. In this study, we found that LN-prone MRL/lpr mice demonstrated accumulation of Ki-67-positive myofibroblasts and p15-positive senescent tubular epithelial cells (TECs) that highly expressed transforming growth factorβ(TGF-β). TGF-βstimulation induced senescence of NRK-52E renal TECs and proliferation of NRK-49F renal fibroblasts, suggesting that TGF-βpromotes senescence and proliferation in a cell type-dependent manner, which is inhibited by fisetin treatment. Furthermore, fisetin treatmentreduced the number of senescent TECs and myofibroblasts, which attenuated kidney fibrosis, reduced senescence-associated secretory phenotype (SASP) expression, and increased TEC proliferation. These data suggest that the effects of fisetin vary depending on the cell type and may have therapeutic effects in complex and diverse LN pathologies. <p><a href="https://greenmedinfo.com/article/fisetin-reduces-senescent-tubular-epithelial-cell-burden-and-also-inhibits-pro" target="_blank">read more</a></p> https://greenmedinfo.com/article/fisetin-reduces-senescent-tubular-epithelial-cell-burden-and-also-inhibits-pro#comments Fisetin Lupus Nephritis Anti-Fibrotic Antioxidants Renoprotective In Vitro Study Wed, 25 Jan 2023 22:27:25 +0000 greenmedinfo 270025 at https://greenmedinfo.com Fish oil supplementation and energy restriction protect against renal deterioration and oxidative damage in lupus-prone mice. https://greenmedinfo.com/article/fish-oil-supplementation-and-energy-restriction-protect-against-renal-deterior PMID:  Biochem Pharmacol. 2005 Sep 1;70(5):700-13. PMID: 16022752 Abstract Title:  Effects of energy restriction and fish oil supplementation on renal guanidino levels and antioxidant defences in aged lupus-prone B/W mice. Abstract:  Energy restriction (ER) and dietary fish oil (FO) are known to reduce the severity of glomerulonephritis and increase the lifespan of lupus-prone (NZB x NZW) F1 (B/W) mice. In the present study, mice were fed either ad libitum or energy-restricted (a 40 % lower energy intake than the diet ad libitum), semi-purified diets containing 5 % maize oil or 5 % fish oil supplementation. To estimate the renal damage associated with oxidative stress, the total amounts of reactive oxygen species (ROS), cyclooxygenase-derived ROS and levels of guanidino compounds were measured. Additionally, we assessed the putative action of ER and FO on several key antioxidant enzymes measured in the kidney post-mitochondrial fraction. Results showed that the age-related increase in creatinine level was significantly reduced by ER and FO in old mice. In contrast, arginine and guanidino acetic acid levels showed a decrease with age but were increased by ER and FO. The GSH:GSSG ratio showed a significant decrease with age, whereas ER and FO feeding prevented the decrease. The age-related decrease in antioxidant scavenging superoxide dismutase, catalase and glutathione peroxidase activities were all reversed by ER and FO. The moderately decreased glutathione reductase and glutathione-S-transferase activities with age were significantly increased by ER and FO. Furthermore, the increased total ROS and cyclooxygenase-derived ROS levels were effectively reduced by ER and FO. In conclusion, our data strongly indicate that ER and FO maintain antioxidant status and GSH:GSSG ratio, thereby protecting against renal deterioration from oxidative insults during ageing. https://greenmedinfo.com/article/fish-oil-supplementation-and-energy-restriction-protect-against-renal-deterior#comments Fish Oil Lupus Erythematosus: Systemic Lupus Nephritis Animal Study Thu, 02 Jul 2009 18:30:43 +0000 greenmedinfo 44984 at https://greenmedinfo.com Flaxseed supplementation appears to be renoprotective in lupus nephritis. https://greenmedinfo.com/article/flaxseed-supplementation-appears-be-renoprotective-lupus-nephritis PMID:  J Am Coll Nutr. 2001 Apr;20(2 Suppl):143-8. PMID: 11349937 Abstract Title:  Flaxseed in lupus nephritis: a two-year nonplacebo-controlled crossover study. Abstract:   OBJECTIVE: The objective of this study was to determine the renoprotective effects of ground flaxseed in patients with lupus nephritis. METHODS: Forty patients with lupus nephritis were asked to participate in a randomized crossover trial of flaxseed. Twenty-three agreed and were randomized to receive 30 grams of ground flaxseed daily or control (no placebo) for one year, followed by a twelve-week washout period and the reverse treatment for one year. At baseline and six month intervals, serum phospholipids, flaxseed sachet counts, serum creatinine, 12-hour urine albumin excretion and urine albumin to creatinine ratios, serum viscosity and plasma lipids were measured. RESULTS: There were eight drop-outs and of the 15 remaining subjects flaxseed sachet count and serum phospholipid levels indicated only nine were adherent to the flaxseed diet. Plasma lipids and serum viscosity were unaltered by the flaxseed supplementation whereas serum creatinine in the compliant patients during flaxseed administration declined from a mean of 0.97+/-0.31 mg/dL to a mean of 0.94+/-0.30 mg/dL and rose in the control phase to a mean of 1.03+/-0.28 mg/dL [p value &lt;0.08]. Of the fifteen patients who completed the study, similar changes were noted [p value &lt;0.1]. The nine compliant patients had lower serum creatinines at the end of the two-year study than the 17 patients who refused to participate [p&lt;0.05]. Microalbumin at baseline declined in both control and flaxseed time periods, but there was a trend for a greater decline during flaxseed administration [p&lt;0.2]. CONCLUSIONS: Flaxseed appears to be renoprotective in lupus nephritis, but this interpretation is affected by under powering due to poor adherence and potential Hawthorne effects. https://greenmedinfo.com/article/flaxseed-supplementation-appears-be-renoprotective-lupus-nephritis#comments Flaxseed Lupus Nephritis Human Study Thu, 02 Jul 2009 20:31:08 +0000 greenmedinfo 44990 at https://greenmedinfo.com Green tea powder inhibits the progression of lupus-like syndrome and prolongs survival in mice. https://greenmedinfo.com/article/green-tea-powder-inhibits-progression-lupus-syndrome-and-prolongs-survival-mic PMID:  In Vivo. 2003 Nov-Dec;17(6):545-52. PMID: 14758719 Abstract Title:  Inhibitory effects of autoimmune disease by green tea in MRL-Faslprcg/Faslprcg mice. Abstract:  To investigate whether green tea has inhibitory effects on the development of autoimmune disease (AID), one-month-old MRL-Faslprcg/Faslprcg mice were fed diets containing 2% green tea powder (GTP) for 3 months. At the end of GTP feeding, the weights of body, subcutaneous (s.c.) and intraperitoneal (i.p.) lymph nodes (LN), kidneys, spleen and intraperitoneal adipose tissue (IPAT), serological abnormalities and renal lesions were compared between GTP-fed and control mice. SCLN, IPLN, kidneys and IPAT weights in both sexes, spleen weight in males and body weight increase in males were significantly lower in GTP-fed mice. Particularly, LN hyperplasia and fatty accumulation were markedly reduced by GTP. Serum levels of anti-DNA antibodies and immune complexes (IC) were significantly lowered and proteinuria and blood urea nitrogen tended to be improved by GTP. The incidence of serious glomerulonephritis was significantly lower and nephric vasculitis was almost completely prevented in GTP-fed mice. Moreover, the survival of mice was significantly prolonged by GTP feeding for 6 months. These results indicate that the progression of lupus-like syndrome including glomerulonephritis was significantly delayed by reduced production of autoantibodies and IC in GTP-fed MRL-Faslprcg/Faslprcg mice, which led to the prolonged survival. https://greenmedinfo.com/article/green-tea-powder-inhibits-progression-lupus-syndrome-and-prolongs-survival-mic#comments Green Tea Lupus Erythematosus: Systemic Lupus Nephritis Animal Study Thu, 02 Jul 2009 20:15:53 +0000 greenmedinfo 44987 at https://greenmedinfo.com