Lymphoma: Dalton&#039;s https://greenmedinfo.com/taxonomy/term/3123/all en A moringa oleifera extract was shown to be comparable to the standard, 5-Fluorouracil treated animals. https://greenmedinfo.com/article/moringa-oleifera-extract-was-shown-be-comparable-standard-5-fluorouracil-treat PMID:  Indian J Exp Biol. 2015 Feb ;53(2):98-103. PMID: 25757240 Abstract Title:  Identification and characterization of a potent anticancer fraction from the leaf extracts of Moringa oleifera L. Abstract:  Anticancer potential of Moringa oleifera L. extracts have been well established. However, there are no reports on the isolated molecules/fractions from these extracts which are responsible for the anticancer/cytotoxic activity. Thus, in the present study, we explored the same. The n-hexane, chloroform, ethyl acetate, methanol extracts of the M. oleifera leaves and 15 fractions (F1 to F15) of ethyl acetate extract were evaluated for their in vitro and in vivo anticancer activity using Hep-2 cell lines and Dalton&#039;s lymphoma ascites model in mice, respectively. Among the tested samples, the F1 fraction showed potential cytotoxic effect in Hep-2 cell lines with a CTC50 value of 12.5± 0.5 μg/ml. In vivo studies with the doses 5 and 10 mg/kg, p.o. demonstrated significant reduction in body weight and increased the mean survival time compared to the control group. These results were also comparable to the standard, 5-Fluorouracil, treated animals. We have also successfully isolated and characterized the anticancer fraction, F1 from the leaves of M. oleifera L. https://greenmedinfo.com/article/moringa-oleifera-extract-was-shown-be-comparable-standard-5-fluorouracil-treat#comments Liver Cancer Lymphoma: Dalton's Moringa oleifera Antineoplastic Agents Cytotoxic Drug: 5-flourouracil Natural Substances Versus Drugs Plant Extracts Animal Study In Vitro Study Thu, 27 Aug 2015 02:20:30 +0000 greenmedinfo 120028 at https://greenmedinfo.com Moringa oleifera L. leaf extract induces cell cycle arrest and mitochondrial apoptosis in Dalton's Lymphoma. https://greenmedinfo.com/article/moringa-oleifera-l-leaf-extract-induces-cell-cycle-arrest-and-mitochondrial-ap PMID:  J Ethnopharmacol. 2022 Oct 25 ;302(Pt A):115849. Epub 2022 Oct 25. PMID: 36306933 Abstract Title:  Moringa oleifera L. leaf extract induces cell cycle arrest and mitochondrial apoptosis in Dalton&#039;s Lymphoma: An in vitro and in vivo study. Abstract:  ETHNOPHARMACOLOGICAL RELEVANCE: The present work is based on a wide spectrum of evidences available from scientific literature which reflects nutritional and medicinal values of natural products such as plants and their extracts. Moringa oleifera is one such popular plant species amidst indigenous tribal communities which is frequently used to treat ailments such as piles, sore throat, eye and ear infections and even poisonous bites of tropical fauna such as insects or snakes. Furthermore decoction of leaf and bark was used to cure fever and cough. Evidences further reveal that Moringa oleifera L. (Family Moringaceae), is widely distributed not only over the Indian sub-continent, but also over Philippines, Central America, Saudi Arabia and the Caribbean Islands and have been traditionally used to treat cancers since ancient times. However, therapeutic effects of Moringa oleifera on Non-Hodgkin Lymphoma (NHL) are yet to be established.AIM OF THE STUDY: The study aims to investigate the anti-cancer effects of Moringa oleifera leaf extract against murine NHL Non-Hodgkin cells in vitro and in vivo.MATERIAL AND METHODS: The pharmacologically active compounds of Moringa oleifera leaf extract were identified by GC-HRMS analysis. Tests of Moringa oleifera leaf extract&#039;s cytotoxicity against DL cells were carried out using the MTT assay. Chromatin condensation along with other morphological alterations were visualized through Fluorescence microscopy. Changes in the mitochondrial membrane potential (ΔΨm), the cell cycle, and apoptosis were analysed through flow cytometer. We tried to identify proteins involved in apoptosis and cell cycle through Western blotting using BALB/c mice as a model organism.RESULTS: GC-HRMS study revealed that a methanol based leaf extract of Moringa oleifera (MOML) comprises of a variety of bioactive chemicals. Our results indicate that MOML successfully reduced the proliferation of DL cells by loweringΔΨm, changing overall cell morphology. DL cells treated with MOML showed arrested cell cycle at the G2/M phase and substantially up-regulated the expression of p53 and p21. Elevated levels of Bax, Cyt-c, and Caspase-3 and lowered expression levels of Bcl-2 protein suggested induction of apoptosis. Mechanistically, the anticancer efficacy of MOML is attributed to MEK/ERK-mediated pathway inactivation in DL cells. It is also interesting to note that MOML-mediated inhibition of DL growth was accompanied by apoptosis induction and improvement in hematological parameters in DL-bearing mice.CONCLUSION: Our finding suggested that MOML induces apoptosis and abrogates the growth of Dalton&#039;s lymphoma both in vitro and in vivo. <p><a href="https://greenmedinfo.com/article/moringa-oleifera-l-leaf-extract-induces-cell-cycle-arrest-and-mitochondrial-ap" target="_blank">read more</a></p> https://greenmedinfo.com/article/moringa-oleifera-l-leaf-extract-induces-cell-cycle-arrest-and-mitochondrial-ap#comments Lymphoma: Dalton's Moringa oleifera Antiproliferative Cell cycle arrest Animal Study In Vitro Study Wed, 23 Nov 2022 00:30:50 +0000 greenmedinfo 266851 at https://greenmedinfo.com The hydroalcoholic extract of A. marmelos exhibits strong antitumor and antioxidant activities in Dalton's lymphoma-bearing mice. https://greenmedinfo.com/article/hydroalcoholic-extract-marmelos-exhibits-strong-antitumor-and-antioxidant-acti PMID:  Indian J Pharmacol. 2012 Mar ;44(2):225-9. PMID: 22529480 Abstract Title:  Antiproliferative and antioxidant activity of Aegle marmelos (Linn.) leaves in Dalton&#039;s Lymphoma Ascites transplanted mice. Abstract:  OBJECTIVE: The present investigation was performed to evaluate the antiproliferative and antioxidant activity of Aegle marmelos leaves in Dalton&#039;s Lymphoma Ascites (DLA)-bearing mice.MATERIALS AND METHODS: The DLA cells maintained in vivo in Swiss albino mice were used for developing ascitic tumor in mice by intraperitoneal transplantation. The standardized 50% ethanolic extract of A. marmelos leaves (AMEE) was administered intraperitoneally in dose levels 200 and 400 mg/kg, after 24 hours of tumor inoculation in mice for two weeks.RESULTS: The AMEE treatment significantly prevented (P<p><a href="https://greenmedinfo.com/article/hydroalcoholic-extract-marmelos-exhibits-strong-antitumor-and-antioxidant-acti" target="_blank">read more</a></p> https://greenmedinfo.com/article/hydroalcoholic-extract-marmelos-exhibits-strong-antitumor-and-antioxidant-acti#comments Bael Lymphoma: Dalton's Antioxidants Antiproliferative Animal Study Sat, 09 Mar 2019 01:45:28 +0000 greenmedinfo 181046 at https://greenmedinfo.com Z. officinale paste could be used as natural spice and a potent antitumour agent. https://greenmedinfo.com/article/z-officinale-paste-could-be-used-natural-spice-and-potent-antitumour-agent PMID:  Appl Biochem Biotechnol. 2016 Jul 19. Epub 2016 Aug 19. PMID: 27435276 Abstract Title:  Protective Effect of Zingiber officinale Against Dalton&#039;s Lymphoma Ascites Tumour by Regulating Inflammatory Mediator and Cytokines. Abstract:  The aim of the present investigation was to evaluate Zingiber officinale paste against Dalton&#039;s lymphoma ascites (DLA)-induced tumours in Swiss albino mice. Experimental animals received Z. officinale paste (low dose 100 mg/kg bw and high dose 500 mg/kg bw) orally for eight alternative days. Treatment with Z. officinale paste showed significant increase in haemoglobin level and decrease in aspartate amino transferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma glutamyl transferase(γ-GT) level. Z. officinale paste reduced the inflammatory mediators and cytokine levels, such as inducible nitric oxide (iNOS), tumour necrosis factor level (TNF-α) and interleukin-1β (IL-1β). Treatment with Z. officinale paste also significantly increased the antioxidant enzyme level, such assuperoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and glutathione transferase (GST), and decreased the lipid peroxidation. Treatment also increased the vitamin C and E levels in treated animals compared with the DLA-bearing host. Histopathological studies also confirmed the protective influence of Z. officinale paste against DLA. The present study suggested that Z. officinale paste could be used as natural spice and a potent antitumour agent. <p><a href="https://greenmedinfo.com/article/z-officinale-paste-could-be-used-natural-spice-and-potent-antitumour-agent" target="_blank">read more</a></p> https://greenmedinfo.com/article/z-officinale-paste-could-be-used-natural-spice-and-potent-antitumour-agent#comments Ginger Lymphoma: Dalton's Anti-Inflammatory Agents Antioxidants Interleukin-1 beta downregulation Tumor Necrosis Factor (TNF) Alpha Inhibitor In Vitro Study Thu, 03 Nov 2016 18:02:54 +0000 greenmedinfo 138784 at https://greenmedinfo.com