Squamous cell carcinoma https://greenmedinfo.com/taxonomy/term/50211/all en Isoalantolactone inhibits UM-SCC-10A cell growth via cell cycle arrest and apoptosis induction https://greenmedinfo.com/article/isoalantolactone-inhibits-um-scc-10a-cell-growth-cell-cycle-arrest-and-apoptos PMID:  PLoS One. 2013 ;8(9):e76000. Epub 2013 Sep 30. PMID: 24098753 Abstract Title:  Isoalantolactone inhibits UM-SCC-10A cell growth via cell cycle arrest and apoptosis induction. Abstract:  Isoalantolactone is a sesquiterpene lactone compound isolated from the roots of Inula helenium L. Previous studies have demonstrated that isoalantolactone possesses antifungal, anti-bacterial, anti-helminthic and anti-proliferative properties in a variety of cells, but there are no studies concerning its effects on head and neck squamous cell carcinoma (HNSCC). In the present study, an MTT assay demonstrated that isoalantolactone has anti-proliferative activity against the HNSCC cell line (UM-SCC-10A). Immunostaining identified that this compound induced UM-SCC-10A cell apoptosis but not necrosis. To explain the molecular mechanisms underlying its effects, flow cytometry and western blot analysis showed that the apoptosis was associated with cell cycle arrest during the G1 phase, up-regulation of p53 and p21, and down-regulation of cyclin D. Furthermore, our results revealed that induction of apoptosis through a mitochondrial pathway led to up-regulation of pro-apoptotic protein expression (Bax), down-regulation of anti-apoptotic protein expression (Bcl-2), mitochondrial release of cytochrome c (Cyto c), reduction of mitochondrial membrane potential (MMP) and activation of caspase-3 (Casp-3). Involvement of the caspase apoptosis pathway was confirmed using caspase inhibitor Z-VAD-FMK pretreatment. Together, our findings suggest that isoalantolactone induced caspase-dependent apoptosis via a mitochondrial pathway and was associated with cell cycle arrest in the G1 phase in UM-SCC-10A cells. Therefore, isoalantolactone may become a potential drug for treating HNSCC. <p><a href="https://greenmedinfo.com/article/isoalantolactone-inhibits-um-scc-10a-cell-growth-cell-cycle-arrest-and-apoptos" target="_blank">read more</a></p> https://greenmedinfo.com/article/isoalantolactone-inhibits-um-scc-10a-cell-growth-cell-cycle-arrest-and-apoptos#comments Elecampane Head and Neck Cancer Squamous cell carcinoma Antiproliferative Apoptotic Tumor Suppressor Protein p53 Upregulation In Vitro Study Sun, 03 Oct 2021 02:50:32 +0000 greenmedinfo 246708 at https://greenmedinfo.com Oridonin induces G2/M cell cycle arrest and apoptosis in human oral squamous cell carcinoma. https://greenmedinfo.com/article/oridonin-induces-g2m-cell-cycle-arrest-and-apoptosis-human-oral-squamous-cell- PMID:  Eur J Pharmacol. 2017 Nov 15 ;815:282-289. Epub 2017 Sep 19. PMID: 28935563 Abstract Title:  Oridonin induces G2/M cell cycle arrest and apoptosis in human oral squamous cell carcinoma. Abstract:  Oridonin, an active diterpeniod isolated from Rabdosia rubescens, has been reported for its anti-tumor activity on several cancers, however, its effect on oral squamous cell carcinoma (OSCC) remains unclear. In this study, we demonstrated for the first time that oridonin inhibited the growth of OSCC cells both in vitro and in vivo. Oridonin decreased the proliferation and clonal formation of cultured OSCC cells in a dose-dependent manner. Further study indicated that oridonin induced G2/M phase arrest in OSCC cells, which was associated with the downregulation of proteins related to G2/M transition including cdc25C, cdc2 and cyclin B1, as well as the upregulation of p53 and phosphorylated-cdc2. In addition, we discovered that oridonin induced OSCC cell apoptosis by activating the intrinsic apoptotic pathway, which was indicated by the increased expression of cleaved-caspase 3, cleaved-caspase 9 and proapoptotic protein Bax and reduced expression of caspase 9 and antiapoptotic protein Bcl-xl. Finally, oridonin suppressed the growth of OSCC in an xenograft mouse model. Immunohistochemical analysis showed a reduction of cyclin B1-positive cancer cells and an increase of TUNEL-positive cancer cells in oridonin-treated mice. Therefore, oridonin may be a potentially effective agent for the treatment of OSCC in future. <p><a href="https://greenmedinfo.com/article/oridonin-induces-g2m-cell-cycle-arrest-and-apoptosis-human-oral-squamous-cell-" target="_blank">read more</a></p> https://greenmedinfo.com/article/oridonin-induces-g2m-cell-cycle-arrest-and-apoptosis-human-oral-squamous-cell-#comments Oral Cancer Oridonin Squamous cell carcinoma Antiproliferative Apoptotic Cell cycle arrest In Vitro Study Tue, 01 Sep 2020 16:03:23 +0000 greenmedinfo 226134 at https://greenmedinfo.com Resveratrol and oxyresveratrol inhibit the expression of cancer stem cell markers and might target cancer stem cells in a hypoxia-associated tumor. https://greenmedinfo.com/article/resveratrol-and-oxyresveratrol-inhibit-expression-cancer-stem-cell-markers-and PMID:  J Oral Biol Craniofac Res. 2022 Mar-Apr;12(2):253-257. Epub 2022 Mar 16. PMID: 35313655 Abstract Title:  Effect of resveratrol and oxyresveratrol on deferoxamine-induced cancer stem cell marker expression in human head and neck squamous cell carcinoma. Abstract:  Objectives: Resveratrol and oxyresveratrol, a resveratrol derivative, possess various pharmacological activities, including anti-cancer activities. Because cancer stem cells play an important role in cancer recurrence, the aims of this study were to investigate whether resveratrol or oxyresveratrol can inhibit the expression of cancer stem cell markers under hypoxia.Materials and methods: Deferoxamine was used to mimic the hypoxic condition. The mRNA expression of cancer stem cell markers was analyzed by Real-time PCR. Flow cytometry was used to determine the number ofandcells.Results: Deferoxamine dose-dependently induced the expression of cancer stem cell markers;,,,and. The induction of these cancer stem cells markers was inhibited when the cells were treated with either resveratrol or oxyresveratrol. Moreover, we found that resveratrol also reduced the number ofandcells after deferoxamine treatment.Conclusions: Resveratrol and oxyresveratrol inhibit the expression of cancer stem cell markers and might target cancer stem cells in a hypoxia-associated tumor. <p><a href="https://greenmedinfo.com/article/resveratrol-and-oxyresveratrol-inhibit-expression-cancer-stem-cell-markers-and" target="_blank">read more</a></p> https://greenmedinfo.com/article/resveratrol-and-oxyresveratrol-inhibit-expression-cancer-stem-cell-markers-and#comments Head and Neck Cancer Resveratrol Squamous cell carcinoma Antiproliferative Cancer Stem Cells In Vitro Study Sun, 03 Apr 2022 19:23:50 +0000 greenmedinfo 255718 at https://greenmedinfo.com "Capsaicin-induced apoptosis of FaDu human pharyngeal squamous carcinoma cells." https://greenmedinfo.com/article/capsaicin-induced-apoptosis-fadu-human-pharyngeal-squamous-carcinoma-cells PMID:  Yonsei Med J. 2012 Jul 1 ;53(4):834-41. PMID: 22665354 Abstract Title:  Capsaicin-induced apoptosis of FaDu human pharyngeal squamous carcinoma cells. Abstract:  PURPOSE: To investigate the anti-tumor effect of capsaicin on human pharyngeal squamous carcinoma cells (FaDu).MATERIALS AND METHODS: The expression of apoptosis/cell cycle-related proteins (or genes) was examined by reverse transcriptase- polymerase chain reaction, western blotting and ELISA methods, while the apoptotic cell population, cell morphology and DNA fragmentation levels were assessed using flow cytometry, fluorescence microscopy and agarose gel electrophoresis.RESULTS: Capsaicin was found to inhibit the growth and proliferation of FaDu cells in a dose- and time-dependent manner. Apoptotic cell death was confirmed by observing increases in nuclear condensation, nuclear DNA fragmentation and sub-G1 DNA content. The observed increase in cytosolic cytochrome c, activation of caspase 3 and PARP (p85) levels following capsaicin treatment indicated that the apoptotic response was mitochondrial pathway-dependent. Gene/protein expression analysis of Bcl-2, Bad and Bax further revealed decreased anti-apoptotic Bcl-2 protein and increased pro-apoptotic Bad/Bax expression. Furthermore, capsaicin suppressed the cell cycle progression at the G1/S phase in FaDu cells by decreasing the expression of the regulators of cyclin B1 and D1, as well as cyclin-dependent protein kinases cdk-1, cdk-2 and cdk-4.CONCLUSION: Our current data show that capsaicin induces apoptosis in FaDu cells and this response is associated with mitochondrial pathways, possibly by mediating cell cycle arrest at G1/S. https://greenmedinfo.com/article/capsaicin-induced-apoptosis-fadu-human-pharyngeal-squamous-carcinoma-cells#comments Capsaicin Nasopharyngeal Cancer Squamous cell carcinoma Antiproliferative Apoptotic Bcl-2 protein down-regulation Cell cycle arrest In Vitro Study Wed, 19 Dec 2012 22:29:48 +0000 greenmedinfo 87302 at https://greenmedinfo.com "Lung cancer: is the increasing incidence due to radioactive polonium in cigarettes?" https://greenmedinfo.com/article/lung-cancer-increasing-incidence-due-radioactive-polonium-cigarettes PMID:  South Med J. 1986 Feb ;79(2):145-50. PMID: 3003925 Abstract Title:  Lung cancer: is the increasing incidence due to radioactive polonium in cigarettes? Abstract:  This paper presents clinical, experimental, and epidemiologic evidence to help explain the rapidly increasing incidence of primary lung cancer, with recently observed reversal in leading cell type from squamous cell to adenocarcinoma. It postulates that this may be due to changes in modern cigarettes, with or without filters, which allow inhalation of increased amounts of radioactive lead and polonium and decreased amounts of benzopyrene. This hypothesis is based upon measurements of increased concentrations of radioactive polonium in the lungs of cigarette smokers, in modern tobaccos grown since 1950, and in high-phosphate fertilizers used for tobacco farming in industrialized countries. Critical support for this thesis is based upon experimental animal studies in which lung cancers that resemble adenocarcinomas are induced with as little as 15 rads of radioactive polonium, equal to one fifth the dosage inhaled by cigarette smokers who average two packs a day during a 25-year period. https://greenmedinfo.com/article/lung-cancer-increasing-incidence-due-radioactive-polonium-cigarettes#comments Lung Cancer Smoking Squamous cell carcinoma Polonium Review Fri, 23 Mar 2012 03:32:45 +0000 greenmedinfo 73531 at https://greenmedinfo.com "Polonium-210 or lead-210 present in cigarette smoke may be a significant causative factor in human lung cancer." https://greenmedinfo.com/article/polonium-210-or-lead-210-present-cigarette-smoke-may-be-significant-causative- PMID:  Science. 1975 May 16 ;188(4189):737-8. PMID: 1124396 Abstract Title:  Lung cancer induced in hamsters by low doses of alpha radiation from polonium-210. Abstract:  Lung cancers have been induced in 9 to 53 percent of hamsters given multiple intratracheal instillations of polonium-210 in amounts yielding lifetime exposures of 15 to 300 rads to the lungs. Cigarette smokers have previously been estimated to receive 20 rads to areas of the bronchial epithelium from deposited polonium-210. This finding thus supports the hypothesis that alpha radiation resulting from the polonium-210 or lead-210 present in cigarette smoke may be a significant causative factor in human lung cancer. https://greenmedinfo.com/article/polonium-210-or-lead-210-present-cigarette-smoke-may-be-significant-causative-#comments Lung Cancer Smoking Squamous cell carcinoma Lead-210 Polonium Review Fri, 23 Mar 2012 03:34:21 +0000 greenmedinfo 73533 at https://greenmedinfo.com 3,3'-diindolylmethane induces G2/M arrest and apoptosis in oral squamous cell carcinoma. https://greenmedinfo.com/article/33-diindolylmethane-induces-g2m-arrest-and-apoptosis-oral-squamous-cell-carcin PMID:  Chem Biol Interact. 2012 Feb 5 ;195(3):224-30. Epub 2012 Jan 24. PMID: 22290291 Abstract Title:  The dietary phytochemical 3,3&#039;-diindolylmethane induces G2/M arrest and apoptosis in oral squamous cell carcinoma by modulating Akt-NF-κB, MAPK, and p53 signaling. Abstract:  In light of the growing incidence of oral cancer in Taiwan, this study is aimed at investigating the antitumor activity of 3,3&#039;-diindolylmethane (DIM), an active metabolite of the phytochemical indole-3-carbinol (I3C), in oral squamous cell carcinoma (OSCC). DIM exhibited substantially higher antiproliferative potency than I3C in three OSCC cell lines with IC(50) values in SCC2095, SCC9, and SCC15 cells, respectively, of 22 versus 168μM, 25 versus 176μM, and 29versus 300μM. Flow cytometric analysis and Comet assay indicated that DIM suppressed the viability of SCC2095 cells by inducing apoptosis and G2/M arrest. Western blot analysis of various signaling markers revealed the ability of DIM to target pathways mediated by Akt,mitogen-activated protein (MAP) kinases, nuclear factor (NF)-κB, and p53, of which the concerted action underlined its antitumor efficacy. The concomitant inactivation of Akt and MAP kinases in response to DIM facilitated the dephosphorylation of the proapoptotic protein Bad at Ser-136 and Ser-112,respectively. Through endoplasmic reticulum (ER) stress, DIM stimulated the activation of p53 via Ser-15 phosphorylation, leading to increased expression of the BH3-only proapoptotic Bcl-2 members Puma and Noxa. Together, these changes decreased the mitochondrial threshold for apoptosis. G2/M arrest might be attributable to the suppressive effect of DIM on the expression of cyclin B1 and cdc25c. As many downstream effectors of the Akt-NF-κB pathway, including glycogen synthase kinase 3β, IκB kinase α, and cyclooxygenase-2, have been shown to promote oral tumorigenesis, the ability of DIMto inhibit this signaling axis underscores its chemopreventive potential in oral cancer. <p><a href="https://greenmedinfo.com/article/33-diindolylmethane-induces-g2m-arrest-and-apoptosis-oral-squamous-cell-carcin" target="_blank">read more</a></p> https://greenmedinfo.com/article/33-diindolylmethane-induces-g2m-arrest-and-apoptosis-oral-squamous-cell-carcin#comments Oral Cancer Squamous cell carcinoma Antiproliferative Apoptotic Cell cycle arrest Chemopreventive In Vitro Study Sat, 16 Mar 2019 01:43:39 +0000 greenmedinfo 181453 at https://greenmedinfo.com 3,3'-Diindolylmethane suppresses growth of human esophageal squamous cancer cells by G1 cell cycle arrest. https://greenmedinfo.com/article/33-diindolylmethane-suppresses-growth-human-esophageal-squamous-cancer-cells-g PMID:  Oncol Rep. 2012 May ;27(5):1669-73. Epub 2012 Jan 27. PMID: 22293900 Abstract Title:  3,3&#039;-Diindolylmethane suppresses growth of human esophageal squamous cancer cells by G1 cell cycle arrest. Abstract:  3,3&#039;-Diindolylmethane (DIM), an active metabolite of indole-3-carbinol, is thought to have antitumor effects in experimental animals and induce apoptosis in various cancer cells. However, the biological functions of DIM in human esophageal cancer cells are unknown. Thus, the purpose of this study was to investigate the cytotoxic effects of DIM in human esophageal squamous cell carcinoma (ESCC) cells to elucidate the molecular mechanism of cell death. Three human ESCC cell lines (TT, TE-8 and TE-12) were used to test the response to DIM. MTT, cell cycle and western blot analyses were conducted. DIM significantly inhibited the proliferation of ESCC cells in a dose- and time-dependent manner. The percentage of G1 phase cells increased 48 h after being treated with DIM. DIM also reduced cyclin D1, cyclin E2, cyclin-dependent kinase (CDK) 4 and CDK 6 activities, and increased p15 and p27 levels. Additionally, DIM diminished pro-caspase-9 protein expression levels and induced increased cleaved poly (ADP-ribose) polymerase levels. These results indicate that DIM leads to G1 phase cell cycle arrest and induces apoptosis by activating caspase-9 in ESCC cells. <p><a href="https://greenmedinfo.com/article/33-diindolylmethane-suppresses-growth-human-esophageal-squamous-cancer-cells-g" target="_blank">read more</a></p> https://greenmedinfo.com/article/33-diindolylmethane-suppresses-growth-human-esophageal-squamous-cancer-cells-g#comments Esophageal Cancer Squamous cell carcinoma Anticarcinogenic Agents Antiproliferative Apoptotic Cell cycle arrest In Vitro Study Sat, 16 Mar 2019 01:08:14 +0000 greenmedinfo 181452 at https://greenmedinfo.com A case report of spontaneous regression of a primary squamous cell lung cancer following biopsy. https://greenmedinfo.com/article/case-report-spontaneous-regression-primary-squamous-cell-lung-cancer-following PMID:  J Med Case Rep. 2018 Mar 12 ;12(1):65. Epub 2018 Mar 12. PMID: 29526162 Abstract Title:  Spontaneous regression of a primary squamous cell lung cancer following biopsy: a case report. Abstract:  BACKGROUND: Spontaneous regression has been defined as occurring when the malignant tumor mass partially or completely disappears without any treatment or as a result of a therapy considered inadequate to influence systemic neoplastic disease. Recently, studies have implicated immunological responses as likely being involved. We report a case of a patient with squamous cell carcinoma of the lung who experienced spontaneous regression following biopsy without other intervention.CASE PRESENTATION: A 57-year-old white man was referred to our pulmonary clinic after an incidental finding of a nodule in the lower lobe of his left lung. Thoracic computed tomography revealed a 2.0× 1.4 × 1.5 cm spiculated nodule in the superior segment of the left lower lobe. Workup identified the mass as a squamous cell carcinoma that was clinically staged as T1M0N0. The patient deferred treatment of this lesion. He undertook no significant lifestyle or medical changes. Three months later, computed tomography revealed that, compared with the initial study, the solitary mass had decreased in size to 1.6 × 0.9 × 0.9 cm. Follow-up computed tomography 1 year after the original workup demonstrated that the nodule had stabilized to its smaller size.CONCLUSIONS: Studies have shown that immunological response can be initiated by trauma to an area. Because the tumor regression became evident in our patient only after the tissue biopsy, his immune response to the surgical procedure seems to be a plausible contributor to the spontaneous regression. Further understanding of spontaneous regression can potentially impact the identification of neoplastic drug targets or even the course of a patient&#039;s treatment plan and goals. <p><a href="https://greenmedinfo.com/article/case-report-spontaneous-regression-primary-squamous-cell-lung-cancer-following" target="_blank">read more</a></p> https://greenmedinfo.com/article/case-report-spontaneous-regression-primary-squamous-cell-lung-cancer-following#comments Lung Cancer Squamous cell carcinoma Spontaneous Regression Human: Case Report Wed, 09 Jan 2019 22:32:23 +0000 greenmedinfo 177204 at https://greenmedinfo.com A case report of spontaneous regression of lung cancer in an elderly patient. https://greenmedinfo.com/article/case-report-spontaneous-regression-lung-cancer-elderly-patient PMID:  Nihon Ronen Igakkai Zasshi. 2017 ;54(4):555-559. PMID: 29212998 Abstract Title:  [Spontaneous regression of lung cancer in an elderly patient: a case report]. Abstract:  An 82 year-old male was referred to us because of a nodule in the upper lobe of his right lung, which was incidentally found by computed tomography (CT) carried out in the course of treating pneumonia. The nodule was identified as non-keratinizing squamous cell carcinoma of the lung by bronchoscopy. A close investigation revealed the tumor to be cT1bN3M1b, clinical Stage IV. Although we only adopted a wait-and-see approach because of his age and his suspected myelodysplastic syndrome, the nodule had regressed on CT images after a year. Fluorodeoxyglucose-positron emission tomography showed apparently decreased uptakes in the lymph nodes and adrenal gland. We considered this to be a systemic observation of spontaneous regression of carcinoma. <p><a href="https://greenmedinfo.com/article/case-report-spontaneous-regression-lung-cancer-elderly-patient" target="_blank">read more</a></p> https://greenmedinfo.com/article/case-report-spontaneous-regression-lung-cancer-elderly-patient#comments Lung Cancer Squamous cell carcinoma Spontaneous Regression Human: Case Report Wed, 09 Jan 2019 23:43:34 +0000 greenmedinfo 177222 at https://greenmedinfo.com A Euphorbia peplus extract demonstrated it was beneficial in patients with non-melanoma skin cancers. https://greenmedinfo.com/article/euphorbia-peplus-extract-demonstrated-it-was-beneficial-patients-non-melanoma- PMID:  Br J Dermatol. 2011 Mar ;164(3):633-6. Epub 2011 Jan 27. PMID: 21375515 Abstract Title:  The sap from Euphorbia peplus is effective against human nonmelanoma skin cancers. Abstract:  BACKGROUND: The sap from Euphorbia peplus, commonly known as petty spurge in the U.K. or radium weed in Australia, has been used as a traditional treatment for a number of cancers.OBJECTIVE: To determine the effectiveness of E. peplus sap in a phase I/II clinical study for the topical treatment of basal cell carcinomas (BCC), squamous cell carcinomas (SCC) and intraepidermal carcinomas (IEC).METHODS: Thirty-six patients, who had refused, failed or were unsuitable for conventional treatment, were enrolled in a phase I/II clinical study. A total of 48 skin cancer lesions were treated topically with 100-300μL of E. peplus sap once daily for 3 days.RESULTS: The complete clinical response rates at 1 month were 82% (n = 28) for BCC, 94% (n = 16) for IEC and 75% (n = 4) for SCC. After a mean follow-up of 15 months these rates were 57%, 75% and 50%, respectively. For superficial lesions2 cm in diameter), are comparable with existing nonsurgical treatments. An active ingredient of E. peplus sap has been identified as ingenol mebutate (PEP005). This clinical study affirms community experience with E. peplus sap, and supports further clinical development of PEP005 for the treatment of BCC, SCC and IEC. https://greenmedinfo.com/article/euphorbia-peplus-extract-demonstrated-it-was-beneficial-patients-non-melanoma-#comments Basal Cell Carcinoma Bowen's Disease Euphorbia peplus Skin Cancer: Non-Melanoma Squamous cell carcinoma Plant Extracts Significant Treatment Outcome Human Study Fri, 13 Nov 2015 20:31:17 +0000 greenmedinfo 121610 at https://greenmedinfo.com A preparation of solasodine glycoalkaloids exhibits significant anti-skin cancer properties. https://greenmedinfo.com/article/preparation-solasodine-glycoalkaloids-exhibits-significant-anti-skin-cancer-pr PMID:  Cancer Lett. 1991 Sep ;59(3):183-92. PMID: 1913614 Abstract Title:  Topical treatment of malignant and premalignant skin lesions by very low concentrations of a standard mixture (BEC) of solasodine glycosides. Abstract:  A cream formulation containing high concentrations (10%) of a standard mixture of solasodine glycosides (BEC) has been shown to be effective in the treatment of malignant and benign human skin tumours. We now report that a preparation (Curaderm) which contains very low concentrations of BEC (0.005%) is effective in the treatment of keratoses, basal cell carcinomas (BCCs) and squamous cell carcinomas (SCCs) of the skin of humans. In an open study, clinical and histological observations indicated that all lesions (56 keratoses, 39 BCCs and 29 SCCs) treated with Curaderm had regressed. A placebo formulation had no effect on a smaller number of treated lesions. Curaderm had no adverse effect on the liver, kidneys or haematopoietic system. https://greenmedinfo.com/article/preparation-solasodine-glycoalkaloids-exhibits-significant-anti-skin-cancer-pr#comments Basal Cell Carcinoma Keratoses Skin Cancer Solasodine glycoalkaloids Squamous cell carcinoma Antineoplastic Agents Animal Study Fri, 07 Oct 2011 14:41:15 +0000 greenmedinfo 68870 at https://greenmedinfo.com A report of 2 cases of spontaneous tumor regression. https://greenmedinfo.com/article/report-2-cases-spontaneous-tumor-regression PMID:  Med Clin (Barc). 1990 Sep 15 ;95(8):306-8. PMID: 2283912 Abstract Title:  [Spontaneous tumor regression. Report of 2 cases]. Abstract:  Two cases of spontaneous tumor regression (STR) occurring in a patient with non Hodgkin lymphoma and in another patient with squamous carcinoma of the lung are presented. Both cases fulfill the criteria of STR defined by Everson and Cole. Recent results obtained in basic and clinical studies have indicated that immunological mechanisms could play an important role in STR. The mediator effects more frequently referred are: 1) generation of antineoplastic cytotoxic cells; 2) production of immunoregulatory cytokines by lymphocytes and monocytes, and 3) possible cross reaction between tumor and bacterial antigens. These mechanisms of action are discussed in relation to the presented cases. <p><a href="https://greenmedinfo.com/article/report-2-cases-spontaneous-tumor-regression" target="_blank">read more</a></p> https://greenmedinfo.com/article/report-2-cases-spontaneous-tumor-regression#comments Non-Hodgkin Lymphoma Squamous cell carcinoma Spontaneous Tumor Regression Human: Case Report Sat, 23 Jan 2021 03:20:19 +0000 greenmedinfo 233366 at https://greenmedinfo.com Administration of berberine and resveratrol could be used for cancer treatment. https://greenmedinfo.com/article/administration-berberine-and-resveratrol-could-be-used-cancer-treatment PMID:  Anticancer Agents Med Chem. 2019 Apr 5. Epub 2019 Apr 5. PMID: 30950357 Abstract Title:  Effects of Resveratrol, Berberine, and Their Combinations on Reactive Oxygen Species, Survival, and Apoptosis in Human Squamous Carcinoma (SCC-25) Cells. Abstract:  BACKGROUND: Levels of cellular Reactive Oxygen Species (ROS) influence the oxidized/reduced states of cellular proteins, and create redox-signaling pathways that can activate transcription factors, kinases, and phosphatases. ROS levels can be increased radically by external factors, including ionizing and UV radiation or exposure to chemical compounds. These increased ROS levels can in turn lead to oxidative damage of DNA. Natural plant treatments against cancer can modulate that processes by inducing or decreasing ROS production.METHODS: Here we report new observations that squamous carcinoma (SCC-25) cells, exposed to 24 hours of combined resveratrol and berberine treatment contained increased ROS levels. Using flow cytometry, for drug activity characteristic, an accumulation of ROS was observed. Combination of different dyes, CellROX Green (Life Technologies) and DCFH-DA (Sigma), allowed for flow cytometric estimation of levels of cellular ROS as well as cellular localization.RESULTS: Live staining and microscopic observations confirmed the accumulation of ROS in SCC-25 cells following combination treatment at concentrations of 10µg/ml. Additionally, the cytotoxicity of the compounds were significantly improved after their, combined application. Additive effects were observed for doses lower than the calculated IC50 of berberine [IC50=23 µg/ml] and resveratrol [IC50=9 µg/ml]. Viability (MTS) assays and analysis of isobolograms revealed a significant impact on cell viability upon combination treatment.CONCLUSION: These results suggest that administration of berberine, in the presence of resveratrol, could be decreased even to 50% (half the IC50 for berberine) for cancer treatment. <p><a href="https://greenmedinfo.com/article/administration-berberine-and-resveratrol-could-be-used-cancer-treatment" target="_blank">read more</a></p> https://greenmedinfo.com/article/administration-berberine-and-resveratrol-could-be-used-cancer-treatment#comments Berberine Resveratrol Squamous cell carcinoma Antiproliferative Chemopreventive Natural Substance Synergy In Vitro Study Sun, 14 Apr 2019 19:13:25 +0000 greenmedinfo 185027 at https://greenmedinfo.com Allicin was shown to have good efficacy in repressing cell proliferation as well as facilitating cell apoptosis in oral tongue squamous cell carcinoma. https://greenmedinfo.com/article/allicin-was-shown-have-good-efficacy-repressing-cell-proliferation-well-facili PMID:  Onco Targets Ther. 2020 ;13:13183-13189. Epub 2020 Dec 29. PMID: 33402832 Abstract Title:  The effect of allicin on cell proliferation and apoptosis compared to blank control and cis-platinum in oral tongue squamous cell carcinoma. Abstract:  Background: Oral tongue squamous cell carcinoma (OTSCC) has aggressive clinical behavior with poor prognosis. Allicin plays a tumor-suppressive role in various cancers, although the role of allicin in OTSCC is unknown. We aimed to investigate the effect of allicin on cell proliferation and apoptosis compared to blank control and cis-platinum in OTSCC.Methods: Tca-8113 and SCC-25 cells were treated with non-stimulated control, 12.5µg/mL allicin, 25 µg/mL allicin, 50 µg/mL allicin, and 40 µg/mL cis-platinum, which were divided into blank control, allicin 12.5 µg/mL, allicin 25 µg/mL, allicin 50 µg/mL, and cis-platinum 40 µg/mL groups, respectively. Cell proliferation was determined by the Cell Counting Kit-8 assay. Cell apoptosis was detected by annexin V/propidium iodide and Western blot assays.Results: In Tca-8113 and SCC-25 cells, cell proliferation was inhibited by 40μg/mL cis-platinum, 12.5 μg/mL allicin, 25 μg/mL allicin, and 50 μg/mL allicin. Cell apoptosis was promoted by 40 μg/mL cis-platinum, 12.5 μg/mL allicin, 25 μg/mL allicin, and 50 μg/mL allicin, while compared to 40 μg/mL cis-platinum, it was increased by 50 μg/mL allicin. Western blot assay revealed that expression of pro-apoptosis protein Bax and C-Caspase 3 increased, but apoptosis-inhibitory protein Bcl-2 expression decreased with 40 μg/mL cis-platinum, 12.5 μg/mL allicin, 25 μg/mL allicin, and 50 μg/mL allicin, while compared to 40 μg/mL cis-platinum, Bax and C-Caspase 3 expression was increased by 50 μg/mL allicin.Conclusion: Allicin was shown to have good efficacy in repressing cell proliferation as well as facilitating cell apoptosis in OTSCC. <p><a href="https://greenmedinfo.com/article/allicin-was-shown-have-good-efficacy-repressing-cell-proliferation-well-facili" target="_blank">read more</a></p> https://greenmedinfo.com/article/allicin-was-shown-have-good-efficacy-repressing-cell-proliferation-well-facili#comments Allicin Oral Cancer Squamous cell carcinoma Antiproliferative In Vitro Study Sun, 14 Mar 2021 21:17:38 +0000 greenmedinfo 236360 at https://greenmedinfo.com