Pseudomonas aeruginosa https://greenmedinfo.com/taxonomy/term/5636/all en Anti-quorum sensing and biofilm inhibitory activity of Apium graveolens L. oleoresin. https://greenmedinfo.com/article/anti-quorum-sensing-and-biofilm-inhibitory-activity-apium-graveolens-l-oleores PMID:  J Food Sci Technol. 2020 Jul ;57(7):2414-2422. Epub 2020 Jan 28. PMID: 32549591 Abstract Title:  Anti-quorum sensing and biofilm inhibitory activity ofL. oleoresin. Abstract:  L. (Apiaceae) is a dietary herb used as a spice, condiment and medicine.(Celery) has been studied for its antimicrobial property and for its application as flavours in food industry. The present study investigated theoleoresin as an anti-quorum sensing and antibiofilm agent. The quorum sensing and biofilm inhibition study was carried out using biosensor strainsCV12472 andPAO1. The MIC of celery oleoresin againstCV12472 andPAO1 was 10 and 25% v/v, respectively. Inhibition of violacein and biofilm formation was tested at concentrations of oleoresins ranging from 1.56 and 50% v/v. The oleoresins showed a concentration dependent QS inhibitory activity and at sub-MIC of 6.25 and 12.5% v/v, the oleoresins significantly inhibited violacein production and biofilm formation ( <p><a href="https://greenmedinfo.com/article/anti-quorum-sensing-and-biofilm-inhibitory-activity-apium-graveolens-l-oleores" target="_blank">read more</a></p> https://greenmedinfo.com/article/anti-quorum-sensing-and-biofilm-inhibitory-activity-apium-graveolens-l-oleores#comments Celery Pseudomonas aeruginosa Quorum Sensing Inhibition Bacterial Infections: Resistance/Biofilm Formation In Vitro Study Tue, 11 Aug 2020 23:25:56 +0000 greenmedinfo 225094 at https://greenmedinfo.com 6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition https://greenmedinfo.com/article/6-gingerol-reduces-pseudomonas-aeruginosa-biofilm-formation-and-virulence-quor PMID:  Sci Rep. 2015 ;5:8656. Epub 2015 Mar 2. PMID: 25728862 Abstract Title:  6-Gingerol reduces Pseudomonas aeruginosa biofilm formation and virulence via quorum sensing inhibition. Abstract:  Pseudomonas aeruginosa is a well-known pathogenic bacterium that forms biofilms and produces virulence factors via quorum sensing (QS). Interfering with normal QS interactions between signal molecules and their cognate receptors is a developing strategy for attenuating its virulence. Here we tested the hypothesis that 6-gingerol, a pungent oil of fresh ginger, reduces biofilm formation and virulence by antagonistically binding to P. aeruginosa QS receptors. In silico studies demonstrated molecular binding occurs between 6-gingerol and the QS receptor LasR through hydrogen bonding and hydrophobic interactions. Experimentally 6-gingerol reduced biofilm formation, several virulence factors (e.g., exoprotease, rhamnolipid, and pyocyanin), and mice mortality. Further transcriptome analyses demonstrated that 6-gingerol successfully repressed QS-induced genes, specifically those related to the production of virulence factors. These results strongly support our hypothesis and offer insight into the molecular mechanism that caused QS gene repression. https://greenmedinfo.com/article/6-gingerol-reduces-pseudomonas-aeruginosa-biofilm-formation-and-virulence-quor#comments Gingerol Pseudomonas aeruginosa Anti-Bacterial Agents In Vitro Study Fri, 27 Nov 2015 21:45:44 +0000 greenmedinfo 121841 at https://greenmedinfo.com A combination of pomegranate rind extract, copper and vitamin C have antimicrobial activity against multidrug resistant Pseudomonas aeruginosa. https://greenmedinfo.com/article/combination-pomegranate-rind-extract-copper-and-vitamin-c-have-antimicrobial-a PMID:  Br J Biomed Sci. 2009;66(3):129-32. PMID: 19839222 Abstract Title:  Antimicrobial pomegranate rind extracts: enhancement by Cu(II) and vitamin C combinations against clinical isolates of Pseudomonas aeruginosa. Abstract:  Recently, natural products have been further evaluated as sources of antimicrobial agents with efficacies against a variety of microorganisms. This study reports the antimicrobial activities of pomegranate rind extract (PRE) in combination with Fe(II) and Cu(II) salts against extended-spectrum multidrug-resistant Pseudomonas aeruginosa. Antimicrobial suspension assays were carried out using aqueous extract of pomegranate alone or in combination with metals salts against P. aeruginosa. The extract:metal salt combination was also enhanced with the addition of vitamin C. Marked activities were observed for the aqueous PRE/Cu(II) preparations, which were greatly enhanced by the addition of the reductant vitamin C. In contrast, the aqueous PRE/Fe(II) preparations were inactive, regardless of addition of vitamin C. The combination of PRE and Cu(II) salts and vitamin C showed the greatest activity against clinical isolates of P. aeruginosa. These results warrant further investigation of PRE as a potential source of new antimicrobial agents. https://greenmedinfo.com/article/combination-pomegranate-rind-extract-copper-and-vitamin-c-have-antimicrobial-a#comments Copper Infection: Antibiotic Resistant Pomegranate Pseudomonas aeruginosa Pseudomonas Infections Vitamin C Anti-Bacterial Agents Multi-Drug Resistant Pathogens In Vitro Study Sun, 27 Dec 2009 00:07:59 +0000 greenmedinfo 48387 at https://greenmedinfo.com A cranberry extract rich in proanthocyanidins can restrict the virulence of P. aeruginosa. https://greenmedinfo.com/article/cranberry-extract-rich-proanthocyanidins-can-restrict-virulence-p-aeruginosa PMID:  Sci Rep. 2016 ;6:30169. Epub 2016 Aug 9. PMID: 27503003 Abstract Title:  Cranberry-derived proanthocyanidins impair virulence and inhibit quorum sensing of Pseudomonas aeruginosa. Abstract:  Bacteria have evolved multiple strategies for causing infections that include producing virulence factors, undertaking motility, developing biofilms, and invading host cells. N-acylhomoserine lactone (AHL)-mediated quorum sensing (QS) tightly regulates the expression of multiple virulence factors in the opportunistic pathogenic bacterium Pseudomonas aeruginosa. Thus, inhibiting QS could lead to health benefits. In this study, we demonstrate an anti-virulence activity of a cranberry extract rich in proanthocyanidins (cerPAC) against P. aeruginosa in the model host Drosophila melanogaster and show this is mediated by QS interference. cerPAC reduced the production of QS-regulated virulence determinants and protected D. melanogaster from fatal infection by P. aeruginosa PA14. Quantification of AHL production using liquid chromatography-mass spectrometry confirmed that cerPAC effectively reduced the level of AHLs produced by the bacteria. Furthermore, monitoring QS signaling gene expression revealed that AHL synthases LasI/RhlI and QS transcriptional regulators LasR/RhlR genes were inhibited and antagonized, respectively, by cerPAC. Molecular docking studies suggest that cranberry-derived proanthocyanidin binds to QS transcriptional regulators, mainly interacting with their ligand binding sites. These findings provide insights into the underlying mechanisms of action of a cerPAC to restrict the virulence of P. aeruginosa and can have implications in the development of alternative approaches to control infections. https://greenmedinfo.com/article/cranberry-extract-rich-proanthocyanidins-can-restrict-virulence-p-aeruginosa#comments Cranberry Cranberry Proanthocyanidins Pseudomonas aeruginosa Anti-Bacterial Agents Plant Extracts In Vitro Study Tue, 30 Aug 2016 23:06:49 +0000 greenmedinfo 134416 at https://greenmedinfo.com A cultured broth of Ganoderma lucidum exhibited antioxidant, antibacterial and α-amylase inhibitory activities. https://greenmedinfo.com/article/cultured-broth-ganoderma-lucidum-exhibited-antioxidant-antibacterial-and-amyla PMID:  J Food Sci Technol. 2017 Oct ;54(11):3724-3730. Epub 2017 Sep 7. PMID: 29051668 Abstract Title:  Culture broth of Ganoderma lucidum exhibited antioxidant, antibacterial andα-amylase inhibitory activities. Abstract:  Culture broth of Ganoderma lucidum was determined for antioxidant, antibacterial andα-amylase inhibitory activities. The culture broth contained protein as determined by Bradford method equaled to 0.2 mg/ml and total phenol content as 0.078 mg GAE/mg protein (0.557 mg GAE/g extract). It exhibited radicals scavenging activities against ABTS(+·) and DPPH(·) radicals with a half maximal inhibitory concentration (IC50) less than 1.70 ± 0.02 and 2.28 ± 0.02 µg protein/ml, respectively and reducing power equaled to 4.38 ± 0.02 µmol Trolox/µg protein as investigated by ferric ion reducing antioxidant power method. The culture broth experimented into two approached; (1) treated with pronase and (2) filtered through a membrane with 10 kDa molecular weight cut-off (MWCO). The pronase-treated culture broth exhibited insignificant lower antioxidant activities, but the retained culture broth 10 kDa MWCO resulted in significant decrease in antioxidant activities suggesting that the small proteins might play the key role in antioxidant activity. The culture broth could protect DNA damage from hydroxyl radicals ((·)OH) generated by Fenton&#039;s reaction. This culture broth showed antibacterial activity towards pathogenic strains Staphylococcus epidermidis and Pseudomonas aeruginosa and also had an interesting α-amylase inhibitory activity. This study suggested that apart from the fruiting bodies and the mycelial of G. lucidum, its culture broth also had potential applications as a value-added ingredient in the product such as in cosmetics and in nutraceuticals. <p><a href="https://greenmedinfo.com/article/cultured-broth-ganoderma-lucidum-exhibited-antioxidant-antibacterial-and-amyla" target="_blank">read more</a></p> https://greenmedinfo.com/article/cultured-broth-ganoderma-lucidum-exhibited-antioxidant-antibacterial-and-amyla#comments Pseudomonas aeruginosa Reishi Mushroom Staphylococcus epidermidis Alpha-amylase inhibitor Anti-Bacterial Agents Antioxidants In Vitro Study Fri, 17 Nov 2017 03:03:07 +0000 greenmedinfo 155940 at https://greenmedinfo.com A maple syrup extract exhibited antimicrobial activity as well as strong synergistic interaction with selected antibiotics against gram negative clinical strains of bacteria. https://greenmedinfo.com/article/maple-syrup-extract-exhibited-antimicrobial-activity-well-strong-synergistic-i PMID:  Appl Environ Microbiol. 2015 Mar 27. Epub 2015 Mar 27. PMID: 25819960 Abstract Title:  Polyphenolic Extract from Maple Syrup Potentiates Antibiotic Susceptibility and Reduces Biofilm Formation of Pathogenic Bacteria. Abstract:  Phenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a Phenolic-Rich Maple Syrup Extract (PRMSE). PRMSE exhibited antimicrobial activity as well as strong synergistic interaction with selected antibiotics against Gram-negative clinical strains of Escherichia coli, Proteus mirabilis and Pseudomonas aeruginosa. Amongst the phenolic constituents of PRMSE, catechol exhibited strong synergy with antibiotics as well as with other phenolic components of PRMSE against bacterial growth. At sub-lethal concentrations, PRMSE and catechol efficiently reduced biofilm formation and increased the susceptibility of bacterial biofilms to antibiotics. In an effort to elucidate the mechanism for the observed synergy with antibiotics, PRMSE was found to increase outer-membrane permeability of all bacterial strains and effectively inhibit efflux pump activity. Furthermore, transcriptome analysis revealed that PRMSE significantly repressed multiple drug resistance genes as well as genes associated with motility, adhesion, biofilm formation and virulence. Overall, this study provides a proof-of-concept and starting point for investigating the molecular mechanism of the reported increase in bacterial antibiotic susceptibility in the presence of PRMSE. https://greenmedinfo.com/article/maple-syrup-extract-exhibited-antimicrobial-activity-well-strong-synergistic-i#comments Bacterial Infections: Resistance/Biofilm Formation Escherichia coli Infections Maple Syrup Pseudomonas aeruginosa Anti-Bacterial Agents Antimicrobial Natural Substance/Drug Synergy In Vitro Study Tue, 21 Apr 2015 18:37:58 +0000 greenmedinfo 116967 at https://greenmedinfo.com A methanolic extract of Musa paradisiaca showed wound healing activity in Wistar albino rats. https://greenmedinfo.com/article/methanolic-extract-musa-paradisiaca-showed-wound-healing-activity-wistar-albin PMID:  Int Wound J. 2014 Sep 16. Epub 2014 Sep 16. PMID: 25224162 Abstract Title:  Wound healing activity of methanolic stem extract of Musa paradisiaca Linn. (Banana) in Wistar albino rats. Abstract:  This study is designed to explore the phytochemical, antibacterial and wound healing activity of methanolic stem extract of Musa paradisiaca Linn. (Banana). The phytochemical analysis was performed for the methanolic stem extract of Musa paradisiaca Linn. Results indicates that the Musa paradisiaca Linn. was rich in glucosides, tannins and alkaloids, saponins, flavonoids and phenols were present in moderate quantities. The extract shows antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus with the zone of inhibition of Pseudomonas aeruginosa was 21 mm and Staphylococcus aureus was 19 mm at concentration of 500 µg/disc. The minimum inhibitory concentration (MIC) was also evaluated for the extract. Wistar albino rats were selected for wound healing activity. The burn wound was created by using red hot steel rod from above the hind limb region. The methanolic extract was applied on the wound and the progressive changes were monitored every day. The wound contraction rate was absorbed based on the histopathological examination. It was concluded that the methanolic extract of Musa paradisiaca Linn. showed greater healing activity compared to control in Wistar albino rats. https://greenmedinfo.com/article/methanolic-extract-musa-paradisiaca-showed-wound-healing-activity-wistar-albin#comments Banana Pseudomonas aeruginosa Staphylococcus aureus infection Wound Healing Anti-Bacterial Agents Plant Extracts Animal Study Mon, 25 Apr 2016 21:04:11 +0000 greenmedinfo 126475 at https://greenmedinfo.com A review of green tea catechins and their uses in treating infectious diseases. https://greenmedinfo.com/article/review-green-tea-catechins-and-their-uses-treating-infectious-diseases PMID:  Biomed Res Int. 2018 ;2018:9105261. Epub 2018 Jul 17. PMID: 30105263 Abstract Title:  Green Tea Catechins: Their Use in Treating and Preventing Infectious Diseases. Abstract:  Green tea is one of the most popular drinks consumed worldwide. Produced mainly in Asian countries from the leaves of theplant, the potential health benefits have been widely studied. Recently, researchers have studied the ability of green tea to eradicate infectious agents and the ability to actually prevent infections. The important components in green tea that show antimicrobial properties are the catechins. The four main catechins that occur in green tea are (-)-epicatechin (EC), (-)-epicatechin-3-gallate (ECG), (-)-epigallocatechin (EGC), and (-)-epigallocatechin-3-gallate (EGCG). Of these catechins, EGCG and EGC are found in the highest amounts in green tea and have been the subject of most of the studies. These catechins have been shown to demonstrate a variety of antimicrobial properties, both to organisms affected and in mechanisms used. Consumption of green tea has been shown to distribute these compounds and/or their metabolites throughout the body, which allows for not only the possibility of treatment of infections but also the prevention of infections. <p><a href="https://greenmedinfo.com/article/review-green-tea-catechins-and-their-uses-treating-infectious-diseases" target="_blank">read more</a></p> https://greenmedinfo.com/article/review-green-tea-catechins-and-their-uses-treating-infectious-diseases#comments Catechin EGCG (Epigallocatechin gallate) Epicatechin Escherichia coli Infections Helicobacter Pylori Infection Pseudomonas aeruginosa Staphylococcus aureus infection Steptococcus Mutans Infections Antimicrobial Quorum Sensing Inhibition Natural Substance/Drug Synergy Review Fri, 07 Sep 2018 03:17:05 +0000 greenmedinfo 170330 at https://greenmedinfo.com A review of the health promoting vaules and food applications of black cumin essential oil. https://greenmedinfo.com/article/review-health-promoting-vaules-and-food-applications-black-cumin-essential-oil PMID:  J Food Sci Technol. 2015 Oct ;52(10):6136-42. Epub 2015 Mar 27. PMID: 26396361 Abstract Title:  Health-promoting value and food applications of black cumin essential oil: an overview. Abstract:  Black cumin (Nigella sativa L.) seeds and its essential oil have been widely used in functional foods, nutraceuticals and pharmaceutical products. Analysis of Nigella sativa essential oil using GC and GC-MS resulted in the identification of many bioactive compounds representing ca. 85 % of the total content. The main compounds included p-cymene, thymoquinone, α-thujene, longifolene, β-pinene, α-pinene and carvacrol. Nigella sativa essential oil exhibited different biological activities including antifungal, antibacterial and antioxidant potentials. Nigella sativa essential oil showed complete inhibition zones against different Gram-negative and Gram-positive bacteria including Penicillium citrinum Bacillus cereus, Bacillus subtilis, Staphylococcus aureus and Pseudomonas aeruginosa. The essential oil showed stronger antioxidant potential in comparison with synthetic antioxidants (i.e., BHA and BHT) in a rapeseed oil model system. The oil exhibited also stronger radical scavenging activity against DPPH·radical in comparison with synthetic antioxidants. The diversity of applications to which Nigella sativa essential oil can be put gives this oil industrial importance. https://greenmedinfo.com/article/review-health-promoting-vaules-and-food-applications-black-cumin-essential-oil#comments Bacillus Cereus infection Bacillus subtilis infections Gram-Negative Bacterial Infections Gram-Positive Bacterial Infections Nigella sativa (aka Black Seed) Pseudomonas aeruginosa Staphylococcus aureus infection Anti-Bacterial Agents Antifungal Agents Antioxidants Essential Oils Review Thu, 22 Oct 2015 02:34:44 +0000 greenmedinfo 121257 at https://greenmedinfo.com A systematic review on antimicrobial and antiparasitic activity of Eurycoma longifolia Jack. https://greenmedinfo.com/article/systematic-review-antimicrobial-and-antiparasitic-activity-eurycoma-longifolia PMID:  Biomed Res Int. 2022 ;2022:4999797. Epub 2022 Jul 6. PMID: 35845951 Abstract Title:  A Systematic Review on Antimicrobial and Antiparasitic Activity ofJack (Tongkat Ali). Abstract:  or Tongkat Ali (family: Simaroubaceae) has the potential to be utilised as an antimicrobial and antiparasitic agent that correlated with its traditional use to treat jaundice, malaria, antiseptic agent, and many more. This review is aimed at systematically sieving through articles regarding the antimicrobial and antiparasitic activity of. A total of 123 studies have been found using suitable keywords and manually searched from previous studies through the four databases. After title screening and abstract examination, 56 articles were excluded due to duplication and not meeting the acceptance criteria. 67 articles were assessed on full-text accessibility, 31 studies remained, and this number decreased to 20 articles after a careful examination of the full-text articles. Among the 20 articles selected, 17 articles proved the potential ofas an antimicrobial and antiparasitic agent efficiently. 2 selected articles showed partial positive results, which specified specific microorganisms tested. In contrast, another 1 article gave a completely negative result. As for the conclusion, current studies highlighted by this review may shed light on the future direction of studies concerningas a novel antimicrobial and antiparasitic agent. However, more research should be done in the future focusing on the efficiency offor veterinary medicine utilisation. <p><a href="https://greenmedinfo.com/article/systematic-review-antimicrobial-and-antiparasitic-activity-eurycoma-longifolia" target="_blank">read more</a></p> https://greenmedinfo.com/article/systematic-review-antimicrobial-and-antiparasitic-activity-eurycoma-longifolia#comments Bacillus Cereus infection Bacillus subtilis infections Candida Albicans Escherichia coli Infections Plasmodium Infections Pseudomonas aeruginosa Schistosomiasis Shigellosis Staphylococcus aureus infection Steptococcus Mutans Infections Tongkat Ali Anti-Bacterial Agents Antimicrobial Antiparasitic Agents Plant Extracts Review Wed, 08 Feb 2023 03:49:02 +0000 greenmedinfo 270754 at https://greenmedinfo.com A. indica bark extract has a potent antibacterial property against S. aureus, E. faecalis, P. aeruginosa, and P. mirabilis. https://greenmedinfo.com/article/indica-bark-extract-has-potent-antibacterial-property-against-s-aureus-e-faeca PMID:  J Pharm Bioallied Sci. 2019 Jan-Mar;11(1):33-37. PMID: 30906137 Abstract Title:  Effect ofCrude Bark Extracts Concentrations against Gram-Positive and Gram-Negative Bacterial Pathogens. Abstract:  Background: Today&#039;s pharmaceutical markets are crowded with antibacterial agents, yet their toxicities and resistance are always contentious.is used in India as an age-old treatment for diverse medical conditions. This study was put forth to analyze the antibacterial action ofbark extract on different bacterial pathogens.Materials and Methods: bark extract was prepared using Soxhlet extraction method. The bark extract ofwas screened for antibacterial activity by agar well diffusion assay technique against,,, and Proteus mirabilis. Ciprofloxacin (5µg per disc) was used as positive control. All assays were carried out under strict aseptic precautions. All the concentrations included were duplicated, and the results obtained were the average of two independent experiments. Broth microdilution method was used to study the minimum inhibitory concentration of the bark extract of.Results: bark extract has shown antibacterial activity against all concentrations of bark extract, whereas antibacterial activity againstwas observed at higher concentration of&gt;500µg/mL. The bark extract ofshowed zone of clearance against,, andat all concentrations. Zone of inhibition was observed in higher concentrations against.Conclusion: This study has shown thatbark extract has a potent antibacterial property against,,, and. Further isolation of bioactive compounds may lead to a new scope in the treatment of bacterial infections. <p><a href="https://greenmedinfo.com/article/indica-bark-extract-has-potent-antibacterial-property-against-s-aureus-e-faeca" target="_blank">read more</a></p> https://greenmedinfo.com/article/indica-bark-extract-has-potent-antibacterial-property-against-s-aureus-e-faeca#comments Enterococcus Infections Neem Pseudomonas aeruginosa Staphylococcus aureus infection Anti-Bacterial Agents In Vitro Study Sat, 30 Mar 2019 10:24:37 +0000 greenmedinfo 183934 at https://greenmedinfo.com A. vera gel could be active against P. aeruginosa in wound infections at various concentrations. https://greenmedinfo.com/article/vera-gel-could-be-active-against-p-aeruginosa-wound-infections-various-concent PMID:  Chemother Res Pract. 2015 ;2015:639806. Epub 2015 Jul 22. PMID: 26266047 Abstract Title:  Aloe vera Gel: Effective Therapeutic Agent against Multidrug-Resistant Pseudomonas aeruginosa Isolates Recovered from Burn Wound Infections. Abstract:  Objective. Aloe vera is an herbal medicinal plant with biological activities, such as antimicrobial, anticancer, anti-inflammatory, and antidiabetic ones, and immunomodulatory properties. The purpose of this study was investigation of in vitro antimicrobial activity of A. vera gel against multidrug-resistant (MDR) Pseudomonas aeruginosa isolated from patients with burn wound infections. Methods. During a 6-month study, 140 clinical isolates of P. aeruginosa were collected from patients admitted to the burn wards of a hospital in Tehran, Iran. Antimicrobial susceptibility test was carried out against the pathogens using the A. vera gel and antibiotics (imipenem, gentamicin, and ciprofloxacin). Results. The antibiogram revealed that 47 (33.6%) of all isolates were MDR P. aeruginosa. The extract isolated from A. vera has antibacterial activity against all of isolates. Also, 42 (89.4%) isolates were inhibited by A. vera gel extract at minimum inhibitory concentration (MIC)≤ 200 µg/mL. MIC value of A. vera gel for other isolates (10.6%) was 800 µg/mL. All of MDR P. aeruginosa strains were inhibited by A. vera at similar MIC50 and MIC90 200 µg/mL. Conclusion. Based on our results, A. vera gel at various concentrations can be used as an effective antibacterial agent in order to prevent wound infection caused by P. aeruginosa. https://greenmedinfo.com/article/vera-gel-could-be-active-against-p-aeruginosa-wound-infections-various-concent#comments Aloe Vera Pseudomonas aeruginosa Antimicrobial Multidrug Resistance Human In Vitro Fri, 14 Aug 2015 01:44:56 +0000 greenmedinfo 119795 at https://greenmedinfo.com Administration of curcumin may prevent suppurative otitis media following tympanostomy tube insertion. https://greenmedinfo.com/article/administration-curcumin-may-prevent-suppurative-otitis-media-following-tympano PMID:  Mikrobiyol Bul. 2015 Jul ;49(3):340-51. PMID: 26313276 Abstract Title:  [Can curcumin prevent the formation of Pseudomonas aeruginosa biofi lm on the surface of tympanostomy tubes?]. Abstract:  Formation of bacterial biofilm on the surface of tympanostomy tubes are held responsible in the pathogenesis of post-tympanostomy tube otorrhea. To prevent the formation of biofilm, various methods were employed and varying degrees of success have been achieved. In some recent studies curcumin, which is the fenolic form of Curcuma longa (turmeric), has been pointed out to have inhibitory effects on virulence factors of Pseudomonas aeruginosa. The aim of this study was to investigate whether the administration of curcumin is able to prevent the formation of P.aeruginosa biofilm on the surface of silicone tympanostomy tubes in vitro conditions. For this purpose, qualitative and quantitative analysis of P.aeruginosa biofilm created on the surface of the tympanostomy tubes were performed following a period of 48 hours incubation in microplate wells that contained decreasing concentrations of curcumin. For qualitative analysis, specimens were evaluated with an environmental scanning electron microscope for the existence of biofilm. For the quantitative analysis, bacteria attached to the tube surface was detached using a combination of vortexing and sonication. Following serial dilutions, the obtained solution was then inoculated on the sheep blood agar plates using calibrated loop, incubated for 24 hours and the colony forming unit (CFU) per mL were recorded. Environmental scanning electron microscope analysis revealed that 100µg/mL of curcumin could prevent formation of the biofilm. Lower concentrations of curcumin could not prevent the biofilm formation. Qualitative analysis also revealed that when the concentrations of curcumin in the wells were decreased, the number of CFU/mL was increased significantly. Mean numberof CFU in 100 µg/mL and 12.5 µg/mL groups were 35 ± 7.07 and 650 ± 494, respectively. Curcumin could prevent formation of P.aeruginosa biofilm on the surface of tympanostomy tubes in vitro with concentrations lower than the MIC value. The results of the present study show that local administration of curcumin may prevent suppurative otitis media following tympanostomy tube insertion, keep the patency of the tube and decrease the rate of treatment failure. In vivo studies are needed to support the in vitro anti-biofilm action of curcumin on tympanostomy tubes. https://greenmedinfo.com/article/administration-curcumin-may-prevent-suppurative-otitis-media-following-tympano#comments Curcumin Otitis media Pseudomonas aeruginosa Anti-Bacterial Agents Grommet Risk Reduction In Vitro Study Tue, 01 Sep 2015 19:52:28 +0000 greenmedinfo 120133 at https://greenmedinfo.com Ajoene could be used to control the expression of virulence factors in Pseudomonas aeruginosa and Staphylococcus aureus. https://greenmedinfo.com/article/ajoene-could-be-used-control-expression-virulence-factors-pseudomonas-aerugino n/a PMID:  Sci Rep. 2017 Aug 29 ;7(1):9857. Epub 2017 Aug 29. PMID: 28851971 Abstract Title:  A broad range quorum sensing inhibitor working through sRNA inhibition. Abstract:  For the last decade, chemical control of bacterial virulence has received considerable attention. Ajoene, a sulfur-rich molecule from garlic has been shown to reduce expression of key quorum sensing regulated virulence factors in the opportunistic pathogen Pseudomonas aeruginosa. Here we show that the repressing effect of ajoene on quorum sensing occurs by inhibition of small regulatory RNAs (sRNA) in P. aeruginosa as well as in Staphylococcus aureus, another important human pathogen that employs quorum sensing to control virulence gene expression. Using various reporter constructs, we found that ajoene lowered expression of the sRNAs RsmY and RsmZ in P. aeruginosa and the small dual-function regulatory RNA, RNAIII in S. aureus, that controls expression of key virulence factors. We confirmed the modulation of RNAIII by RNA sequencing and found that the expression of many QS regulated genes encoding virulence factors such as hemolysins and proteases were lowered in the presence of ajoene in S. aureus. Importantly, our findings show that sRNAs across bacterial species potentially may qualify as targets of anti-virulence therapy and that ajoene could be a lead structure in search of broad-spectrum compounds transcending the Gram negative-positive borderline. https://greenmedinfo.com/article/ajoene-could-be-used-control-expression-virulence-factors-pseudomonas-aerugino#comments Garlic Pseudomonas aeruginosa Staphylococcus aureus infection Anti-Bacterial Agents MicroRNA modulator Quorum Sensing Inhibition Biofilm In Vitro Study Wed, 27 Dec 2017 12:52:34 +0000 greenmedinfo 157747 at https://greenmedinfo.com Ajoene, a sulfur-rich molecule from garlic, inhibits genes controlled by quorum sensing. https://greenmedinfo.com/article/ajoene-sulfur-rich-molecule-garlic-inhibits-genes-controlled-quorum-sensing PMID:  Antimicrob Agents Chemother. 2012 May ;56(5):2314-25. Epub 2012 Feb 6. PMID: 22314537 Abstract Title:  Ajoene, a sulfur-rich molecule from garlic, inhibits genes controlled by quorum sensing. Abstract:  In relation to emerging multiresistant bacteria, development of antimicrobials and new treatment strategies of infections should be expected to become a high-priority research area. Quorum sensing (QS), a communication system used by pathogenic bacteria like Pseudomonas aeruginosa to synchronize the expression of specific genes involved in pathogenicity, is a possible drug target. Previous in vitro and in vivo studies revealed a significant inhibition of P. aeruginosa QS by crude garlic extract. By bioassay-guided fractionation of garlic extracts, we determined the primary QS inhibitor present in garlic to be ajoene, a sulfur-containing compound with potential as an antipathogenic drug. By comprehensive in vitro and in vivo studies, the effect of synthetic ajoene toward P. aeruginosa was elucidated. DNA microarray studies of ajoene-treated P. aeruginosa cultures revealed a concentration-dependent attenuation of a few but central QS-controlled virulence factors, including rhamnolipid. Furthermore, ajoene treatment of in vitro biofilms demonstrated a clear synergistic, antimicrobial effect with tobramycin on biofilm killing and a cease in lytic necrosis of polymorphonuclear leukocytes. Furthermore, in a mouse model of pulmonary infection, a significant clearing of infecting P. aeruginosa was detected in ajoene-treated mice compared to a nontreated control group. This study adds to the list of examples demonstrating the potential of QS-interfering compounds in the treatment of bacterial infections. <p><a href="https://greenmedinfo.com/article/ajoene-sulfur-rich-molecule-garlic-inhibits-genes-controlled-quorum-sensing" target="_blank">read more</a></p> https://greenmedinfo.com/article/ajoene-sulfur-rich-molecule-garlic-inhibits-genes-controlled-quorum-sensing#comments Garlic Pseudomonas aeruginosa Upper Respiratory Infections Anti-Bacterial Agents Quorum Sensing Inhibition Drug Synergy Plant Extracts Bacterial Tue, 24 Apr 2018 22:38:34 +0000 greenmedinfo 163217 at https://greenmedinfo.com