Melanoma: Metastatic https://greenmedinfo.com/taxonomy/term/5743/all en A proteinase inhibitor from potato exhibits inhibitory activity against melanoma cell invasion in vitro. https://greenmedinfo.com/article/proteinase-inhibitor-potato-exhibits-inhibitory-activity-against-melanoma-cell PMID:  Biol Chem. 2002 May;383(5):839-42. PMID: 12108549 Abstract Title:  Effect of cysteine proteinase inhibitors on murine B16 melanoma cell invasion in vitro. Abstract:  Various types of proteinases are implicated in the malignant progression of human and animal tumors. Proteinase inhibitors may therefore be useful as therapeutic agents in anti-invasive and anti-metastatic treatment. The aims of this study were (1) to estimate the relative importance of proteinases in B16 cell invasion in vitro using synthetic, class-specific proteinase inhibitors and (2) to assess the inhibitory effect of some naturally occurring cysteine proteinase inhibitors. Serine proteinase inhibitor reduced invasiveness by up to 24%, whereas inhibition of aspartic proteinases reduced invasion by 11%. Synthetic inhibitors of cysteine proteinases markedly impaired invasion: cathepsin B inhibitors, particularly Ca-074Me, inhibited invasion from 20-40%, whereas cathepsin L inhibitor Clik 148 reduced invasion by 11%. The potato cysteine proteinase inhibitor PCPI 8.7 inhibited invasion by 21%, whereas another potato inhibitor, PCPI 6.6, and the mushroom cysteine proteinase inhibitor clitocypin had no effects. As the inhibitors that inhibited cathepsin B were in general more efficient at impairing the invasiveness, we conclude that of the two cysteine proteinases, cathepsin B plays a more important role than cathepsin L in murine melanoma cell invasion. https://greenmedinfo.com/article/proteinase-inhibitor-potato-exhibits-inhibitory-activity-against-melanoma-cell#comments Cancer Metastasis Melanoma Melanoma: Metastatic Potato Anti-metastatic Serine Proteinase Inhibitors Fungal Proteins In Vitro Study Sat, 02 Oct 2010 20:09:48 +0000 greenmedinfo 57300 at https://greenmedinfo.com Aloe-emodin significantly reduced the proliferation, stemness and invasive potential of melanospheres. https://greenmedinfo.com/article/aloe-emodin-significantly-reduced-proliferation-stemness-and-invasive-potentia PMID:  Eur J Pharmacol. 2015 Jun 3 ;762:283-292. Epub 2015 Jun 3. PMID: 26048310 Abstract Title:  Aloe-emodin exerts a potent anticancer and immunomodulatory activity on BRAF-mutated human melanoma cells. Abstract:  Aim of this study was to extend the knowledge on the antineoplastic effect of aloe-emodin (AE), a natural hydroxyanthraquinone compound, both in metastatic human melanoma cell lines and in primary stem-like cells (melanospheres). Treatment with AE caused reduction of cell proliferation and induction of SK-MEL-28 and A375 cells differentiation, characterized by a marked increase of transamidating activity of transglutaminase whose expression remained unmodified. In vitro antimetastatic property of AE was evaluated by adhesion and Boyden chamber invasion assays. The effect of AE on melanoma cytokines/chemokines production was determined by a multiplex assay: interestingly AE showed an immunomodulatory activity through GM-CSF and IFN-γ production. We report also that AE significantly reduced the proliferation, stemness and invasive potential of melanospheres. Moreover, AE treatment significantly enhanced dabrafenib (a BRAF inhibitor) antiproliferative activity in BRAF mutant cell lines. Our results confirm that AE possesses remarkable antineoplastic properties against melanoma cells, indicating this anthraquinone as a promising agent for differentiation therapy of cancer, or as adjuvant in chemotherapy and targeted therapy. Further, its mechanisms of action support a potential efficacy of AE treatment to counteract resistance of BRAF-mutated melanoma cells to target therapy. https://greenmedinfo.com/article/aloe-emodin-significantly-reduced-proliferation-stemness-and-invasive-potentia#comments Emodin Melanoma Melanoma: Metastatic Anti-metastatic Antiproliferative Chemotherapeutic Immunomodulatory Natural Substance/Drug Synergy In Vitro Study Wed, 24 Jun 2015 20:37:21 +0000 greenmedinfo 118439 at https://greenmedinfo.com Anthocyanin determination in blueberry extracts from various cultivars and their antiproliferative and apoptotic properties in B16-F10 metastatic murine melanoma cells. https://greenmedinfo.com/article/anthocyanin-determination-blueberry-extracts-various-cultivars-and-their-antip PMID:  Phytochemistry. 2013 Jul 24. Epub 2013 Jul 24. PMID: 23890760 Abstract Title:  Anthocyanin determination in blueberry extracts from various cultivars and their antiproliferative and apoptotic properties in B16-F10 metastatic murine melanoma cells. Abstract:  Blueberry consumption is associated with health benefits contributing to a reduced risk for cardiovascular disease, diabetes and cancer. The aim of this study was to determine the anthocyanin profile of blueberry extracts and to evaluate their effects on B16-F10 metastatic melanoma murine cells. Seven blueberry cultivars cultivated in Romania were used. The blueberry extracts were purified over an Amberlite XAD-7 resin and a Sephadex LH-20 column, in order to obtain the anthocyanin rich fractions (ARF). The antioxidant activity of the ARF of all cultivars was evaluated by ABTS, CUPRAC and ORAC assays. High performance liquid chromatography followed by electrospray ionization mass spectrometry (HPLC-ESI-MS) was used to identify and quantify individual anthocyanins. The anthocyanin content of tested cultivars ranged from 101.88 to 195.01mg malvidin-3-glucoside/100g fresh weight. The anthocyanin rich-fraction obtained from cultivar Torro (ARF-T) was shown to have the highest anthocyanin content and antioxidant activity, and inhibited B16-F10 melanoma murine cells proliferation at concentrations higher than 500μg/ml. In addition, ARF-T stimulated apoptosis and increased total LDH activity in metastatic B16-F10 melanoma murine cells. These results indicate that the anthocyanins from blueberry cultivar could be used as a chemopreventive or adjuvant treatment for metastasis control. https://greenmedinfo.com/article/anthocyanin-determination-blueberry-extracts-various-cultivars-and-their-antip#comments Anthocyanins Blueberry Cancer Metastasis Melanoma Melanoma: Metastatic Anti-metastatic In Vitro Study Mon, 12 Aug 2013 17:43:37 +0000 greenmedinfo 109399 at https://greenmedinfo.com Blueberry anthocyanin and anthocyanidin extracts inhibit the proliferation and trigger the apoptosis of B16-F10 cells. https://greenmedinfo.com/article/blueberry-anthocyanin-and-anthocyanidin-extracts-inhibit-proliferation-and-tri PMID:  Food Nutr Res. 2017 ;61(1):1325308. Epub 2017 Jun 19. PMID: 28680383 Abstract Title:  Antiproliferative and proapoptotic activities of anthocyanin and anthocyanidin extracts from blueberry fruits on B16-F10 melanoma cells. Abstract:  : Anthocyanins have been proven to affect multiple cancer-associated processes in different cancer cell lines. However, relatively few studies have investigated the effects of blueberry anthocyanins on metastatic melanoma. Thus, this study focuses on evaluating the chemopreventive potential of blueberry anthocyanins and their aglycones (anthocyanidins) in B16-F10 melanoma cells.: Blueberry anthocyanin and anthocyanidin extracts were prepared mainly by combined chromatography techniques. Their antiproliferative and proapoptotic effects on B16-F10 cells were evaluated by MTT assay, calcein acetoxymethyl ester/propidium iodide (calcein-AM/PI) staining, and flow cytometry of the cell cycle and apoptosis.: The MTT and calcein-AM/PI staining results showed that both anthocyanin (purity of 62.5%) and anthocyanidin (75.1%) extracts could significantly inhibit the viability and proliferation of B16-F10 cells in a dose-dependent manner, while anthocyanidin extracts exhibited significantly higher ( <p><a href="https://greenmedinfo.com/article/blueberry-anthocyanin-and-anthocyanidin-extracts-inhibit-proliferation-and-tri" target="_blank">read more</a></p> https://greenmedinfo.com/article/blueberry-anthocyanin-and-anthocyanidin-extracts-inhibit-proliferation-and-tri#comments Anthocyanins Blueberry Melanoma: Metastatic Antiproliferative Apoptotic In Vitro Study Fri, 09 Nov 2018 18:56:46 +0000 greenmedinfo 173660 at https://greenmedinfo.com Complete spontaneous regression of a metastatic melanoma of the mandible. https://greenmedinfo.com/article/complete-spontaneous-regression-metastatic-melanoma-mandible PMID:  Int J Oral Maxillofac Surg. 2018 Dec ;47(12):1519-1522. Epub 2018 Jun 30. PMID: 29970290 Abstract Title:  Complete spontaneous regression of a metastatic melanoma of the mandible: a case report and follow-up recommendations. Abstract:  Regression of metastatic melanoma is very rare and occurs in only 0.23% of cases. Metastasis to the oral cavity is particularly uncommon and accounts for only 1-3% of all oral malignancies. This report presents a case of spontaneous and complete regression of a metastatic melanoma in the mandibular ramus. The patient remains asymptomatic more than 2 years after diagnosis. The patient was followed up regularly. It is recommended that further surveillance imaging be performed in asymptomatic patients following discussion with the surgical and oncological teams. This type of surveillance, together with new systemic treatments, is advocated due to its potential to increase long-term survival even after relapse. <p><a href="https://greenmedinfo.com/article/complete-spontaneous-regression-metastatic-melanoma-mandible" target="_blank">read more</a></p> https://greenmedinfo.com/article/complete-spontaneous-regression-metastatic-melanoma-mandible#comments Melanoma: Metastatic Spontaneous Tumor Regression Human: Case Report Mon, 22 Apr 2019 14:08:26 +0000 greenmedinfo 185708 at https://greenmedinfo.com D-limonene ihibits the metastatic progression of melanoma cells in mice. https://greenmedinfo.com/article/d-limonene-ihibits-metastatic-progression-melanoma-cells-mice PMID:  J Exp Clin Cancer Res. 2003 Sep;22(3):419-24. PMID: 14582701 Abstract Title:  Effect of naturally occurring monoterpenes carvone, limonene and perillic acid in the inhibition of experimental lung metastasis induced by B16F-10 melanoma cells. Abstract:  The effects of naturally occurring monoterpenes on lung metastasis induced by B16F-10 melanoma cells were studied in C57BL/6 mice. Administration of monoterpenes such as limonene (100 micromoles/kg body wt. 10 doses i.p.) and perillic acid (50 micromoles/kg body wt 10 doses i.p.) remarkably reduced the metastatic tumour nodule formation by 65% and 67%, respectively. These results correlated with the biochemical parameters such as serum sialic acid, lung collagen hydroxyproline and uronic acid contents. Serum sialic acid level in control group was 126.8 microg/ml serum which was significantly lowered in limonene (49.3 microg/ml serum) and perillic acid treated animals (53.6 microg/ml serum). Uronic acid level was also inhibited to 56% and 39.7% in limonene and perillic acid treated animals, respectively. Histopathological studies also correlated with these above results. Administration of Carvone even at 100 micromoles/kg body wt. did not have any significant effect on the metastatic tumour growth. These results indicate that limonene and perillic acid could inhibit the metastatic progression of B16F-10 melanoma cells in mice. https://greenmedinfo.com/article/d-limonene-ihibits-metastatic-progression-melanoma-cells-mice#comments D-Limonene Malignant Melanoma Melanoma: Metastatic Animal Study Sun, 12 Jul 2009 02:42:43 +0000 greenmedinfo 45380 at https://greenmedinfo.com Deadly Chemo-Drug Costs 4,000x More Than Gold https://greenmedinfo.com/blog/deadly-chemo-drug-costs-4000x-more-gold <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2016<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/Sayer Ji/images/51059034_m.jpg" style="width: 632px; height: 758px;" /></p> <p><strong><span style="font-size:18px;"><em>One of the oldest marketing tricks in the book is to dramatically overprice something in order to increase its perceived value. Ironically, the less intrinsic value the commodity holds, the more effective such a tactic can be. This could explain what's going on with one of the highest priced and most useless chemotherapy drugs on the market today. </em></span></strong></p> <p dir="ltr">The chemotherapy agent is known as ipilimumab (trade name YERVOY®), and costs about $120,000 for a full course of treatment. While the manufacturer advertises&nbsp;<span style="font-size: 14.4px; line-height: 24.48px;">YERVOY®</span>&nbsp;as providing tangible hope to those with non-resectable&nbsp;or metastatic melanoma, it also boldly warns on its website that the effects of this drug can be quite deadly:</p><p><a href="https://greenmedinfo.com/blog/deadly-chemo-drug-costs-4000x-more-gold" target="_blank">read more</a></p> https://greenmedinfo.com/blog/deadly-chemo-drug-costs-4000x-more-gold#comments Cancer Metastasis Cancer Stem Cells Malignant Melanoma Melanoma Melanoma: Metastasis Melanoma: Metastatic Cancer Health Guide: Breast Cancer Sun, 20 Mar 2016 21:30:48 +0000 Sayer Ji 125007 at https://greenmedinfo.com Diosmin has a synergistic effect with interferon-alpha in inhibiting metastatic pulmonary melanoma. https://greenmedinfo.com/article/diosmin-has-synergistic-effect-interferon-alpha-inhibiting-metastatic-pulmonar PMID:  Cancer Biother Radiopharm. 2009 Jun;24(3):347-52. PMID: 19538057 Abstract Title:  Synergistic effect of diosmin and interferon-alpha on metastatic pulmonary melanoma. Abstract:  Melanoma is the most important skin cancer in terms of mortality, besides developing metastasis in about a third of the patients. Interferon-alpha (IFN-alpha), a highly toxic cytokine, tends to be one of the most important treatments for melanoma. Much effort is being directed at obtaining less-toxic antitumoral compounds, particularly natural compounds such as flavonoids. Our aim was to study the combined treatment of metastatic lung melanoma with IFN-alpha and diosmin in a murine model. In this article, we report the effect of a combined treatment of IFN-alpha and the flavonoid, diosmin, on a murine model of metastatic B16F10 melanoma, assessed both macroscopically (counting subpleural nodules) and microscopically by image analysis (calculating three indices). IFN-alpha showed a dose-dependent anti-invasive and antiproliferative activity in our study, while diosmin showed an anti-invasive activity similar to the lower dose of IFN-alpha used. However, the most relevant result was the synergistic antiproliferative effect shown by the combination of the flavonoid and the lowest dose of IFN-alpha, which was similar to that produced by the highest dose of the cytokine alone. https://greenmedinfo.com/article/diosmin-has-synergistic-effect-interferon-alpha-inhibiting-metastatic-pulmonar#comments Diosmin Melanoma Melanoma: Metastatic Antiproliferative Drug-Plant-Vitamin Synergies Animal Study Wed, 09 Sep 2009 22:16:15 +0000 greenmedinfo 46963 at https://greenmedinfo.com Gamma-tocotrienol has broad therapeutic activity against human melanoma cells. https://greenmedinfo.com/article/gamma-tocotrienol-has-broad-therapeutic-activity-against-human-melanoma-cells PMID:  Nutr Cancer. 2009;61(3):357-66. PMID: 19373609 Abstract Title:  Evidence of gamma-tocotrienol as an apoptosis-inducing, invasion-suppressing, and chemotherapy drug-sensitizing agent in human melanoma cells. Abstract:  To date, the most effective cure for metastatic melanoma remains the surgical resection of the primary tumor. Recently, tocotrienol-rich-fraction has shown antiproliferative effect on cancer cells. To elucidate this anticancer property in malignant melanoma, this study aimed, first, to identify the most potent isomer for eliminating melanoma cells and second to decipher the molecular pathway responsible for its activity. Results showed that the inhibitory effect of gamma-tocotrienol was most potent, which resulted in induction of apoptosis as evidenced by activation of procaspases and the accumulation of sub-G1 cell population. Examination of the prosurvival genes revealed that the gamma-tocotrienol-induced cell death was associated with suppression of NF-kappaB, EGF-R, and Id family proteins. Meanwhile, gamma-tocotrienol treatment also resulted in induction of JNK signaling pathway, and inhibition of JNK activity by selective inhibitor was able to partially block the effect of gamma-tocotrienol. Interestingly, gamma-tocotrienol treatment led to suppression of mesenchymal markers and the restoration of E-cadherin and gamma-catenin expression, which was associated with suppression of cell invasion capability. Furthermore, synergistic effect was observed when cells were cotreated with gamma-tocotrienol and chemotherapy drugs. Together, our results demonstrated for the first time the anti-invasion and chemonsensitization effect of gamma-tocotrienol against human malignant melanoma cells. https://greenmedinfo.com/article/gamma-tocotrienol-has-broad-therapeutic-activity-against-human-melanoma-cells#comments Malignant Melanoma Melanoma Melanoma: Metastatic Tocotrienol: Gamma Tocotrienols Vitamin E: Gamma-tocotrienol Drug-Plant-Vitamin Synergies In Vitro Study Fri, 22 May 2009 01:47:27 +0000 greenmedinfo 44402 at https://greenmedinfo.com Gomisin A has the potential to reduce metastatic melanoma through its anti-proliferative and anti-metastatic effects. https://greenmedinfo.com/article/gomisin-has-potential-reduce-metastatic-melanoma-through-its-anti-proliferativ PMID:  Phytomedicine. 2019 Dec 7 ;68:153147. Epub 2019 Dec 7. PMID: 32028184 Abstract Title:  Gomisin A ameliorates metastatic melanoma by inhibiting AMPK and ERK/JNK-mediated cell survival and metastatic phenotypes. Abstract:  BACKGROUND: Gomisin A (G.A), a lignan compound extracted from the fruits of Schisandra chinensis, is known to exert anti-tumor effects on hepatocarcinoma and colorectal cancer cells. Suppression of proliferation and metastatic abilities of cancer cells are some effective cancer treatment methods.PURPOSE: The objective of this study is to investigate the effects of G.A on metastatic melanoma, and the mechanism by which it affects metastatic melanoma.STUDY DESIGN: The anti-proliferative and anti-metastatic effects of G.A were observed in in vitro and in vivo.METHODS: WST assay and flow cytometry were conducted to investigate the effect of G.A on proliferation, cell cycle arrest, and apoptosis in metastatic melanoma cell lines. Migration and invasion abilities of G.A-treated melanoma cells were observed by wound healing and invasion assays.RESULTS: G.A (25-100 μM) decreased the viability of melanoma cells by inducing cell cycle arrest and apoptosis. These anti-proliferative effects of G.A were found to be mediated by AMPK, ERK, and JNK activation. G.A (5-20 μM) decreased the migration and invasion of melanoma cells by suppressing epithelial-mesenchymal transition (EMT). Consequently, G.A (2-50 mg/kg) inhibited lung metastasis by suppressing EMT and inducing cell cycle arrest and apoptosis in melanoma cells.CONCLUSION: These results conclude that G.A has the potential to reduce metastatic melanoma through its anti-proliferative and anti-metastatic effects. <p><a href="https://greenmedinfo.com/article/gomisin-has-potential-reduce-metastatic-melanoma-through-its-anti-proliferativ" target="_blank">read more</a></p> https://greenmedinfo.com/article/gomisin-has-potential-reduce-metastatic-melanoma-through-its-anti-proliferativ#comments Melanoma: Metastatic Schisandra Anti-metastatic Antiproliferative Animal Study In Vitro Study Sat, 14 Mar 2020 17:41:05 +0000 greenmedinfo 216055 at https://greenmedinfo.com Grape seed extract, red wine and especially diosmin, inhibit pulmonary metastatic melanoma. https://greenmedinfo.com/article/grape-seed-extract-red-wine-and-especially-diosmin-inhibit-pulmonary-metastati PMID:  Histol Histopathol. 2005 Oct;20(4):1121-9. PMID: 16136495 Abstract Title:  The effect of the flavonoid diosmin, grape seed extract and red wine on the pulmonary metastatic B16F10 melanoma. Abstract:  OBJECTIVE: To study the effect of different phenolic compounds and red wine on pulmonary metastatic melanoma. METHODS: Swiss mice were inoculated with 500000 melanocytes B16F10 and given oral doses of diosmin, grape seed extract (GSE) and red wine. A macroscopic count was made of the metastatic nodules on the lung surface and a microscopic study by image analysis of five sections, calculating the implantation percentage and tumoral growth and invasion indices. RESULTS: Macroscopically, the group treated with diosmin showed the greatest reduction (52%) in the number of metastatic nodules compared with the control group, which was treated with ethanol, while GSE and red wine caused decreases of 26.07 and 28.81%, respectively. Microscopically, there was a decrease in the implantation percentage after the administration of diosmin (79.4%) and red wine (20.19%), and an increase of 2.12% after the administration of GSE, all relative to the ethanol-treated control. As regards the growth index, diosmin produced a reduction of 67.44% and red wine a reduction of 20.62%, while GSE again produced an increase (25.33%). The reductions in the invasion index were 45.23, 31.65 and 17.57% with diosmin, GSE and red wine, respectively. CONCLUSIONS: Diosmin originated the greatest reduction in pulmonary metastases, both at the macroscopic and microscopic levels. https://greenmedinfo.com/article/grape-seed-extract-red-wine-and-especially-diosmin-inhibit-pulmonary-metastati#comments Diosmin Grape Seed Extract Lung Cancer Melanoma: Metastatic Pulmonary Cancer Red Wine Extract In Vitro Study Mon, 27 Jul 2009 13:11:54 +0000 greenmedinfo 46128 at https://greenmedinfo.com Highl doses of ascorbate up regulated 32 miRNAs 4-fold, mainly involved in tumor suppression and drug resistance. https://greenmedinfo.com/article/highl-doses-ascorbate-regulated-32-mirnas-4-fold-mainly-involved-tumor-suppres PMID:  Front Oncol. 2014 ;4:227. Epub 2014 Aug 25. PMID: 25202679 Abstract Title:  Epigenetic impacts of ascorbate on human metastatic melanoma cells. Abstract:  In recent years, increasing evidence has emerged demonstrating that high-dose ascorbate bears cytotoxic effects on cancer cells in vitro and in vivo, making ascorbate a pro-oxidative drug that catalyzes hydrogen peroxide production in tissues instead of acting as a radical scavenger. This anticancer effect of ascorbate is hypoxia-inducible factor-1α- and O2-dependent. However, whether the intracellular mechanisms governing this effect are modulated by epigenetic phenomena remains unknown. We treated human melanoma cells with physiological (200 μM) or pharmacological (8 mM) ascorbate for 1 h to record the impact on DNA methyltransferase (DNMT)-activity, histone deacetylases (HDACs), and microRNA (miRNA) expression after 12 h. The results were analyzed with the MIRUMIR online tool that estimates the power of miRNA to serve as potential biomarkers to predict survival of cancer patients. FACS cell-cycle analyses showed that 8 mMascorbate shifted BLM melanoma cells toward the sub-G1 fraction starting at 12 h after an initial primary G2/M arrest, indicative for secondary apoptosis induction. In pharmacological doses, ascorbate inhibited the DNMT activity in nuclear extracts of MeWo and BLM melanoma cells, but did not inhibit human HDAC enzymes of classes I, II, and IV. The expression of 151 miRNAs was altered 12 h after ascorbate treatment of BLM cells in physiological or pharmacological doses. Pharmacological doses up-regulated 32 miRNAs (≥4-fold) mainly involved in tumor suppression and drug resistance in ourpreliminary miRNA screening array. The most prominently up-regulated miRNAs correlated with a significantly increased overall survival of breast cancer or nasopharyngeal carcinoma patients of the MIRUMIR database with high expression of the respective miRNA. Our results suggest a possible epigeneticsignature of pharmacological doses of ascorbate in human melanoma cells and support further pre-clinical and possibly even clinical evaluation of ascorbate for melanoma therapy. https://greenmedinfo.com/article/highl-doses-ascorbate-regulated-32-mirnas-4-fold-mainly-involved-tumor-suppres#comments Melanoma Melanoma: Metastatic Vitamin C Anti-metastatic Apoptotic Cell cycle arrest MicroRNA modulator DNA Methyltransferase Epigenetic Modification In Vitro Study Thu, 02 Jul 2015 02:08:19 +0000 greenmedinfo 118642 at https://greenmedinfo.com Initial vitamin D deficiency and insufficient repletion is associated with a worse prognosis in patients with metastatic melanoma. https://greenmedinfo.com/article/initial-vitamin-d-deficiency-and-insufficient-repletion-associated-worse-progn PMID:  Oncotarget. 2017 Jan 24 ;8(4):6873-6882. PMID: 28036288 Abstract Title:  Vitamin D deficiency is associated with a worse prognosis in metastatic melanoma. Abstract:  Vitamin D deficiency (≤20 ng/mL) is associated with an increased incidence and worse prognosis of various types of cancer including melanoma. A retrospective, single-center study of individuals diagnosed with melanoma from January 2007 through June 2013 who had a vitamin D (25(OH)D3) level measured within one year of diagnosis was performed to determine whether vitamin D deficiency and repletion are associated with melanoma outcome. A total of 409 individuals diagnosed with histopathology-confirmed melanoma who had an ever measured serum 25(OH)D3 level were identified. 252 individuals with a 25(OH)D3 level recorded within one year after diagnosis were included in the study and the individual and melanoma characteristics such as age, sex, Breslow thickness, ulceration, stage, mitotic rate, and LDH were obtained from the medical record. A worse melanoma prognosis was associated with vitamin D deficiency (P=0.012), higher stage (P20 ng/mL increase. Our results suggest that initial vitamin D deficiency and insufficient repletion is associated with a worse prognosis in patients with metastatic melanoma. <p><a href="https://greenmedinfo.com/article/initial-vitamin-d-deficiency-and-insufficient-repletion-associated-worse-progn" target="_blank">read more</a></p> https://greenmedinfo.com/article/initial-vitamin-d-deficiency-and-insufficient-repletion-associated-worse-progn#comments Melanoma: Metastatic Vitamin D Deficiency Increased Risk Human Study Fri, 21 Jul 2017 00:53:41 +0000 greenmedinfo 150749 at https://greenmedinfo.com Kaempferol impairs aerobic glycolysis against melanoma metastasis. https://greenmedinfo.com/article/kaempferol-impairs-aerobic-glycolysis-against-melanoma-metastasis PMID:  Eur J Pharmacol. 2022 Sep 15 ;931:175226. Epub 2022 Aug 22. PMID: 36007607 Abstract Title:  Kaempferol impairs aerobic glycolysis against melanoma metastasis via inhibiting the mitochondrial binding of HK2 and VDAC1. Abstract:  Metastasis is the leading cause of death in melanoma patients. Aerobic glycolysis is a common metabolic feature in tumor and is closely related to cell growth and metastasis. Kaempferol (KAM) is one of the active ingredients in the total flavonoids of Chinese traditional medicine Sparganii Rhizoma. Studies have shown that it interferes with the cell cycle, apoptosis, angiogenesis and metastasis of tumor cells, but whether it can affect the aerobic glycolysis of melanoma is still unclear. Here, we explored the effects and mechanisms of KAM on melanoma metastasis and aerobic glycolysis. KAM inhibited the migration and invasion of A375 and B16F10 cells, and reduced the lung metastasis of melanoma cells. Extracellular acidification rates (ECAR) and glucose consumption were obviously suppressed by KAM, as well as the production of ATP, pyruvate and lactate. Mechanistically, the activity of hexokinase (HK), the first key kinase of aerobic glycolysis, was significantly inhibited by KAM. Although the total protein expression of HK2 was not significantly changed, the binding of HK2 and voltage-dependent anion channel 1 (VDAC1) on mitochondria was inhibited by KAM through AKT/GSK-3β signal pathway. In conclusion, KAM inhibits melanoma metastasis via blocking aerobic glycolysis of melanoma cells, in which the binding of HK2 and VDAC1 on mitochondria was broken. <p><a href="https://greenmedinfo.com/article/kaempferol-impairs-aerobic-glycolysis-against-melanoma-metastasis" target="_blank">read more</a></p> https://greenmedinfo.com/article/kaempferol-impairs-aerobic-glycolysis-against-melanoma-metastasis#comments Kaempferol Melanoma: Metastatic Anti-metastatic Antiproliferative Cancer Glycolysis In Vitro Study Thu, 08 Sep 2022 20:25:11 +0000 greenmedinfo 263116 at https://greenmedinfo.com Mangiferin may be a potential therapeutic agent with a new mechanism of targeting NIK for the treatment of metastatic melanoma. https://greenmedinfo.com/article/mangiferin-may-be-potential-therapeutic-agent-new-mechanism-targeting-nik-trea PMID:  Toxicol Appl Pharmacol. 2016 Sep 1 ;306:105-12. Epub 2016 Jul 11. PMID: 27417526 Abstract Title:  Mangiferin, a novel nuclear factor kappa B-inducing kinase inhibitor, suppresses metastasis and tumor growth in a mouse metastatic melanoma model. Abstract:  Advanced metastatic melanoma, one of the most aggressive malignancies, is currently without reliable therapy. Therefore, new therapies are urgently needed. Mangiferin is a naturally occurring glucosylxanthone and exerts many beneficial biological activities. However, the effect of mangiferin on metastasis and tumor growth of metastatic melanoma remains unclear. In this study, we evaluated the effect of mangiferin on metastasis and tumor growth in a mouse metastatic melanoma model. We found that mangiferin inhibited spontaneous metastasis and tumor growth. Furthermore, mangiferin suppressed the nuclear translocation of nuclear factor kappa B (NF-κB) and expression of phosphorylated NF-κB-inducing kinase (NIK), inhibitor of kappa B kinase (IKK), and inhibitor of kappa B (IκB) and increases the expression of IκB protein in vivo. In addition, we found that mangiferin inhibited the expression of matrix metalloproteinases (MMPs) and very late antigens (VLAs) in vivo. Mangiferin treatment also increased the expression of cleaved caspase-3, cleaved Poly ADP ribose polymerase-1 (PARP-1), p53 upregulated modulator of apoptosis (PUMA), p53, and phosphorylated p53 proteins, and decreased the expression of Survivin and Bcl-associated X (Bcl-xL) proteins in vivo. These results indicate that mangiferin selectivity suppresses the NF-κB pathway via inhibition of NIK activation, thereby inhibiting metastasis and tumor growth. Importantly, the number of reported NIK selective inhibitors is limited. Taken together, our data suggest that mangiferin may be a potential therapeutic agent with a new mechanism of targeting NIK for the treatment of metastatic melanoma. https://greenmedinfo.com/article/mangiferin-may-be-potential-therapeutic-agent-new-mechanism-targeting-nik-trea#comments Mangiferin Melanoma: Metastatic Anti-metastatic Apoptotic NF-kappaB Inhibitor Tumor Suppressor Protein p53 Upregulation In Vitro Study Fri, 02 Sep 2016 20:44:10 +0000 greenmedinfo 134574 at https://greenmedinfo.com