Corneal Neovascularization https://greenmedinfo.com/taxonomy/term/6302/all en A nutrient mixture containing lysine, proline, ascorbic acid, and green tea extract inhibits corneal neovascularization. https://greenmedinfo.com/article/nutrient-mixture-containing-lysine-proline-ascorbic-acid-and-green-tea-extract PMID:  Arch Med Res. 2007 Oct;38(7):789-91. Epub 2007 Jun 21. PMID: 17845900 Abstract Title:  Inhibition of corneal neovascularization with a nutrient mixture containing lysine, proline, ascorbic acid, and green tea extract. Abstract:  Corneal neovascularization is a significant, sight-threatening complication of many ocular surface disorders. Various growth factors and proteinases are involved in corneal neovascularization. The data supporting a causal role for vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs) are extensive. Inhibition of VEGF and MMPs is a main strategy for treating corneal neovascularization. Several findings have shown that corneal neovascularization can be reduced by using anti-VEGF and anti-MMPs agents. Efficacy of a nutrient mixture (NM) containing lysine, proline, ascorbic acid, and green tea extract has been demonstrated for reducing VEGF and MMPs secretion by various cells. Moreover, NM can inhibit endothelial cell migration and capillary tube formation. We herein note that topical application of NM is potentially useful for inhibiting corneal neovascularization and restoration of corneal clarity. Further investigations in animal models are needed to place NM alongside corneal neovascularization therapeutics. https://greenmedinfo.com/article/nutrient-mixture-containing-lysine-proline-ascorbic-acid-and-green-tea-extract#comments Corneal Neovascularization Green Tea Lysine Proline Vitamin C Matrix Metalloproteinases Inhibitors Vascular Endothelial Growth Factor A Inhibitor Plant Extracts In Vitro Study Sat, 13 Feb 2010 22:26:59 +0000 greenmedinfo 51321 at https://greenmedinfo.com Naringenin eye drops inhibit corneal neovascularization by anti-inflammatory and antioxidant mechanisms. https://greenmedinfo.com/article/naringenin-eye-drops-inhibit-corneal-neovascularization-anti-inflammatory-and- n/a PMID:  Invest Ophthalmol Vis Sci. 2017 11 1 ;58(13):5764-5776. PMID: 29117277 Abstract Title:  Naringenin Eye Drops Inhibit Corneal Neovascularization by Anti-Inflammatory and Antioxidant Mechanisms. Abstract:  Purpose: To investigate the effect of naringenin eye drops in corneal neovascularization induced by alkali (1 N NaOH) burn in mice. Methods: Corneal neovascularization in the right eye of male Swiss mice was induced by alkali. Treatment with naringenin eye drops (0.08-80μg; 8 μL of 0.01-10 g/L solution) or vehicle (saline) started 2 days before corneal neovascularization was induced and was performed twice a day. Mice were treated up until the time animals were euthanized and cornea tissue was collected for testing, which was 2, 4, and 6 hours after alkali stimulus for cytokine and antioxidant capacity measurements, and 3 and/or 7 days after alkali stimulus for the assessment of corneal epithelial thickness and neovascularization, neutrophil, and macrophage recruitment, and vascular endothelial growth factor (Vegf), platelet-derived growth factor (Pdgf), matrix metalloproteinase-14 (Mmp14), and pigment epithelium-derived factor (Pedf) mRNA expression. Results: Naringenin eye drops inhibited alkali burn-induced neutrophil (myeloperoxidase activity and recruitment of Lysm-GFP+ cells) and macrophage (N-acetyl-β-D glucosaminidase activity) recruitment into the eye, decrease in epithelial thickness, and neovascularization in the cornea. Further, naringenin inhibited alkali-induced cytokine (IL-1β and IL-6) production, Vegf, Pdgf, and Mmp14 mRNA expression, and the reduction of ferric reducing antioxidantpower and Azinobis-(3-Ethylbenzothiazoline 6-Sulfonic acid) radical scavenging capacity as well as increased the reduced glutathione and protein-bound sulfhydryl groups levels. Conclusions: Collectively, these results indicate that naringenin eye drops are protective in alkali-induced corneal burn by inhibiting leukocyte recruitment, the proangiogenic factor expression, inflammatory cytokine production, and loss of antioxidant defenses. https://greenmedinfo.com/article/naringenin-eye-drops-inhibit-corneal-neovascularization-anti-inflammatory-and-#comments Corneal Neovascularization Anti-Inflammatory Agents Antioxidants Interleukin-1 beta downregulation Interleukin-6 Downregulation Animal Study Tue, 30 Apr 2019 13:55:17 +0000 greenmedinfo 186533 at https://greenmedinfo.com Nattokinase attenuates retinal neovascularization. https://greenmedinfo.com/article/nattokinase-attenuates-retinal-neovascularization PMID:  Invest Ophthalmol Vis Sci. 2021 May 3 ;62(6):25. PMID: 34036312 Abstract Title:  Nattokinase Attenuates Retinal Neovascularization Via Modulation of Nrf2/HO-1 and Glial Activation. Abstract:  Purpose: Nattokinase (NK), an active ingredient extracted from traditional food Natto, has been studied for prevention and treatment of cardiovascular diseases due to various vasoprotective effects, including fibrinolytic, antihypertensive, anti-atherosclerotic, antiplatelet, and anti-inflammatory activities. Here, we reported an antineovascular effect of NK against experimental retinal neovascularization.Methods: The inhibitory effect of NK against retinal neovascularization was evaluated using an oxygen-induced retinopathy murine model. Expressions of Nrf2/HO-1 signaling and glial activation in the NK-treated retinae were measured. We also investigated cell proliferation and migration of human umbilical vein endothelial cells (HUVECs) after NK administration.Results: NK treatment significantly attenuated retinal neovascularization in the OIR retinae. Consistently, NK suppressed VEGF-induced cell proliferation and migration in a concentration-dependent manner in cultured vascular endothelial cells. NK ameliorated ischemic retinopathy partially via activating Nrf2/HO-1. In addition, NK orchestrated reactive gliosis and promoted microglial activation toward a reparative phenotype in ischemic retina. Treatment of NK exhibited no cell toxicity or anti-angiogenic effects in the normal retina.Conclusions: Our results revealed the anti-angiogenic effect of NK against retinal neovascularization via modulating Nrf2/HO-1, glial activation and neuroinflammation, suggesting a promising alternative treatment strategy for retinal neovascularization. <p><a href="https://greenmedinfo.com/article/nattokinase-attenuates-retinal-neovascularization" target="_blank">read more</a></p> https://greenmedinfo.com/article/nattokinase-attenuates-retinal-neovascularization#comments Corneal Neovascularization Nattokinase Retinal Diseases Antioxidants Heme oxygenase-1 up-regulation Nrf2 activation Animal Study Sun, 22 Aug 2021 02:22:09 +0000 greenmedinfo 244705 at https://greenmedinfo.com Theanine suppresses oxygen-induced retinal neovascularization associated in a rat model of retinopathy. https://greenmedinfo.com/article/theanine-suppresses-oxygen-induced-retinal-neovascularization-associated-rat-m PMID:  Nippon Ganka Gakkai Zasshi. 2008 Aug;112(8):669-73. PMID: 18767491 Abstract Title:  [The effect of theanine on oxygen-induced retinal neovascularization in the neonatal rat]. Abstract:  PURPOSE: This study was aimed to investigate the preventive effects of theanine treatment on a rat model of oxygen-induced ischemic retinopathy (OIR). METHODS: OIR was induced by maintaining the Sprague-Dawley neonatal rats in 80% oxygen. The rats were treated once daily with gastric gavage of theanine (5 or 50 mg/kg) or distilled water (DW) from postnatal days 6 to 17. The retinal neovascularization (NV) was scored and avascular areas(AVAs) were measured as a % of total retinal area (% AVAs) at day 18. RESULTS: The % AVAs in 5 mg/kg theanine (13.2 +/- 2.8%) and 50 mg/kg theanine (9.4 +/- 2.2%, p &lt; 0.05) treatment were lower than those in DW (18.9 +/- 2.9 %). The NV scores with 5 mg/kg theanine(4.2 +/- 0.5) or 50 mg/kg theanine (3.4 +/- 0.6) treatment were lower than those with DW (4.7 +/- 0.6). CONCLUSION: Theanine treatment suppresses the neovascularization in a rat model of OIR. These results suggest that theanine may prevent retinopathy of prematurity. https://greenmedinfo.com/article/theanine-suppresses-oxygen-induced-retinal-neovascularization-associated-rat-m#comments Corneal Neovascularization Retinopathy of Prematurity Retrolental Fibroplasia Theanine Animal Study Thu, 09 Jul 2009 02:28:19 +0000 greenmedinfo 45221 at https://greenmedinfo.com Topical application of curcumin was useful in reducing experimental corneal neovascularization and can be used to inhibit angiogenesis in the cornea. https://greenmedinfo.com/article/topical-application-curcumin-was-useful-reducing-experimental-corneal-neovascu PMID:  Curr Eye Res. 2010 Apr;35(4):274-80. PMID: 20373893 Abstract Title:  The effect of curcumin on corneal neovascularization in rabbit eyes. Abstract:  PURPOSE: To investigate the anti-angiogenic effect of topical curcumin on corneal neovascularization in a rabbit model.MATERIALS AND METHODS: One week after suturing, six eyes were treated with balanced salt solution (BSS) (group A), and six eyes were treated with curcumin 40, 80, or 160 micromol/L (groups B, C, and D, respectively), topically two times a day. After one week, light microscopy was used to analyze corneal neovascularization. The concentration of vascular endothelial growth factor (VEGF) mRNA in the corneal tissue was measured by reverse transcriptase-polymerase chain reaction (RT-PCR), and the activation of NF-kappaB was examined by immunofluorescent staining.RESULTS: Seven days after treatment, the sizes of the neovascularized areas were significantly reduced in groups B (50.1% +/- 6.7%), C (43.2% +/- 8.1%), and D (29.5% +/- 7.8%) compared with group A (69.5% +/- 1.5%) (p https://greenmedinfo.com/article/topical-application-curcumin-was-useful-reducing-experimental-corneal-neovascu#comments Corneal Neovascularization Curcumin Anti-Angiogenic NF-kappaB Inhibitor Vascular Endothelial Growth Factor A Inhibitor In Vitro Study Thu, 10 Feb 2011 22:43:51 +0000 greenmedinfo 61162 at https://greenmedinfo.com Z. multiflora could be introduced as a significant angiogenesis inhibitor for angiogenesis-dependent diseases https://greenmedinfo.com/article/z-multiflora-could-be-introduced-significant-angiogenesis-inhibitor-angiogenes PMID:  Int J Hematol Oncol Stem Cell Res. 2017 Apr 1 ;11(2):96-101. PMID: 28875003 Abstract Title:  Inhibition of Angiogenesis: A Novel Effect of Zataria Multiflora. Abstract:  Angiogenesis, the formation of new blood vessels from preexisting ones, is among the most important physiological and pathological processes that occur in the body. Under pathological conditions such as tumor growth, psoriasis, corneal neovascularization and rheumatoid arthritis, angiogenesis is substantial for the development of the disease. Zataria multiflora is a member of the Labiatae family with a vast range of traditional uses which has been long known and applied in Iran old medicine. The aim of this study was the evaluation of anti-angiogenic potential of Zataria multiflora.In this study, human umbilical vein endothelial cells (HUVECs) were isolated from newborn umbilical veins and then cultured for cytotoxicity (LDH test) assay. Regarding LDH results, following tests such as angiogenesis (cytodex-3 micro carrier) and migration (wound healing) tests were designed.The cytotoxicity assays showed no toxicity of Z.multiflora toward HUVECs in the range of 10-450µg/mL of the extract. This extract was also able to inhibit angiogenesis and migration at 200µg/mL.Our data clearly demonstrated an inhibitory effect of Z. multiflora on angiogenesis and migration of HUVECs. Z. multiflora could be introduced as a significant angiogenesis inhibitor for angiogenesis-dependent diseases in further complementary studies. <p><a href="https://greenmedinfo.com/article/z-multiflora-could-be-introduced-significant-angiogenesis-inhibitor-angiogenes" target="_blank">read more</a></p> https://greenmedinfo.com/article/z-multiflora-could-be-introduced-significant-angiogenesis-inhibitor-angiogenes#comments Cancers: All Corneal Neovascularization Psoriasis Rheumatoid Arthritis Zataria multiflora Angiogenesis Inhibitors Human In Vitro Mon, 11 Feb 2019 18:53:42 +0000 greenmedinfo 179463 at https://greenmedinfo.com