Pancreatic Cancer: Metastatic https://greenmedinfo.com/taxonomy/term/70804/all en "Anti-metastasis effect of thymoquinone on human pancreatic cancer" https://greenmedinfo.com/article/anti-metastasis-effect-thymoquinone-human-pancreatic-cancer PMID:  Yao Xue Xue Bao. 2011 Aug ;46(8):910-4. PMID: 22007514 Abstract Title:  [Anti-metastasis effect of thymoquinone on human pancreatic cancer]. Abstract:  Recent studies reported that thymoquinone (TQ), a component derived from the medicinal spice Nigella sativa (also called black cumin), exhibited inhibitory effects on cell proliferation of many cancer cell lines. This study was performed to investigate the anti-metastatic effect of thymoquinone on the pancreatic cancer in vitro and in vivo. The results showed that thymoquinone suppressed the migration and invasion of Panc-1 cells in a does-dependent manner. To investigate the possible mechanisms involved in these events, Western blotting analysis was performed, and found that thymoquinone significantly down-regulates NF-kappaB and MMP-9 in Panc-1 cells. In addition, metastatic model simulating human pancreatic cancer was established by orthotropic implantation of histologically intact pancreatic tumor tissue into the pancreatic wall of nude mice. And administration of thymoquinone significantly reduced tumor metastasis compared to untreated control. Furthermore, the expression of NF-kappaB and MMP-9 in tumor tissues was also suppressed after treatment with thymoquinone. Taken together, the results indicate that thymoquinone exerts anti-metastatic activity on pancreatic cancer both in vitro and in vivo, which may be related to down-regulation of NF-kappaB and its regulated molecules such as MMP-9 protein. Consequently, these results provide important insights into thymoquinone as an antimetastatic agent for the treatment of human pancreatic cancer. https://greenmedinfo.com/article/anti-metastasis-effect-thymoquinone-human-pancreatic-cancer#comments Cancer Metastasis Pancreatic Cancer: Metastatic Thymoquinone Anti-metastatic Animal Study Wed, 19 Dec 2012 19:51:04 +0000 greenmedinfo 87224 at https://greenmedinfo.com Efficacy of liposomal curcumin in a human pancreatic tumor xenograft model: inhibition of tumor growth and angiogenesis. https://greenmedinfo.com/article/efficacy-liposomal-curcumin-human-pancreatic-tumor-xenograft-model-inhibition- PMID:  Anticancer Res. 2013 Sep ;33(9):3603-9. PMID: 24023285 Abstract Title:  Efficacy of liposomal curcumin in a human pancreatic tumor xenograft model: inhibition of tumor growth and angiogenesis. Abstract:  BACKGROUND: Liposome-based drug delivery has been successful in the past decade, with some formulations being Food and Drug Administration (FDA)-approved and others in clinical trials around the world. The major disadvantage associated with curcumin, a potent anticancer agent, is its poor aqueous solubility and hence low systemic bioavailability. However, curcumin can be encapsulated into liposomes to improve systemic bioavailability.MATERIALS AND METHODS: We determined the antitumor effects of a liposomal curcumin formulation against human MiaPaCa pancreatic cancer cells both in vitro and in xenograft studies. Histological sections were isolated from murine xenografts and immunohistochemistry was performed.RESULTS: The in vitro (IC50) liposomal curcumin proliferation-inhibiting concentration was 17.5μM. In xenograft tumors in nude mice, liposomal curcumin at 20 mg/kg i.p. three-times a week for four weeks induced 42% suppression of tumor growth compared to untreated controls. A potent antiangiogenic effect characterized by a reduced number of blood vessels and reduced expression of vascular endothelial growth factor and annexin A2 proteins, as determined by immunohistochemistry was observed in treated tumors.CONCLUSION: These data clearly establish the efficacy of liposomal curcumin in reducing human pancreatic cancer growth in the examined model. The therapeutic curcumin-based effects, with no limiting side-effects, suggest that liposomal curcumin may be beneficial in patients with pancreatic cancer. https://greenmedinfo.com/article/efficacy-liposomal-curcumin-human-pancreatic-tumor-xenograft-model-inhibition-#comments Curcumin Curcumin: Lipidated Pancreatic Cancer Pancreatic Cancer: Metastatic Anti-metastatic Animal Study In Vitro Study Mon, 16 Sep 2013 15:27:34 +0000 greenmedinfo 109735 at https://greenmedinfo.com Emodin exerts anti-metastatic activity in pancreatic cancer both in vitro and in vivo. https://greenmedinfo.com/article/emodin-exerts-anti-metastatic-activity-pancreatic-cancer-both-vitro-and-vivo PMID:  Zhongguo Zhong Yao Za Zhi. 2011 Nov ;36(22):3167-71. PMID: 22375400 Abstract Title:  [Experimental study on anti-metastasis effect of emodin on human pancreatic cancer]. Abstract:  OBJECTIVE: To investigate the anti-metastasis effect of emodin on the pancreatic cancer in vitro and in vivo.METHOD: Human pancreatic cancer cell line SW1990 was treated with different concentrations of emodin (10, 20, 40 micromol x L(-1)) for 2 h, the effects of emodin on the migration and invasion of SW1990 cells were examined by using wound assay and matrigel counting. Western blot was used to detect the protein expression of NF-kappaB and MMP-9 in SW1990 cells after various concentrations of emodin (10, 20, 40 micromol x L(-1)) treatment for 48 h. Metastatic model simulating human pancreatic cancer was established by orthotropic implantation of histologically intact human tumor tissue into pancreatic wall of nude mice, and then divided into three groups: control group, low-dose emodin group (L-EMO) and high-dose emodin group (H-EMO). Eight weeks after implantation, the presences of metastasis were evaluated respectively after the mice were sacrificed. Immunohistochemistry was used to detect the positive expression of CD34, NF-kappaB and MMP-9 in the tumors.RESULT: Emodin suppressed the migration and invasion of SW1990 cells in a dose-dependent manner. Western bolt assay indicated that emodin down-regulated the expression of NF-kappaB and MMP-9 proteins in SW1990 cells. The incidences of metastasis were decreased significantly in L-EMO group and H-EMO group as compared with that in control group. The percentage of CD34, NF-kappaB and MMP-9-positive cells in the tumors were significantly reduced by the administration of emodin.CONCLUSION: Emodin exerts anti-metastatic activity in pancreatic cancer both in vitro and in vivo, which may be related to down-regulation of NF-kappaB and MMP-9. https://greenmedinfo.com/article/emodin-exerts-anti-metastatic-activity-pancreatic-cancer-both-vitro-and-vivo#comments Cancer Metastasis Emodin Pancreatic Cancer Pancreatic Cancer: Metastatic Anti-Angiogenic Anti-metastatic Matrix metalloproteinase-9 (MMP-9) inhibitor NF-kappaB Inhibitor Animal Study Fri, 30 Mar 2012 20:40:53 +0000 greenmedinfo 73739 at https://greenmedinfo.com Ginkgolic acid reduced the expression of the key enzymes involved in lipogenesis and restrained the tumor growth. https://greenmedinfo.com/article/ginkgolic-acid-reduced-expression-key-enzymes-involved-lipogenesis-and-restrai PMID:  Oncotarget. 2015 Mar 26. Epub 2015 Mar 26. PMID: 25895130 Abstract Title:  Ginkgolic acid suppresses the development of pancreatic cancer by inhibiting pathways driving lipogenesis. Abstract:  Ginkgolic acid (GA) is a botanical drug extracted from the seed coat of Ginkgo biloba L. with a wide range of bioactive properties, including anti-tumor effect. However, whether GA has antitumor effect on pancreatic cancer cells and the underlying mechanisms have yet to be investigated. In this study, we show that GA suppressed the viability of cancer cells but has little toxicity on normal cells, e.g, HUVEC cells. Furthermore, treatment of GA resulted in impaired colony formation, migration, and invasion ability and increased apoptosis of cancer cells. In addition, GA inhibited the de novo lipogenesis of cancer cells through inducing activation of AMP-activated protein kinase (AMPK) signaling and downregulated the expression of key enzymes (e.g. acetyl-CoA carboxylase [ACC], fatty acid synthase [FASN]) involved in lipogenesis. Moreover, the in vivo experiment showed that GA reduced the expression of the key enzymes involved in lipogenesis and restrained the tumor growth. Taken together, our results suggest that GA may serve as a new candidate against tumor growth of pancreatic cancer partially through targeting pathway driving lipogenesis. https://greenmedinfo.com/article/ginkgolic-acid-reduced-expression-key-enzymes-involved-lipogenesis-and-restrai#comments Ginkgo biloba Pancreatic Cancer Pancreatic Cancer: Metastatic Anti-metastatic Anticarcinogenic Agents Antiproliferative Chemopreventive Animal Study In Vitro Study Thu, 09 Jul 2015 15:59:22 +0000 greenmedinfo 118857 at https://greenmedinfo.com Kaempferol may be a safe inhibitor against malignant pancreatic cancers. https://greenmedinfo.com/article/kaempferol-may-be-safe-inhibitor-against-malignant-pancreatic-cancers PMID:  PLoS One. 2016 ;11(5):e0155264. Epub 2016 May 13. PMID: 27175782 Abstract Title:  Kaempferol Inhibits Pancreatic Cancer Cell Growth and Migration through the Blockade of EGFR-Related Pathway In Vitro. Abstract:  Pancreatic cancer is one of the most appalling cancers with a pessimistic prognosis. Despite many therapies, there has been no improvement of survival rates. In this study, we assessed the anti-cancer effects of kaempferol, a well known flavonoid having functional bio-activity against various malignant tumors. Kaempferol had anti-cancer effects on Miapaca-2, Panc-1, and SNU-213 human pancreatic cancer cells. In a dose-dependent manner, kaempferol decreased viability of these pancreatic cancer cells by increasing apoptosis. In particular, kaempferol effectively inhibited the migratory activity of human pancreatic cancer cells at relatively low dosages without any toxicity. The anti-cancer effect of kaempferol was mediated by inhibition of EGFR related Src, ERK1/2, and AKT pathways. These results collectively indicate that kaempferol, a phytochemical ingredient reported to have anti-viability and anti-oxidant properties, can act as a safety anti-migration reagent in human pancreatic cancer cells, which provide the rationale for further investigation of kaempferol as a strong candidate for the potential clinical trial of malignant pancreatic cancers. https://greenmedinfo.com/article/kaempferol-may-be-safe-inhibitor-against-malignant-pancreatic-cancers#comments Kaempferol Pancreatic Cancer Pancreatic Cancer: Metastatic Anti-metastatic Epidermal growth factor receptor (EGFR) inhibitor Dose Response In Vitro Study Tue, 31 May 2016 22:02:11 +0000 greenmedinfo 128156 at https://greenmedinfo.com These results demonstrate the anti-tumor effects of oil palm phenolics on pancreatic cancer cells. https://greenmedinfo.com/article/these-results-demonstrate-anti-tumor-effects-oil-palm-phenolics-pancreatic-can PMID:  Anticancer Res. 2015 Jan ;35(1):97-106. PMID: 25550539 Abstract Title:  Oil palm phenolics (OPP) inhibit pancreatic cancer cell proliferation via suppression of NF-κB pathway. Abstract:  BACKGROUND: Oil palm phenolics (OPP) or Palm Juice (PJ), a water soluble extract from the palm fruit (Elaies guineensis) has been documented to have anti-carcinogenic activities in various cancer types. MATERIALS AND METHODS: To investigate OPP effects in pancreatic cancer (PaCa) cells, two PaCa cell lines (PANC-1 and BxPC-3) were treated with different OPP doses. The anti-proliferative, apoptotic and anti-invasive properties of OPP were evaluated using MTS, cytoplasmic histone-DNA fragmentation and matrigel invasive assays, respectively. RESULTS: OPP suppressed PaCa proliferation in a dose-dependent manner. Its anti-invasive effects were validated by decreased expressions of MMP-9 and VEGF. Cell-cycle analysis demonstrated that cells were arrested in the S phase. OPP-induced apoptosis was associated with decrease in survivin and Bcl-XL expressions and increased expression of cleaved caspase-3, caspase-9 and PARP. CONCLUSION: Overall, our results demonstrate the anti-tumor effects of OPP on PaCa cells, providing initial evidence towards its potential therapeutic use. https://greenmedinfo.com/article/these-results-demonstrate-anti-tumor-effects-oil-palm-phenolics-pancreatic-can#comments Palm Oil Pancreatic Cancer Pancreatic Cancer: Metastatic Anti-metastatic Antiproliferative Apoptotic Cell cycle arrest NF-kappaB Inhibitor Dose Response Plant Oils In Vitro Study Fri, 10 Jul 2015 18:52:53 +0000 greenmedinfo 118909 at https://greenmedinfo.com α-mangostin suppressed the proliferation, migration, and invasion of pancreatic cancer cells. https://greenmedinfo.com/article/mangostin-suppressed-proliferation-migration-and-invasion-pancreatic-cancer-ce PMID:  Biomed Res Int. 2014 ;2014:546353. Epub 2014 Apr 10. PMID: 24812621 Abstract Title:  α-Mangostin suppresses the viability and epithelial-mesenchymal transition of pancreatic cancer cells by downregulating the PI3K/Akt pathway. Abstract:  α -Mangostin, a natural product isolated from the pericarp of the mangosteen fruit, has been shown to inhibit the growth of tumor cells in various types of cancers. However, the underlying molecular mechanisms are largely unclear. Here, we report that α -mangostin suppressed the viability and epithelial-mesenchymal transition (EMT) of pancreatic cancer cells through inhibition of the PI3K/Akt pathway. Treatment of pancreatic cancer BxPc-3 and Panc-1 cells with α -mangostin resulted in loss of cell viability, accompanied by enhanced cell apoptosis, cell cycle arrest at G1 phase, and decreaseof cyclin-D1. Moreover, Transwell and Matrigel invasion assays showed that α -mangostin significantly reduced the migration and invasion of pancreatic cancer cells. Consistent with these results, α -mangostin decreased the expression of MMP-2, MMP-9, N-cadherin, and vimentin and increased the expression of E-cadherin. Furthermore, we found that α -mangostin suppressed the activity of the PI3K/Akt pathway in pancreatic cancer cells as demonstrated by the reduction of the Akt phosphorylation by α -mangostin. Finally, α -mangostin significantly inhibited the growth of BxPc-3 tumor mouse xenografts. Our results suggest that α -mangostin may be potentially used as a novel adjuvant therapy or complementary alternative medicine for the management of pancreatic cancers. https://greenmedinfo.com/article/mangostin-suppressed-proliferation-migration-and-invasion-pancreatic-cancer-ce#comments Mangosteen Pancreatic Cancer Pancreatic Cancer: Metastatic Anti-metastatic Antiproliferative Apoptotic Cell cycle arrest PI3K/Akt/signaling suppression Animal Study In Vitro Study Fri, 26 Sep 2014 23:04:16 +0000 greenmedinfo 114862 at https://greenmedinfo.com