EGCG (Epigallocatechin gallate) https://greenmedinfo.com/category/keywords/egcg-epigallocatechin-gallate en EGCG ameliorates lethal graft versus host disease and reduces related target organ damage. https://greenmedinfo.com/article/egcg-ameliorates-lethal-graft-versus-host-disease-and-reduces-related-target-o n/a PMID:  PLoS One. 2017 ;12(1):e0169630. Epub 2017 Jan 19. PMID: 28103249 Abstract Title:  The Green Tea Catechin Epigallocatechin Gallate Ameliorates Graft-versus-Host Disease. Abstract:  Allogeneic hematopoetic stem cell transplantation (allo-HSCT) is a standard treatment for leukemia and other hematologic malignancies. The major complication of allo-HSCT is graft-versus-host-disease (GVHD), a progressive inflammatory illness characterized by donor immune cells attacking the organs of the recipient. Current GVHD prevention and treatment strategies use immune suppressive drugs and/or anti-T cell reagents these can lead to increased risk of infections and tumor relapse. Recent research demonstrated that epigallocatechin gallate (EGCG), a component found in green tea leaves at a level of 25-35% at dry weight, may be useful in the inhibition of GVHD due to its immune modulatory, anti-oxidative and anti-angiogenic capacities. In murine allo-HSCT recipients treated with EGCG, we found significantly reduced GVHD scores, reduced target organ GVHD and improved survival. EGCG treated allo-HSCT recipients had significantly higher numbers of regulatory T cells in GVHD target organs and in the blood. Furthermore, EGCG treatment resulted in diminished oxidative stress indicated by significant changes of glutathione blood levels as well as glutathione peroxidase in the colon. In summary, our study provides novel evidence demonstrating that EGCG ameliorates lethal GVHD and reduces GVHD-related target organ damage. Possible mechanisms are increased regulatory T cell numbers and reduced oxidative stress. https://greenmedinfo.com/article/egcg-ameliorates-lethal-graft-versus-host-disease-and-reduces-related-target-o#comments EGCG (Epigallocatechin gallate) Graft vs Host Disease Antioxidants Immunomodulatory Antioxidants EGCG (Epigallocatechin gallate) Graft vs Host Disease Hematopoietic Stem Cells Immunomodulatory Risk Reduction Animal Study Tue, 24 Jan 2017 00:44:41 +0000 greenmedinfo 142473 at https://greenmedinfo.com EGCG is effective in decreasing cell viability, apoptosis induction and enhancement of caspase 3 and Fas expression level in jurkat cells. https://greenmedinfo.com/article/egcg-effective-decreasing-cell-viability-apoptosis-induction-and-enhancement-c n/a PMID:  Indian J Hematol Blood Transfus. 2018 Apr ;34(2):253-260. Epub 2017 Aug 1. PMID: 29622866 Abstract Title:  The Inhibitory Effect of Epigallocatechin Gallate on the Viability of T Lymphoblastic Leukemia Cells is Associated with Increase of Caspase-3 Level and Fas Expression. Abstract:  Acute lymphoblastic leukemia is the most prevalent cancer in children. Novel components to help struggle aggressive malignancies and overcome some side effects of conventional treatments could be a promising strategy. Epigallocatechingallate (EGCG), have attracted the attention of scientists for prevention or treatment of some cancers. Jurkat cells were incubated with the different concentrations of EGCG (30-100 µm) for 24, 48, and 72 h and cell viability was investigated using MTS test. Apoptosis and the level of caspase 3 alterations were evaluated using flowcytometry and expression of Fas by Real Time PCR. EGCG decreased viability of cells with an inhibition concentration (IC50) of 82.8 ± 3.1, 68.8 ± 4 and 59.7 ± 4.8 μM in 24,48 and 72 h. 50, 70 and 100 µM concentrations of EGCG induced apoptosis in about 31, 40 and 71% of the cells, respectively. The mean value of caspase 3 positive cells in the presence of 50, 70 and 100 µm concentrations of EGCG was 19.3 ± 2.9, 29.5 ± 3.1 and 61.2 ± 3.4 respectively compared to 7.8 ± 1.1 in control with a significant difference at 100 µm concentration. Treatment with EGCG for 48 h enhanced the expression of Fas reaching to a significant level at 100 µM concentration. EGCG is effective in decrease cell viability, apoptosis induction and enhancement of caspase 3 andexpression level in jurkat cells. A comprehensive understanding of molecular events and pharmacokinetics of the component and experiments in animal models are required for dose determination and its interaction with other components of combination chemotherapy. https://greenmedinfo.com/article/egcg-effective-decreasing-cell-viability-apoptosis-induction-and-enhancement-c#comments EGCG (Epigallocatechin gallate) Leukemia: T-cell acute Lymphoblastic Antiproliferative Apoptotic Antiproliferative Apoptotic EGCG (Epigallocatechin gallate) Leukemia: T-cell acute Lymphoblastic In Vitro Study Wed, 30 May 2018 23:32:14 +0000 greenmedinfo 165021 at https://greenmedinfo.com EGCG protects PC12 cells from corticosterone induced neurotoxicity. https://greenmedinfo.com/article/egcg-protects-pc12-cells-corticosterone-induced-neurotoxicity n/a PMID:  Exp Ther Med. 2018 May ;15(5):4284-4290. Epub 2018 Mar 8. PMID: 29731823 Abstract Title:  (-)-Epigallocatechin-3-gallate protects PC12 cells against corticosterone-induced neurotoxicity via the hedgehog signaling pathway. Abstract:  It has been acknowledged that environmental stress is a risk factor for developing mental disorders. Chronic stress may contribute to the hyperactivation of the hypothalamic-pituitary-adrenal (HPA) axis and a sustained rise in the levels of glucocorticoids (GCs). A high concentration of corticosterone (CORT) damages neuronal PC12 cells. It has been reported that (-)-Epigallocatechin-3-gallate (EGCG), a major component of green tea, exhibits neuroprotective activity. However, the protective effect of EGCG on neuronal cells injured by CORT remains to be elucidated. The present study aimed to identify the effects of EGCG on CORT-injured neuronal PC12 cells and its associated mechanisms of action. CORT-injured PC12 cells were pretreated with EGCG with or without cyclopamine. Cell viability was assessed using an MTT assay, changes in cell morphology were observed using phase-contrast microscopy, cellular apoptosis was assessed by Hoechst 33342 staining, cell proliferation was measured using a cell counting kit-8 assay, mRNA levels were measured by reverse transcription-quantitative polymerase chain reaction and protein expression was assessed using western blot analysis. The current study demonstrated that exposure to high concentrations of CORT induced cytotoxicity and downregulated the Sonic hedgehog pathway (Shh) in PC12 cells. These effects were attenuated by EGCG. However, the EGCG-mediated neuroprotective effects, as well as upregulation of the Shh pathway were all attenuated by the Shh signaling inhibitor cyclopamine. These results indicate that EGCG protects PC12 cells from CORT-induced neurotoxicity via activation of the Shh signaling pathway. https://greenmedinfo.com/article/egcg-protects-pc12-cells-corticosterone-induced-neurotoxicity#comments EGCG (Epigallocatechin gallate) Neuroprotective Agents EGCG (Epigallocatechin gallate) Neuroprotective Agents In Vitro Study Wed, 30 May 2018 23:02:33 +0000 greenmedinfo 165015 at https://greenmedinfo.com Epigallocatechin-3-gallate regulates anti-inflammatory action in lipopolysaccharide-stimulated human intestinal epithelial cells. https://greenmedinfo.com/article/epigallocatechin-3-gallate-regulates-anti-inflammatory-action-lipopolysacchari n/a PMID:  Cell Physiol Biochem. 2018 ;46(5):2072-2081. Epub 2018 Apr 28. PMID: 29723847 Abstract Title:  Epigallocatechin-3-Gallate Regulates Anti-Inflammatory Action Through 67-kDa Laminin Receptor-Mediated Tollip Signaling Induction in Lipopolysaccharide-Stimulated Human Intestinal Epithelial Cells. Abstract:  BACKGROUND/AIMS: Inflammatory bowel disease (IBD) is a condition that involves chronic inflammation in all or part of the digestive tract. Often painful and debilitating, IBD can lead to life-threatening complications and increase the risk for colon cancer. In this study, we investigated the epigallocatechin-3-gallate (EGCG) mediated anti-inflammation response in lipopolysaccharide (LPS)-stimulated human colorectal cells through the negative regulator of Toll-like receptor (TLR) signaling. METHODS: human intestinal epithelial cells (HT-29) were used in all experiments. Cell cytotoxicity and nitric oxide (NO) were evaluated by WST-1 and the Griess reagent. Western blot analysis and ELISA were used to determine inflammatory mediators and 67-kDa laminin receptor (67LR)-mediated Tollip signaling pathways. RESULTS: Treatment of EGCG and LPS did not affect the cytotoxicity in HT-29 cells. LPS treatment dose-dependently increased the pro-inflammatory cytokine, such as interleukin (IL)-8, whereas EGCG significantly reduced the LPS-stimulated IL-8 production. Additionally, EGCG treatment markedly increased the Toll-interacting protein (Tollip) expression, which negatively regulates the TLR signaling in a dose and time-dependent manner. In particular, in the result from an RNA interference-mediated assay, our finding showed that silencing of Tollip resulted in abrogation of the inhibitory action of EGCG on LPS-induced production of pro-inflammatory mediators (inducible nitric oxide synthase-mediated NO/COX2, and IL-8) and activation of MAPKs and NF-κB signaling pathways. Interestingly, we also found that Tollip expression induced by EGCG could be modulated through 67LR expressed on the surface of HT-29 cells. CONCLUSIONS: Our novel finding indicates that 67LR and Tollip signaling activated by EGCG treatment is essential for inhibition of inflammation in human intestinal epithelial cells. https://greenmedinfo.com/article/epigallocatechin-3-gallate-regulates-anti-inflammatory-action-lipopolysacchari#comments EGCG (Epigallocatechin gallate) Inflammatory Bowel Diseases Lipopolysaccharide-Induced Toxicity Anti-Inflammatory Agents Cyclooxygenase 2 Inhibitors Interleukin-8 downregulation Anti-Inflammatory Agents EGCG (Epigallocatechin gallate) Inflammatory Bowel Diseases Lipopolysaccharide-Induced Toxicity In Vitro Study Wed, 30 May 2018 23:20:00 +0000 greenmedinfo 165017 at https://greenmedinfo.com Green Tea Compound Reduces Pancreatic Cancer Risk https://greenmedinfo.com/blog/green-tea-compound-reduces-pancreatic-cancer-risk <div class="copyright">This article is copyrighted by GreenMedInfo LLC, 2014<br/><strong><a href="/greenmedinfocom-re-post-guidelines">Visit our Re-post guidelines</a></strong></div><p class="rtecenter"><img alt="Green Tea Compound Reduces Pancreatic Cancer Risk" src="//cdn.greenmedinfo.com/sites/default/files/ckeditor/stebu/images/Green_Tea_Cup.jpg" style="width: 550px; height: 367px;" /></p> <p><strong><a href="/substance/green-tea">Green tea</a></strong> may well be the healthiest drink on the planet.&nbsp; For many, the tea and its extracts hold great promise as potential treatments for <strong><a href="/disease/cancers-all">cancer</a></strong>. But scientists had never identified how green tea helps reduce the risk of cancer.</p> <p>Now researchers from Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center have figured out how green tea works.&nbsp;</p> <p>In a study published online by the journal Metabolomics<a href="#_edn1" name="_ednref1" title="">[i]</a> they explain how an active constituent in green tea changes the metabolism of cancer cells.&nbsp;</p><p><a href="https://greenmedinfo.com/blog/green-tea-compound-reduces-pancreatic-cancer-risk" target="_blank">read more</a></p> https://greenmedinfo.com/blog/green-tea-compound-reduces-pancreatic-cancer-risk#comments Apples Blackberry Cancer Chocolate EGCG (Epigallocatechin gallate) Green Tea Onion Pancreatic Cancer Peach Plum Raspberry Red Wine Strawberry apples blackberry Cancer chocolate EGCG (Epigallocatechin gallate) GREEN TEA Pancreatic Cancer Red Wine Mon, 28 Jul 2014 15:24:56 +0000 mmking 113057 at https://greenmedinfo.com Inhibitory effect of the green tea molecule EGCG against dengue virus infection. https://greenmedinfo.com/article/inhibitory-effect-green-tea-molecule-egcg-against-dengue-virus-infection n/a PMID:  Arch Virol. 2018 Feb 10. Epub 2018 Feb 10. PMID: 29429035 Abstract Title:  Inhibitory effect of the green tea molecule EGCG against dengue virus infection. Abstract:  Dengue virus (DENV) infection is a major public health problem worldwide; however, specific antiviral drugs against it are not available. Hence, identifying effective antiviral agents for the prevention of DENV infection is important. In this study, we showed that the reportedly highly biologically active green-tea component epigallocatechin gallate (EGCG) inhibited dengue virus infection regardless of infecting serotype, but no or minimal inhibition was observed with other flaviviruses, including Japanese encephalitis virus, yellow fever virus, and Zika virus. EGCG exerted its antiviral effect mainly at the early stage of infection, probably by interacting directly with virions to prevent virus infection. Our results suggest that EGCG specifically targets DENV and might be used as a lead structure to develop an antiviral drug for use against the virus. https://greenmedinfo.com/article/inhibitory-effect-green-tea-molecule-egcg-against-dengue-virus-infection#comments Dengue Fever EGCG (Epigallocatechin gallate) Antiviral Agents Antiviral Agents Dengue Fever EGCG (Epigallocatechin gallate) In Vitro Study Fri, 16 Feb 2018 00:21:17 +0000 greenmedinfo 159911 at https://greenmedinfo.com Studies also show that catechins may prevent the formation of amyloid-β plaques and enhance cognitive functions. https://greenmedinfo.com/article/studies-also-show-catechins-may-prevent-formation-amyloid-plaques-and-enhance- n/a PMID:  J Alzheimers Dis. 2017 May 30. Epub 2017 May 30. PMID: 28582855 Abstract Title:  Diabetes and Alzheimer&#039;s Disease: Can Tea Phytochemicals Play a Role in Prevention? Abstract:  Dementia and diabetes mellitus are prevalent disorders in the elderly population. While recognized as two distinct diseases, diabetes has more recently recognized as a significant contributor to risk for developing dementia, and some studies make reference to type 3 diabetes, a condition resulting from insulin resistance in the brain. Alzheimer&#039;s disease, the most common form of dementia, and diabetes, interestingly, share underlying pathological processes, commonality in risk factors, and, importantly, pathways for intervention. Tea has been suggested to possess potent antioxidant properties rich in phytochemicals including, flavonoids, tannins, caffeine, polyphenols, boheic acid, theophylline, theobromine, anthocyanins, gallic acid, and finally epigallocatechin-3-gallate, considered the most potent active ingredient. Flavonoid phytochemicals, known as catechins, within tea offer potential benefits for reducing the risk of diabetes and Alzheimer&#039;s disease by targeting common risk factors, including obesity, hyperlipidemia, hypertension, cardiovascular disease, and stroke. Studies also show that catechins may prevent the formation of amyloid-β plaques and enhance cognitive functions, and thus may be useful in treating patients who have Alzheimer&#039;s disease or dementia. Furthermore, other phytochemicals found within tea offer important antioxidant properties along with innate properties capable of modulating intracellular neuronal signaltransduction pathways and mitochondrial function. https://greenmedinfo.com/article/studies-also-show-catechins-may-prevent-formation-amyloid-plaques-and-enhance-#comments Alzheimer's Disease Anthocyanins Caffeine Catechin Diabetic Complications EGCG (Epigallocatechin gallate) Flavonoids Gallic Acid Polyphenols Theophylline Antioxidants Neuroprotective Agents Alzheimer's disease Anthocyanins Antioxidants caffeine Catechin Diabetic Complications EGCG (Epigallocatechin gallate) Flavonoids Gallic Acid Neuroprotective Agents POLYPHENOLS Theophylline Review Wed, 07 Jun 2017 18:21:14 +0000 greenmedinfo 148778 at https://greenmedinfo.com These results indicated that miRNA-150-5p might be involved in the antihypertensive effect of EGCG. https://greenmedinfo.com/article/these-results-indicated-mirna-150-5p-might-be-involved-antihypertensive-effect n/a PMID:  Life Sci. 2018 Jun 15 ;203:193-202. Epub 2018 Apr 27. PMID: 29705350 Abstract Title:  miRNA-150-5p associate with antihypertensive effect of epigallocatechin-3-gallate revealed by aorta miRNome analysis of spontaneously hypertensive rat. Abstract:  AIMS: The antihypertensive mechanism (s) of the epigallocatechin-3-gallate (EGCG), a major effective component in green tea, might associate with microRNAs (miRNAs). Here, we aimed to investigate which microRNA in aorta of spontaneously hypertensive rats (SHRs) were modulated by administration of EGCG and its mechanism. MAIN METHODS: The pharmacokinetic behaviors of EGCG and epigallocatechin (EGC) in Sprague-Dawley rats were analyzed by HPLC and DRUG AND STATISTICS software. Blood pressure of SHRs was monitored by the tail-cuff method, the miRNomes of aorta from SHRs was analyzed with deep sequencing, and expression of hypertension-associated miRNAs with significant change and their host genes and target genes were validated by real-time PCR and Western blot. KEY FINDINGS: The plasma deposition of EGCG and EGC best fitted a mono-compartmental model with maximum plasma concentration post-dose (C, 6.65 vs 4.45 μg/ml) and the corresponding time (T, 15 vs 10 min). Systolic blood pressure (SBP) of SHRs decreased to the lowest point by 34.04 mmHg and recovered by 23.39 mmHg after 15 and 30 min of administration at dose of 300 mg/kg BW EGCG, respectively, and it decreased again at 60 min and recovered at time 2 h. Total 35 upregulated and 18downregulated miRNAs were identified compared to the control group (p &lt; .01) after EGCG administration. Expression of hypertension-associated miRNA-126a-3p and miRNA-150-5p were further validated. In turn, their host gene and target genes were up-regulated and down-regulated, respectively. SIGNIFICANCE: Our results indicated that miRNA-150-5p might be involved in the antihypertensive effect of EGCG through SP1/AT1R pathway. https://greenmedinfo.com/article/these-results-indicated-mirna-150-5p-might-be-involved-antihypertensive-effect#comments EGCG (Epigallocatechin gallate) Hypertension Antihypertensive Agents MicroRNA modulator Antihypertensive Agents EGCG (Epigallocatechin gallate) hypertension MicroRNA modulator In Vitro Study Wed, 30 May 2018 23:24:05 +0000 greenmedinfo 165020 at https://greenmedinfo.com