STAT3 Inhibitor https://greenmedinfo.com/category/keywords/STAT3%20Inhibitor en Curcumin significantly decreased glioblastoma stem cells proliferation, sphere forming ability and colony forming potential. https://greenmedinfo.com/article/curcumin-significantly-decreased-glioblastoma-stem-cells-proliferation-sphere- n/a PMID:  BMC Cancer. 2017 Feb 4 ;17(1):99. Epub 2017 Feb 4. PMID: 28160777 Abstract Title:  Curcumin decreases malignant characteristics of glioblastoma stem cells via induction of reactive oxygen species. Abstract:  BACKGROUND: Glioblastoma Multiforme (GBM) is the most common and lethal form of primary brain tumor in adults. Following standard treatment of surgery, radiation and chemotherapy, patients are expected to survive 12-14 months. Theorized cause of disease recurrence in these patients is tumor cell repopulation through the proliferation of treatment-resistant cancer stem cells. Current research has revealed curcumin, the principal ingredient in turmeric, can modulate multiple signaling pathways important for cancer stem cell self-renewal and survival. METHODS: Following resection, tumor specimens were dissociated and glioblastoma stem cells (GSCs) were propagated in neurosphere media and characterized via immunocytochemistry. Cell viability was determined with MTS assay. GSC proliferation, sphere forming and colony forming assays were conducted through standard counting methods. Reactive oxygen species (ROS) production was examined using the fluorescent molecular probe CM-H2DCFA. Effects on cell signaling pathways were elucidated by western blot. RESULTS: We evaluate the effects of curcumin on patient-derived GSC lines. We demonstrate a curcumin-induced dose-dependent decrease in GSC viability with an approximate IC50 of 25 μM. Treatment with sub-toxic levels (2.5 μM) of curcumin significantly decreased GSC proliferation, sphere forming ability and colony forming potential. Curcumin induced ROS, promoted MAPK pathway activation, downregulated STAT3 activity and IAP family members. Inhibition of ROS with the antioxidant N-acetylcysteine reversed these effects indicating a ROS dependent mechanism. CONCLUSIONS: Discoveries made in this investigation may lead to a non-toxic intervention designed to prevent recurrence in glioblastoma by targeting glioblastoma stem cells. https://greenmedinfo.com/article/curcumin-significantly-decreased-glioblastoma-stem-cells-proliferation-sphere-#comments Curcumin Glioblastoma Antiproliferative STAT3 Inhibitor Antiproliferative Cancer Stem Cells CURCUMIN Glioblastoma STAT3 Inhibitor In Vitro Study Fri, 07 Apr 2017 20:36:55 +0000 greenmedinfo 146050 at https://greenmedinfo.com Topical administration of Zerumbone accelerated corneal wound healing. https://greenmedinfo.com/article/topical-administration-zerumbone-accelerated-corneal-wound-healing n/a PMID:  Int Immunopharmacol. 2017 May 11 ;48:126-134. Epub 2017 May 11. PMID: 28501766 Abstract Title:  Therapeutic effects of zerumbone in an alkali-burned corneal wound healing model. Abstract:  Cornea is an avascular transparent tissue. Ocular trauma caused by a corneal alkali burn induces corneal neovascularization (CNV), inflammation, and fibrosis, leading to vision loss. The purpose of this study was to examine the effects of Zerumbone (ZER) on corneal wound healing caused by alkali burns in mice. CNV was induced by alkali-burn injury in BALB/C female mice. Topical ZER (three times per day, 3μl each time, at concentrations of 5, 15, and 30μM) was applied to treat alkali-burned mouse corneas for 14 consecutive days. Histopathologically, ZER treatment suppressed alkali burn-induced CNV and decreased corneal epithelial defects induced by alkali burns. Corneal tissue treated with ZER showed reduced mRNA levels of pro-angiogenic genes, including vascular endothelial growth factor, matrix metalloproteinase-2 and 9, and pro-fibrotic factors such as alpha smooth muscle actin and transforming growth factor-1 and 2. Immunohistochemical analysis demonstrated that the infiltration of F4/80and/or CCR2 positive cells was significantly decreased in ZER-treated corneas. ZER markedly inhibited the mRNA and protein levels of monocyte chemoattractant protein-1 (MCP-1) in human corneal fibroblasts and murine peritoneal macrophages. Immunoblot analysis revealed that ZER decreased the activation of signal transducer and activator of transcription 3 (STAT3), with consequent reduction of MCP-1 production by these cells. In conclusion, topical administration of ZER accelerated corneal wound healing by inhibition of STAT3 and MCP-1 production. https://greenmedinfo.com/article/topical-administration-zerumbone-accelerated-corneal-wound-healing#comments Burns Wound Healing Zerumbone STAT3 Inhibitor Burns STAT3 Inhibitor wound healing Zerumbone Animal Study Tue, 16 May 2017 23:04:29 +0000 greenmedinfo 147823 at https://greenmedinfo.com