Lung Injury: Acute https://greenmedinfo.com/category/keywords/Lung%20Injury%3A%20Acute en Nobiletin-ameliorated lipopolysaccharide-induced inflammation in acute lung injury. https://greenmedinfo.com/article/nobiletin-ameliorated-lipopolysaccharide-induced-inflammation-acute-lung-injur n/a PMID:  Inflammation. 2018 Mar 14. Epub 2018 Mar 14. PMID: 29541888 Abstract Title:  Nobiletin-Ameliorated Lipopolysaccharide-Induced Inflammation in Acute Lung Injury by Suppression of NF-κB Pathway In Vivo and Vitro. Abstract:  Nobiletin (NOB), a citrus polymethoxy flavonoid, has been reported to exhibit anti-inflammatory, anti-cancer, and anti-insulin resistance activities. Although the anti-inflammatory activity of NOB already reported, its involvement in lung protection has not been reported. Thus, this study aimed to investigate the anti-inflammatory response of NOB in lipopolysaccharide (LPS)-stimulated A549 cells and LPS-induced acute lung injury (ALI) in mice. The animals were pre-treated with NOB (5, 10, and 20 mg/kg) or DEX (5 mg/kg) at 12 and 1 h before intranasal instillation of LPS. The severity of pulmonary injury was evaluated 6 h after LPS administration. Results suggested that treatment with NOB dramatically attenuated lung histopathological changes, wet-to-dry (W/D) ratio, myeloperoxidase (MPO) activity, the numbers of inflammatory cells, and TNF-α, IL-6, and NO in BALF induced by LPS. Furthermore, NOB also significantly inhibited the expression of iNOS and the phosphorylation of NF-κBp65 and IκBα. In vitro, NOB inhibited NF-κB activation and TNF-α, IL-6 production in LPS-stimulated A549 cells. Taken together, these results indicated that NOB exhibited a protective effect on ALI, and the possible mechanism is involved in inhibiting NF-κB activation, subsequently inhibiting LPS-induced inflammatory response. https://greenmedinfo.com/article/nobiletin-ameliorated-lipopolysaccharide-induced-inflammation-acute-lung-injur#comments Lipopolysaccharide-Induced Toxicity Lung Injury: Acute Nobiletin Anti-Inflammatory Agents Interleukin-6 Downregulation NF-kappaB Inhibitor Tumor Necrosis Factor (TNF) Alpha Inhibitor Anti-Inflammatory Agents Lipopolysaccharide-Induced Toxicity Lung Injury: Acute Nobiletin In Vitro Study Mon, 09 Apr 2018 22:39:50 +0000 greenmedinfo 162384 at https://greenmedinfo.com Puerarin prevents LPS-induced acute lung injury via inhibiting inflammatory response. https://greenmedinfo.com/article/puerarin-prevents-lps-induced-acute-lung-injury-inhibiting-inflammatory-respon n/a PMID:  Microb Pathog. 2018 Mar 20 ;118:170-176. Epub 2018 Mar 20. PMID: 29571724 Abstract Title:  Puerarin prevents LPS-induced acute lung injury via inhibiting inflammatory response. Abstract:  Acute lung injury (ALI) is a critical illness syndrome with high morbidity and mortality in patients. Inflammation has been known to be involved in the development of ALI. The purpose of this study was to investigate the effect of puerarin on lipopolysaccharide (LPS)-induced ALI in mice. The pro-inflammatory cytokines TNF-α, IL-6 and IL-1β were determined by ELISA. Western blot analysis was used for detecting the expression of NF-κB, IκBα, and LXRα. And myeloperoxidase (MPO) activity, lung wet/dry (W/D) ratio, and histopathological examination were also detected in lung tissues. The results showed that puerarinsignificantly inhibited LPS-stimulated MPO activity in lung tissues. Meanwhile, puerarin attenuated lung histopathological changes and lung wet/dry (W/D) ratio. We also found that the expression of pro-inflammatory cytokines, TNF-α, IL-6 and IL-1β were inhibited by puerarin. Puerarin also inhibited LPS-induced TNF-α in RAW264.7 cells and IL-8 in A549 cells. From the results of western blotting, puerarin significantly suppressed LPS-stimulated NF-κB activation. And the expression of LXRα was dose-dependently increased by treatment of puerarin. The inhibition of puerarin on TNF-α production in RAW264.7 cells and IL-8 production in A549 cells were blocked by LXRα inhibitor geranylgeranyl pyrophosphate (GGPP). These results suggested that puerarin attenuated ALI by activating LXRα, which subsequently inhibited LPS-induced inflammatory response. https://greenmedinfo.com/article/puerarin-prevents-lps-induced-acute-lung-injury-inhibiting-inflammatory-respon#comments Lipopolysaccharide-Induced Toxicity Lung Injury: Acute Puerarin Anti-Inflammatory Agents Tumor Necrosis Factor (TNF) Alpha Inhibitor Anti-Inflammatory Agents Lipopolysaccharide-Induced Toxicity Lung Injury: Acute Puerarin Tumor Necrosis Factor (TNF) Alpha Inhibitor In Vitro Study Wed, 16 May 2018 19:21:43 +0000 greenmedinfo 164305 at https://greenmedinfo.com