Anti-Allergic Agents https://greenmedinfo.com/category/keywords/Anti-Allergic%20Agents en Adlay bran extract can reduce an allergic reaction by balancing Th1/Th2 immune responses. https://greenmedinfo.com/article/adlay-bran-extract-can-reduce-allergic-reaction-balancing-th1th2-immune-respon n/a PMID:  Food Chem Toxicol. 2012 Oct ;50(10):3808-13. Epub 2012 Jul 15. PMID: 22809477 Abstract Title:  Allergic immune-regulatory effects of adlay bran on an OVA-immunized mice allergic model. Abstract:  Allergy is an inflammation associated with an elevated T helper (Th) 2 lymphocyte responses to allergens and elevated serum IgE levels and cytokines. In one of our previous studies using a cell model, various flavonoids were found to be involved the anti-inflammatory effects of adlay bran. The present study investigated the effect of the ethyl-acetate fraction of ethanolic extract of adlay bran (ABE-EtOAc) in an ovalbumin (OVA)-immunized murine model. Six-week-old female BALB/c mice underwent OVA sensitization and were used as an allergy model. An orogastric gavage was used to force feed these mice with 240 mg/kg ABE-EtOAc from their sixth week through their twelfth week. Immune reactions were determined by measuring changes in Th2-type cytokine (IL-4 and IL-5) levels and production of antibodies. ABE-EtOAc was found capable of regulating the Th1/Th2 immune reaction through its regulation of IL-2 and IL-4. It also significantly reduced the production of anti-OVA IgE antibodies (10%), increased the secretion of IFN-γ and decreased the secretion of IL-6 (38%). These results suggest that adlay bran extract can reduce an allergic reaction by balancing Th1/Th2 immune responses and that it might be used in the treatment of this condition. https://greenmedinfo.com/article/adlay-bran-extract-can-reduce-allergic-reaction-balancing-th1th2-immune-respon#comments Adlay Allergies Anti-Allergic Agents Immunomodulatory Adlay allergies Anti-Allergic Agents Immunomodulatory Plant Extracts Animal Study Sat, 21 Apr 2018 00:00:27 +0000 greenmedinfo 163103 at https://greenmedinfo.com Adlay bran extract reduced the release of histamines and cytokines and suppressed the production of Akt. https://greenmedinfo.com/article/adlay-bran-extract-reduced-release-histamines-and-cytokines-and-suppressed-pro n/a PMID:  J Ethnopharmacol. 2012 May 7 ;141(1):119-27. Epub 2012 Feb 14. PMID: 22353428 Abstract Title:  Inhibitory effects of adlay bran (Coix lachryma-jobi L. var. ma-yuen Stapf) on chemical mediator release and cytokine production in rat basophilic leukemia cells. Abstract:  ETHNOPHARMACOLOGICAL RELEVANCE: Adlay (Job's tears, Coix lachryma-jobi L. var. ma-yuen Stapf) has long been used in China to treat rheumatism. AIM OF THE STUDY: We investigated the anti-allergic effects of adlay bran on rat basophilic leukemia (RBL)-2H3 cells. MATERIALS AND METHODS: To evaluate the anti-allergic effects of adlay bran, the release of histamines and cytokines were measured using ELISA. To explore the mechanism of these effects, the protein expression levels were determined using western blotting. RESULTS: A 40.8μg/mL concentration of the ethyl acetate fraction of the ethanolic extracts of adlay bran (ABE-EtOAc) effectively inhibited mast cell degranulation. The 40-100% EtOAc/Hex subfractions of ABE-EtOAc inhibited histamine release with an IC(50) of 71-87μg/mL. Moreover, the ABE-EtOAc subfractions suppressed the secretion of interleukin (IL)-4, IL-6 and tumor necrosis factor-α in the RBL-2H3 cells, indicating that adlay bran can inhibit cytokine secretion in the late phase of the allergic reaction. In addition, adlay bran reduced the intracellular production of reactive oxygen species, inhibited the phosphorylation of Akt and decreased the expression of protein kinase C. Furthermore, six phenolic acids and one flavone were isolated. Of these compounds, luteolin showed the most potent inhibitory activity (IC(50)=1.5μg/mL). CONCLUSION: Adlay bran extract reduced the release of histamines and cytokines and suppressed the production of Akt. These combined effects influenced the signal transduction in RBL-2H3 cells, thereby revealing the mechanisms of the anti-allergic effects of adlay. https://greenmedinfo.com/article/adlay-bran-extract-reduced-release-histamines-and-cytokines-and-suppressed-pro#comments Adlay Leukemia Anti-Allergic Agents Interleukin-4 downregulation Interleukin-6 Downregulation Protein Kinase Inhibitors Tumor Necrosis Factor (TNF) Alpha Inhibitor Adlay Anti-Allergic Agents Interleukin-4 downregulation leukemia Phytotherapy Plant Extracts Animal Study Sat, 21 Apr 2018 00:05:04 +0000 greenmedinfo 163104 at https://greenmedinfo.com These results suggest that ethanolic extracts of adlay had an inhibitory effect on allergic response. https://greenmedinfo.com/article/these-results-suggest-ethanolic-extracts-adlay-had-inhibitory-effect-allergic- n/a PMID:  J Agric Food Chem. 2010 Feb 24 ;58(4):2596-601. PMID: 20102206 Abstract Title:  Mast cell-dependent allergic responses are inhibited by ethanolic extract of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) testa. Abstract:  Allergy is an immune dysfunction caused by degranulation from mast cells in the early phase and cytokine secretion in the late phase of the cell. The purpose of this study was to investigate the effects of adlay (Job's tears, Coix lachryma-jobi L. var. ma-yuen Stapf) testa against beta-hexosaminidase release as a marker of degranulation in rat basophilic leukemia (RBL)-2H3 cells. The ethyl acetate fraction from ethanolic extracts of adlay testa (ATE-EtOAc) exhibited potent inhibitory activity that suppressed degranulation from RBL-2H3 cells stimulated by 1 microM A23187. The 20%-80% EtOAc/Hex subfractions of ATE-EtOAc significantly inhibited histamine release with a IC(50) of 75-100 microg/mL. In addition, the ATE-EtOAc subfractions suppressed interleukin (IL)-4, IL-6, and tumor necrosis factor-alpha secretion in RBL-2H3 cells, indicating that adlay testa were able to inhibit cytokine secretion. In order to explore the inhibitory mechanism of adlay testa in mast cell degranulation, we examined the activation of intracellular signaling molecules. Adlay testa inhibited the phosphorylation ERK expression. Furthermore, the two major active compounds, 4-hydroxyacetophenone and p-coumaric acid, were isolated from the ATE-EtOAc subfractions. These results suggest that ATE had an inhibitory effect on allergic response via the ERK signaling transduction in RBL-2H3 cells. https://greenmedinfo.com/article/these-results-suggest-ethanolic-extracts-adlay-had-inhibitory-effect-allergic-#comments Adlay Allergies Anti-Allergic Agents Anti-Inflammatory Agents Interleukin-4 downregulation Interleukin-6 Downregulation Tumor Necrosis Factor (TNF) Alpha Inhibitor Adlay allergies Anti-Allergic Agents Anti-Inflammatory Agents Plant Extracts In Vitro Study Sat, 21 Apr 2018 01:32:39 +0000 greenmedinfo 163124 at https://greenmedinfo.com