50-Hz magnetic field affects iron homeostasis in vitro. - GreenMedInfo Summary
50-Hz magnetic field impairs the expression of iron-related genes in the in vitro SOD1model of amyotrophic lateral sclerosis.
Int J Radiat Biol. 2019 03 ;95(3):368-377. Epub 2019 Jan 4. PMID: 30513241
PURPOSE: We characterized the response to the extremely low frequency magnetic field (ELF-MF) in an in vitro model of familial Amyotrophic Lateral Sclerosis (fALS), carrying two mutant variants of the superoxide dismutase 1 (SOD1) gene.
MATERIALS AND METHODS: SH-SY5Y human neuroblastoma cells, stably over-expressing the wild type, the G93A or the H46R mutant SOD1 cDNA, were exposed to either the ELF-MF (50 Hz, 1 mT) or the sham control field, up to 72 h. Analysis of (i) viability, proliferation and apoptosis, (ii) reactive oxygen species generation, and (iii) assessment of the iron metabolism, were carried out in all clones in response to the MF exposure.
RESULTS: We report that 50-Hz MF exposure induces: (i) no change in proliferation and viability; (ii) no modulation of the intracellular superoxide and HOlevels; (iii) a significant deregulation in the expression of iron-related genes IRP1, MFRN1 and TfR1, this evidence being exclusive for the SOD1clone and associated with a slight (p = .0512) difference in the total iron content.
CONCLUSIONS: 50-Hz MF affects iron homeostasis in the in vitro SOD1ALS model.