-Shogaol, a dietary phenolic compound, induces oxidative stress mediated mitochondrial dependant apoptosis through activation of proapoptotic factors in Hep-2 cells.
Biomed Pharmacother. 2016 Aug ;82:226-36. Epub 2016 May 13. PMID: 27470359
Ginger (Zingiber officinale) is a well-known herb used in ethnomedicine. -shogaol, a phenolic nature is a major constituent of ginger. In this study, we investigated the anticancer activity of -shogaol in Laryngeal cancer (Hep-2) cells. We demonstrated the effects of -shogaol on the cell growth and apoptosis in Hep-2 cells were analyzed by the generation of reactive oxygen species (ROS), the level of mitochondrial membrane potential (ΔYm), DNA damage and apoptotic morphological changes were analyzed by AO/EtBr, AO and Hoechst staining. Further, apoptotic protein expressions were analyzed by western blot analysis. Our results indicated that -shogaol induces apoptosis as evidenced by loss of cell viability, enhanced ROS, lipidperoxidation results in altered mitochondrial membrane potential, increased DNA damage in Hep-2 cells. Further, the prooxidant role of -shogaol inhibit Bcl-2 expression with the simultaneous up-regulation of Bax, Cytochrome c, Caspase-9 and -3 protein expressions were observed in Hep-2 cells. Thus, -shogaol induces apoptosis in Hep-2 cells through inducing oxidative damage and modulate apoptotic marker expressions. Therefore, -shogaol might be used as a therapeutic agent for the treatment of laryngeal cancer.