Active compounds from Oxheart cabbage seed extract decreased cell viability in A549 cells. - GreenMedInfo Summary
Inhibition of Growth and Induction of Apoptosis in A549 Cells by Compounds from Oxheart Cabbage Extract.
J Sci Food Agric. 2015 Dec 18. Epub 2015 Dec 18. PMID: 26679410
BACKGROUND: Oxheart cabbage (Brassica oleracea var. capitata) is a member of the Brassica genus. Although some studies on the anticancer effects of extracts from Oxheart cabbage have been reported, comprehensive information on the bioactive fractions and components from Oxheart cabbage extracts is still lacking. In this study, we isolated and identified the bioactive fractions and components from Oxheart cabbage seeds using activity-guided isolation methods.
RESULTS: The cytotoxicity and apoptotic effects of fraction II, fraction III, iberverin, sulforaphane and iberin from Oxheart cabbage seed extract were investigated. The results showed that all five components had inhibitory effects on the in-vitro growth of A549 cells which were dose-dependent. These compounds also changed the morphology of A549 cells, and their inhibitory activity on A549 cells was as follows: sulforaphane>iberin>iberverin>Fraction III>Fraction II. The IC50 values were 3.53±0.63, 4.93±1.02, 7.07±0.51, 15.56±0.24, and 27.32±0.63 µg/mL, respectively. Fraction II, fraction III, iberverin, sulforaphane and iberin induced cell apoptosis by increasing early apoptosis and late apoptosis/necrosis, and activation of caspase-3, -8 and -9.
CONCLUSION: These results indicated that the decrease in A549 cell viability by active compounds from Oxheart cabbage seed extract was due to the induction of apoptosis.