The Gastrprotective Effect of N- acetylcysteine and Genistein in Indomethacin-Induced Gastric Injury in Rats.
Can J Physiol Pharmacol. 2018 Jul 16. Epub 2018 Jul 16. PMID: 30011378
Islam Ibrahim Hegab
The protective effect of N- acetylcysteine (NAC) and genistein (GEN) on an experimental model of indomethacin (IND) - induced gastric injury was investigated. 50 male rats were divided into 5 groups: control (I), IND (II), NAC pretreated (III), GEN Pretreated (IV), NAC+GEN pretreated (V). Rats were orally administered NAC (500 mg/kg), GEN (10 mg/kg) or both once daily for 7 days before the induction of the gastric injury by IND (50 mg/kg). Stomach was removed for biochemical analysis and histopathological examinations. Pretreatment with either NAC or GEN or both significantly improved ulcer indices, increased nitric oxide (NO) level and superoxide dismutase (SOD) activity , while they significantly decreased malondialdehyde (MDA), tumor necrosis factorα (TNF α) levels and myeloperoxidase (MPO) activity, along with down-regulation of MMP-9 gene expression in pretreated groups compared to IND group. NAC alone ameliorated IND-induced apoptosis, while GEN only significantly increased PGE2 level. Further, co-administration of both recorded a bettersignificant gastroprotective effect rather than their single usage. Conclusively, concomitant administration of NAC and GEN have an additive gastroprotective effect in IND- induced gastric injury which may be through interaction of their potential cytoprotective, antioxidant, anti-inflammatory and anti-apoptotic mechanisms together with regulation of MMP-9 expression.