Abstract Title:

[All-trans retinoic acid improves iodine uptake of thyroid cancer cells via repressing transcriptional activity ofβ-catenin].

Abstract Source:

Zhonghua Yi Xue Za Zhi. 2016 Feb 23 ;96(7):553-558. PMID: 26902198

Abstract Author(s):

L Lan, W Deng, H L Chen, L L Huo, L L Deng, G Y Zhang, Y Luo

Article Affiliation:

L Lan

Abstract:

Objective: To determine whether all-trans retinoic acid (ATRA) could improve iodine uptake via repressing transcriptional activity ofβ-catenin in thyroid cancer cells. Methods: Three kinds of treatment models were firstly established with alcohol, ATRA, and transfection of β-catenin shRNA in undifferentiated human thyroid cancer cell line-SW1736.Then the expressions of sodium iodide symporter (NIS), β-catenin and its regulating factors, epithelial-mensechymal transition (EMT)-phenotype, invasion and metastasis associated proteins were further measured in above three cell models.After that, the influence of ATRA on the functional expression of NIS, iodine uptake potency, tumor growth curve and treatment effect inducing byradioactive iodine was comparatively analyzed in vitro and in vivo trials. Results: After treated with ATRA, transcriptional activity of β-catenin decreased by downregulating phosphorylation of β-catenin Ser45、Y654 and GSK-3β Ser9.Additionally, ATRA effectively upregulated the protein level ofNIS, and reversed EMT phenotype in alcohol treated cells, with absence in epithelial expression of E-cadherin and cytokeratin 18, as well as abnormal expression of Vimentin, urinary plasminogen activator (uPA), uPAR and Fibronectin.Compared with alcohol-treated group, both in vitro proliferation and invasion potential of ATRA treated cells markedly decreased (all P<0.05), and iodine uptake in vitro increased about 3.5-folds (P=0.007). In ATRA-treated animal model, tumor growth potential and tumor mass were significantly inhibited by radio-iodine ((131)I) treatment (all P<0.05). Conclusions: ATRA can increase functional expression of NIS via downregulating transcriptional activity ofβ-catenin and promote isotope sensitivity to radio-iodine in human undifferentiated thyroid cancer.

Study Type : In Vitro Study

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2020 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.