Allicin inhibits proliferation and promotes apoptosis of human epidural scar fibroblasts.
World Neurosurg. 2020 Jan 14. Epub 2020 Jan 14. PMID: 31953094
BACKGROUND: Allicin can suppress liver and cardiac fibrosis, thus we hypothesize that it may prevent scar tissue from extensive epidural fibrosis after laminectomy.
METHODS: Human epidural scar fibroblasts were isolated from surgical specimen, and treated by allicin at a gradient of concentrations. The morphology, viability, migration rate, cell cycle and apoptosis rate were measured by fluorescence microscope, CCK-8 assay, scratch assay and flow cytometry. Western Blot was used to measure the expression level of proliferation-related proteins.
RESULTS: After treated by allicin, cell viability (p=0.042*) and migration rate (p=0.010* in scratch assay, and p=0.025* in Transwell assay) decreased significantly in a dose-dependent manner. The percentage of G1 phase cells significantly decreased (p=0.017*), while that of S phase (p=0.096*) and G2 (p=0.038*) phase cells significantly increased in a dose-dependent manner. Similarly, the percentage of apoptotic cells increased significantly in a dose-dependent manner (p=0.036*). Comparing with control group, the expression level of PCNA protein (p=0.081) and Bcl-2 protein (p=0.029*) significantly decreased, while the Bax protein level significantly increased (p=0.017*).
CONCLUSION: Allicin can suppress human epidural scar fibroblasts migration, induce cell apoptosis, and block cell proliferation at S phase and G2 phase.