Alpha lipoic acid alleviates ferroptosis in the MPP + -induced PC12 cells. - GreenMedInfo Summary
Alpha lipoic acid alleviates ferroptosis in the MPP-induced PC12 cells via activating the PI3K/Akt/Nrf2 pathway.
Cell Biol Int. 2020 Nov 26. Epub 2020 Nov 26. PMID: 33241887
Lin Liu
Parkinson's disease (PD) is a typical neurodegenerative disease. Alpha lipoic acid (α-LA) can reduce the incidence of neuropathy. The present study explored the role and mechanism of α-LA in 1-methyl-4-phenylpyridinium (MPP)-induced cell model of PD. The PD model was induced via treating PC12 cells with MPPat different concentrations. MPPandα-LA effects on PC12 cells were assessed from cell viability and ferroptosis. Cell viability was detected using CCK-8 assay. MDA, 4-HNE, iron, ROS and GSH concentrations, and ferroptosis-related protein SLC7A11 and GPx4 expressions were used for ferroptosis evaluation. p-PI3K, p-Akt and Nrf2 protein levels were detected. The PI3K/Akt/Nrf2 pathway inhibitors were applied to verify the role of the PI3K/Akt/Nrf2 pathway in α-LA protection against MPP-induced decreased cell viability and ferroptosis. MPPreduced cell viability and induced ferroptosis as presented by increased MDA, 4-HNE, iron and ROS concentrations, reduced levels of GSH and ferroptosis marker proteins (SLC7A11 and GPx4).α-LA attenuated MPP-induced cell viability decline and ferroptosis. The PI3K/Akt/Nrf2 pathway was activated afterα-LA treatment. Inhibiting the PI3K/Akt/Nrf2 pathway weakened the protection of α-LA against MPPtreatment. We highlighted thatα-LA alleviated MPP-induced cell viability decrease and ferroptosis in PC12 cells via activating the PI3K/Akt/Nrf2 pathway. This article is protected by copyright. All rights reserved.