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Abstract Title:

Anticancer effect of Amygdalin (vitamin B17) on hepatocellular carcinoma cell line(HepG2) in the presence and absence of Zinc.

Abstract Source:

Anticancer Agents Med Chem. 2020 Jan 19. Epub 2020 Jan 19. PMID: 31958042

Abstract Author(s):

Mohamed A El-Desouky, Abdelgawad A Fahmi, Ibrahim Y Abdelkader, Karima M Nasraldin

Article Affiliation:

Mohamed A El-Desouky

Abstract:

BACKGROUND: Amygdalin (Vitamin B-17) is a naturally occurring vitamin found in the seeds of the fruits of Prunus Rosacea family including apricot, bitter almond, cherry, and peach.

OBJECTIVE: The purpose of this study was to examine the effect of amygdalin with and without zinc on hepatocellular carcinoma (HepG2) cell line.

METHODS: MTT assay was used to evaluate the cytotoxicity of amygdalin without zinc, amygdalin + 20µmol zinc, and amygdalin + 800µmol zinc on HepG2 cell lines. The cell cycle distribution assay was determined by flow cytometry. Apoptosis was confirmed by Annexin V-FITC/PI staining assay. Moreover, the pathway of apoptosis was determined by the percentage of change in the mean levels of P53, Bcl2, Bax, cytochrome c, and caspase-3.

RESULTS: Amygdalin without zinc showed a strong anti-HepG2 activity. Furthermore, HepG2 cell lines treatment with amygdalin + 20µmol zinc and amygdalin + 800µmol zinc showed a highly significant apoptotic effect than the effect of amygdalin without zinc. Amygdalin treatment induced the cell cycle arrest at G2/M and increased the levels of P53, Bax, cytochrome c, and caspase-3 significantly, while it decreased the level ofanti-apoptotic Bcl2.

CONCLUSION: Amygdalin is a natural anti-cancer agent which can be used for the treatment of hepatocellular carcinoma. It promotes apoptosis via the intrinsic cell death pathway (the mitochondria-initiated pathway) and cell cycle arrest at G/M. The potency of amygdalin in HepG2 treatment increased significantly by the addition of zinc.

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