Analgesic and Antipyretic Activities of Ethyl Acetate Fraction Tablet ofin Animal Models.
Evid Based Complement Alternat Med. 2021 ;2021:8848797. Epub 2021 Mar 8. PMID: 33747115
Objectives: To determine the analgesic and antipyretic activities of a tablet derived fromethyl acetate fraction (AS201-01) in animal models.
Methods: The tablet derived from AS201-01 contains an equivalent of 35 mg andrographolide per tablet. Analgesic activity was determined using an acetic acid-induced writhing test on adult male mice. A writhe was recorded by a stopwatch and was defined as the stretching of the abdomen and/or stretching of at least one hind limb. For the determination of antipyretic activity, pyrexia was induced by subcutaneous injection of 15% w/v Brewer's yeast into adult male rats. Rectal temperature was monitored at 1, 2, 3, and 4 hours after treatment.
Results: The results showed that the AS201-01 tablet had analgesic and antipyretic activity. In the acetic acid-induced writhing model, AS201-01 tablet exhibited significant analgesic effect with a 66.73% reduction in writhing response at a dose of 50 mg andrographolide/kg body weight compared to the negative control group. The tablet also showed a significant antipyretic effect. The maximum antipyretic effect was observed after the third hour of administration of the AS201-01 tablet at a dose of 100 mg andrographolide/kg body weight.
Conclusion: Tablet ofethyl acetate fraction (AS201-01) exhibited analgesic and antipyretic activities.