extract inhibit growth, biofilm formation in multidrug resistant strains of.
J Tradit Complement Med. 2020 Nov ;10(6):599-604. Epub 2019 Oct 8. PMID: 33134137
Saroj Kumar Sah
Background and aim: (Kalmegh), a valuable ancient medicinal herb is used in the treatment of several diseases in most Asian countries including India.is an opportunistic pathogen causing nosocomial infections in human. We have investigated the antimicrobial susceptibility and the presence of AmpC gene in.strain isolated from the sputum of the patient.
Experimental procedure: Antibiotic susceptibility test and phenotypic detection of AmpC/ESBL beta-lactamase were performed by combined disc diffusion test. The CEA ofwas analyzed for its antibacterial potential against susceptible and resistant strains ofthrough the broth microdilution method. Molecular detection of AmpC gene was carried by polymerase chain reaction (PCR).
Results: Antibiotic susceptibility test displayed that the clinical isolate ofwere resistant towards cephalosporins, quinolone and monobactam but susceptible to carbapenems. Combined disk diffusion demonstrated AmpC/ESBLbeta-lactamase. 250 μg/ml of CEA extract confirmed the inhibition of bacterial growth and biofilm formation compared to the antibiotic. CEA treateddisplayed a reduction of AmpC by polymerase chain reaction.
Conclusion: The present study illustrates that CEA extract ofdemonstrated potentiality to controlgrowth and biofilm formation. CEA was able to suppress the expression of gene encoding AmpC. This study proves to be an economical approach to control the growth ofwhich causes serious infections.