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Abstract Title:

Anthocyanins and phenolic acids from a wild blueberry (Vaccinium angustifolium) powder counteract lipid accumulation in THP-1-derived macrophages.

Abstract Source:

Eur J Nutr. 2016 Feb ;55(1):171-82. Epub 2015 Jan 17. PMID: 25595100

Abstract Author(s):

Cristian Del Bo', Yi Cao, Martin Roursgaard, Patrizia Riso, Marisa Porrini, Steffen Loft, Peter Møller

Article Affiliation:

Cristian Del Bo'

Abstract:

PURPOSE: Blueberries are a rich source of anthocyanins (ACNs) and phenolic acids (PA), which are hypothesized to protect against development of atherosclerosis. The present study examined the effect of an ACN- and PA-rich fractions, obtained from a wild blueberry powder, on the capacity to counteract lipid accumulation in macrophages derived from monocytic THP-1 cells. In addition, we tested the capacity of pure ACNs and their metabolites to alter lipid accumulation.

METHODS: THP-1-derived macrophages were incubated with fatty acids (500μM oleic/palmitic acid, 2:1 ratio) and different concentrations (from 0.05 to 10 μg mL(-1)) of ACN- and PA-rich fractions, pure ACN standards (malvidin, delphinidin and cyanidin 3-glucoside), and metabolites (syringic, gallic and protocatechuic acids). Lipid accumulation was quantified with the fluorescent dye Nile red.

RESULTS: Lipid accumulation was reduced at all concentrations of the ACN-rich fraction tested with a maximum reduction at 10μg mL(-1) (-27.4%; p<0.0001). The PA-rich fraction significantly reduced the lipid accumulation only at the low concentrations from 0.05µg mL(-1) to 0.3 µg mL(-1), with respect to the control with fatty acids. Supplementation with pure ACN compounds (malvidin and delphinidin-3-glucoside and its metabolic products (syringic and gallic acid)) reduced lipid accumulation especially at the low concentrations, while no significant effect was observed after cyanidin-3-glucoside and protocatechuic acid supplementation.

CONCLUSIONS: The results demonstrated a potential role of both the ACN- and PA-rich fractions and single compounds in the lipid accumulation also at concentrations close to that achievable in vivo.

Study Type : Human In Vitro

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Sayer Ji
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