Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

n/a
Abstract Title:

Anti-diabetic phenolic compounds of black carrot (Daucus carota subspecies sativus var. atrorubens Alef.) inhibit enzymes of glucose metabolism: An in silico and in vitro validation.

Abstract Source:

Med Chem. 2018 Feb 28. Epub 2018 Feb 28. PMID: 29493459

Abstract Author(s):

Suhas Gorakh Karkute, Tanmay Kumar Koley, Bijen Kumar Yengkhom, Ajay Tripathi, Shivani Srivastava, Arti Maurya, Bijendra Singh

Article Affiliation:

Suhas Gorakh Karkute

Abstract:

BACKGROUND: Black carrot is known to be effective against Type 2 diabetes. The phenolic compounds present in black carrot are responsible for this property, but limited information was available about the mechanism of action and target enzymes.

OBJECTIVE: The present study aims at understanding molecular interactions of phenolic compounds of black carrot with enzymes involved in glucose metabolism in human to identify the potential inhibitor that can be used as candidate drug molecule to control diabetes.

METHOD: In vitro assay for inhibition ofα-amylase, α-glucosidase and DPP-IV was carried out using black carrot purified extract and the standard inhibitor acarbose and vildagliptin recpectively. The inhibition activity of selected phenolic compounds was also studied by in silico docking with all these three enzymes for the proper understanding of interactions. Encapsulation of purified black carrot extract was also carried out.

RESULTS: In in vitro IC50 value of purified extract was found better than standard inhibitor acarbose forα-amylase and α-glucosidase, and vildagliptin for DPP-IV. Similarly, docking scores of few anthocyanin molecules were found higher than their respective inhibitors suggesting more effective inhibition. Among anthocyanin molecules of black carrot, cyanidin 3-xylosyl galactoside was found to be potential drug to inhibit these enzymes whereas Dipeptidyl peptidase IV was identified as the best target to control diabetes with anthocyanins of black carrot.

CONCLUSION: Anthocyanins from black carrot were found to be effective to control diabetes and very first time we propose that Cyaniding 3-xylosyl galactoside is the best potential molecule for inhibiting enzymes involved in glucose metabolism. The study also shows the encapsulation of anthocyanin compounds usingβ-cyclodextrin.

Study Type : In Vitro Study

Print Options


Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2019 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.