Anti-inflammatory Effect of Astragalin and Chlorogenic Acid on-Induced Inflammation of Sheep Endometrial Epithelium Cells.
Front Vet Sci. 2020 ;7:201. Epub 2020 Apr 30. PMID: 32426380
Endometritis is one of the main diseases which harm sheep husbandry. Astragalin and chlorogenic acid (CGA) are common active ingredients of traditional Chinese medicine (TCM) with immunoprotective, antioxidant, and anti-inflammatory properties. In the present study, sheep endometrial epithelium cells (SEECs) were successfully purified and identified, and theinflammation model of SEECs induced bywas successfully established. To explore the effect of astragalin and CGA on the inflammation induced byand its potential mechanism, six groups were set up, namely, group C, M, astragalin, CGA, BAY, and STR. Cells in group C were incubated with DMEM/F12 for 6 h, while cells in group M, astragalin, CGA, BAY, and STR were incubated with DMEM/F12, astragalin, CGA, BAY, and STR for 3 h, respectively, followed byinfection at a multiplicity of infection (MOI) of 1per cell for 3 h. Subsequently, the cells and the supernatant were collected to detect the expression of genes in the toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) pathway by ELISA, qPCR, and western blot. The results showed thatcould induce inflammation of SEECs, while astragalin and CGA could alleviate the inflammatory response induced byvia inhibiting the activation of the TLR4/NF-κB signaling pathway, which provides a theoretical and experimental foundation for preventing sheep endometritis clinically.