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Abstract Title:

Anti-proliferative profile of Anacardium occidentale polysaccharide and characterization by AFM.

Abstract Source:

Int J Biol Macromol. 2020 Aug 1 ;156:981-987. Epub 2020 Mar 16. PMID: 32194125

Abstract Author(s):

Fábio de Oliveira Silva Ribeiro, Flaviane de França Dourado, Maria Francilene Souza Silva, Lucas Moreira Brito, Claudia Pessoa, Laís Ramos Monteiro de Lima, Regina Célia Monteiro de Paula, José Roberto de Souza de Almeida Leite, Alyne Rodrigues de Araújo, Durcilene Alves da Silva

Article Affiliation:

Fábio de Oliveira Silva Ribeiro

Abstract:

This paper explores the application of cashew gum (CG) as an in vitro antiproliferative, firstly by isolating and characterizing the gum using elemental analysis, gel-permeation chromatography, nuclear magnetic resonance (NMR) and atomic force microscopy (AFM). The molar mass of isolated CG was in the order of 10-10 g/mol, with small protein traces present. Polymer characterization by NMR identified key signals correlating to galactose, glucose, rhamnose and acid-related groups. Three distinct conformational stages were observed by AFM. The impact of CG on cell morphology and viability with both tumor and non-tumor cell lines was studied by AFM and 3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay respectively. Antiproliferative activity was confirmed for HCT116 (colorectal carcinoma), B16F10 (melanoma) and HL60 (promyelocytic leukemia) cancer cell lines. A change in cell morphology was demonstrated as an increased surface roughness for HL60. Considering that a CG does not exhibit cytotoxicity to non-tumor lines, it can be seen that the CG shows selectivity for tumor cells and can be a promising biomaterial for future studies.

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