Antibacterial Photodynamic Inactivation of Fagopyrin F from Tartary Buckwheat () Flower againstand Its Biofilm.
Int J Mol Sci. 2021 Jun 8 ;22(12). Epub 2021 Jun 8. PMID: 34201389
The objective of this study was to determine reactive oxygen species (ROS) produced by fagopyrin F-rich fraction (FFF) separated from Tartary buckwheat flower extract exposed to lights and to investigate its antibacterial photodynamic inactivation (PDI) againstand its biofilm. ROS producing mechanisms involving FFF with light exposure were determined using a spectrophotometer and a fluorometer.and its biofilm inactivation after PDI treatment of FFF using blue light (BL; 450 nm) were determined by plate count method and crystal violet assay, respectively. The biofilm destruction by ROS produced from FFF after exposure to BL was visualized using confocal laser scanning microscopy (CLSM) and field emission scanning electron microscope (FE-SEM). BL among 3 light sources produced type 1 ROS the most when applying FFF as a photosensitizer. FFF exposed to BL (5 and 10 J/cm) significantly more inhibitedviability and biofilm formation than FFF without the light exposure (<0.05). In the PDI of FFF exposed to BL (10 J/cm), an apparent destruction ofand its biofilm were observed by the CLSM and FE-SEM. Antibacterial PDI effect of FFF was determined for the first time in this study.