Anticancer Activity of Methanol Extract ofLeaves and Fruits Against Proliferation, Apoptosis, and Necrosis in Huh7it Cells.
Cancer Inform. 2019 ;18:1176935119842576. Epub 2019 Apr 19. PMID: 31037025
The polyphenol plant extracts have previously been demonstrated to act as chemopreventive and anticancer agents.is a rich source of polyphenols, yet its antioxidant and anticancer activities remain poorly characterized. This study aimed to determine the anticancer activity ofleaf and fruit extracts by investigating their impact on proliferation, apoptosis, and Huh7it cell necrosis. Leaves and fruits were extracted using methanol, and the phytochemical contents were analyzed using Fourier-transform infrared spectroscopy. The antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl method. Anticancer activities were examined through MTT (3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay on Huh7it liver cancer cells. The apoptosis and necrosis conditions were examined using Annexin biomarkers V-PI and later analyzed in flow cytometry.leaves and fruit examined were found to have strong antioxidant activities with ICvalues of 7.9875µg/mL and 13.402 µg/mL, respectively. MTT assay results indicatedleaves and fruit had ICvalues>653μg/mL and>2000μg/mL, respectively. The flow cytometry analysis indicated a higher percentage of Huh7it apoptosis and necrosis in leaf extracts compared with fruit extracts. The difference in anticancer activity was attributed to differing compounds present in each extract.