The antimicrobial and antibiofilm properties of allicin againstand- A therapeutic potential for denture stomatitis.
Saudi Dent J. 2021 Feb ;33(2):105-111. Epub 2020 Feb 6. PMID: 33551624
The objective of this study is to determine the therapeutic efficacy of allicin againstand(), the common etiological agents for denture stomatitis (DS). The minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentration (MFCs) of allicin were determined by the broth microdilution method followed by checkerboard microdilution method for a synergistic interaction between allicin + nystatin and allicin + CHX. The potential of allicin to eradicateandbiofilms was assessed by treating biofilm formed on self- polymerized acrylic resin with allicin at a sub-MIC concentration for 5 min. The commercial denture cleanser (brand X) was used as a positive control. A Kruskal-Wallis test followed by the post-hoc Mann-Whitneytest was applied (SPSS 20.0), and the level of significance was set at P < 0.05. Allicin exhibited antimicrobial activity against(MIC:8µg/ml and MFC:16 µg/ml) and(MIC:8µg/ml and MBC:8 µg/ml). A synergistic interaction was observed between allicin + nystatin and allicin + CHX (FICI ≤ 0.5). Allicin exhibited significant biofilm eradication againstandbiofilms with percentages of 50.0% and 52.6%, respectively. The results of this study suggest a possible application of allicin in treatingandinfection in DS.