Antimicrobial Effects and Mechanisms of Ethanol Extracts ofSeeds Againstand Methicillin-Resistant.
Foodborne Pathog Dis. 2019 Aug ;16(8):573-580. Epub 2019 Apr 17. PMID: 30994374
seeds contain many bioactive compounds commonly used in traditional Chinese medicine. In this study, the antibacterial activity and possible mechanism ofseed ethanol extract (PCEE) against foodborne pathogens were investigated. Both methicillin-resistant(MRSA) andhad similar minimum inhibitory concentrations and minimum bactericidal concentrations of PCEE at 50 and 100 μg/mL, respectively. Furthermore, elevated OD, protein concentration, and electric conductivity indicated irreversible damage to the cytoplasmic membranes of PCEE-treated cells. Indeed, the treated cells displayed disrupted membranes, incomplete and deformed shapes, and rupture as visualized by scanning electron microscopy. Multidrug-resistance efflux pump gene expression was also analyzed by quantitative reverse transcription PCR. Although the,, andgenes ofand thegene of MRSA were upregulated, there was no significant difference that indicated an attempt by the efflux pumps to discharge PCEE. MRSAexpression andexpression were significantly downregulated ( < 0.05). A possible mechanism for PCEE may be to cause an energy depletion, either by inhibiting adenosine triphosphate binding or by disturbing the proton gradient, resulting in membrane damage.