Antioxidant activities of ethanolic and acidic ethanolic extracts of astringent persimmon in HO-stimulated Caco-2 human colonic epithelial cells.
Food Sci Biotechnol. 2017 ;26(4):1085-1091. Epub 2017 Aug 18. PMID: 30263640
The aim of this study was to investigate the protective effects of ethanol (EAP) and acidic ethanol extracts (AEAP) of astringent persimmon fruits () against hydrogen peroxide (HO)-induced oxidative stress in Caco-2 epithelial cells. Levels of reactive oxygen species (ROS) and reduced glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT), as well as protein and DNA damage were measured. Treatment with HOinduced oxidative stress in Caco-2 cells but had no effect on SOD activity. Both EAP and AEAP protected the cells against oxidative damage and exhibited similar effects on cellular ROS scavenging activity and protein carbonyl levels. However, AEAP was more potent than EAP in normalizing CAT activity and cytosolic GSH level, and in protecting against DNA damage. These results demonstrated that astringent persimmon fruit extracts were biologically active, and that the tannin-rich fraction (AEAP) possesses stronger protective activity against HO-induced oxidative stress in Caco-2 cells.