Antioxidant and Hemolysis Protective Effects of Polyphenol-Rich Extract from Mulberry Fruits.
Pharmacogn Mag. 2018 Jan-Mar;14(53):103-109. Epub 2018 Feb 20. PMID: 29576709
Palanigounder Ganeshan Ajay Krishna
Background: Mulberry fruits are a superior source of polyphenol, especially anthocyanins that contribute potentially to the beneficial effects which include reducing the risk of cardiovascular diseases and cancers with antioxidant, anti-inflammatory, and chemoprotective properties.
Objectives: In this study, purification of the polyphenol-rich extract from mulberry fruit (MPE) was purified and assessed the activities of antioxidant and hemolysis protectiveand.
Materials and Methods: Antioxidant activitieswas measured by quantifying its 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, reducing power and Fe-chelating ability. MPE was purified by high-pressure liquid chromatography (HPLC) and analyzed individual polyphenols using liquid chromatography-mass spectrometry (LC-MS)/MS.
Results: The total polyphenol content was 147.69± 0.02 mg gallic acid equivalents (GAE)/g dried weight (DW) in the extract and 403.55 ± 0.02 mg GAE/g DW in the purified extract. Further identification by HPLC-ultraviolet-visible and LC-MS/MS analysis indicated in MPE, an anthocyanin compound, cyanidin-3--glucoside. With regard toassays, MPE possessed antioxidant effect, especially in Fechelating ability with an ICvalue of 1.016 mg/mL. The protective effects on mouse red blood cell hemolysis and lipid peroxidationwere dose and time dependent.
Conclusion: It indicates that MPE could be a good candidate for future biomedical applications to promote human health with limited side effects.
SUMMARY: Mulberry fruit is an excellent source of polyphenols, in particular, anthocyanins, which has infinite health benefits. This study determined the predominant anthocyanin, cyanidin-3-glucoside, could possibly be the rationale behind the antioxidant and antihemolytic effect of MPE. Results indicate that MPE could be a good candidate for future biomedical applications to promote human health with limited side effects.MPE: Purification of the polyphenol-rich extract from mulberry fruit, LC-MS: Liquid chromatography-mass spectrometry, HPLC: High-pressure liquid chromatography, DPPH: 2,2-diphenyl-1-picrylhydrazyl scavenging activity, RBC: Red blood cell, GAE: Gallic acid equivalent, FeCl: Ferrous chloride, HO: Hydrogen peroxide, EDTA-2Na: Ethylenediaminetetraacetic acid disodium salt, PBS: Phosphate-buffered saline, TCA: Trichloroacetic acid, TBA: 2-thiobarbituric acid, FeSO: Ferrous sulphate, MDA: Malondialdehyde, V: Vitamin C, DW: Dried weight.