Antispasmodic activity of prenylated phenolic compounds from the root bark of Morus nigra. - GreenMedInfo Summary
Antispasmodic Activity of Prenylated Phenolic Compounds from the Root Bark of.
Molecules. 2019 Jul 8 ;24(13). Epub 2019 Jul 8. PMID: 31288489
Black mulberry is a widely acknowledged ancient traditional medicine. Its extract and constituents have been reported to exert various bioactivities including antimicrobial, hypotensive, analgesic etc. effects. While black mulberry preparations are also used as antispasmodic agents in folk medicine, no related studies are available on its isolated constituents. Through an extensive chromatographic purification, seven phenolic compounds were isolated from the methanol extract ofroot bark, including morusin (), kuwanon U (), kuwanon E (), moracin P (), moracin O (), albanol A (), and albanol B (). A complete NMR signal assignment of moracin P and O was achieved, and related literature errors confusing the identity of moracin derivatives are hereby clarified. Compounds,andwere identified as strong antispasmodic agents on isolated rat ileum and tracheal smooth muscles, while compound, a methoxy derivative of, was inactive. Moracin O () inhibited the ileal and tracheal smooth muscle contractions with Evalues of 85% and 302 mg, respectively. Those actions were superior as compared with papaverine. Our findings demonstrate that prenylated arylbenzofurans, geranylated flavonoids and Diels-Alder adducts fromare valuable antispasmodic agents. Compounds,andare suggested as marker compounds for quality control of antispasmodic mulberry preparations. Moracin O () is a new lead compound for related drug development initiatives.