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Abstract Title:

Apigenin Inhibits Proliferation, Migration, and the Invasion of human tongue carcinoma Tca8113 Cells through Regulating the MAPK Signaling Pathways.

Abstract Source:

Curr Mol Med. 2020 Oct 22. Epub 2020 Oct 22. PMID: 33092506

Abstract Author(s):

Yun-Ze Xuan, Li-Zhi Xu, Cheng-Ri Jin, Kang-Juan Yang

Article Affiliation:

Yun-Ze Xuan

Abstract:

OBJECTIVE: This study aimed to examine the effects of apigenin (API) on the proliferation, migration, and invasion of human tongue squamous cell carcinoma Tca8113 cells and explore its probable mechanisms.

METHODS: After treating Tca8113 cells with API, the cell proliferation, migration, and invasivecapacities were identified by te-trazolium salt colorimetry(MTT) assay, cell scratch test, and Transwell chamber test. Cellular immunofluorescence staining was used to localize mitogen-activated protein kinase 1 (MAPK1) and extracellular regulated protein kinase (ERK) 1/2 proteins. Western blot was used to detect the variations of the related protein expression levels.

RESULTS: ① Through the MTT assay, API significantly inhibited cell proliferation (P<0.01).②In the cell scratch test, the dis-tance of lateral migration after the API treatment was significantly shorter compared to the control group (P<0.01).③The inva-sion rate in the lower chamber of the Transwell chamber was lower in the API group (P<0.01).④ Cellular immunofluores-cence staining presented that the total-MEKK1 was localized in the cytoplasm, p-MEKK1 was localized in the nuclear mem-brane and cytoplasm, and p-ERK1/2 was localized in the cytoplasm and nucleus. ⑤ After API was applied to cells, the expres-sions of p-MEKK1 and p-ERK1/2proteins significantly reduced (P<0.01).

CONCLUSION: Apigenin(API) significantly inhibits the proliferation, migration, and invasion of Tca8113 cells and its mecha-nism may be associatedwith the MAPK signaling pathway.

Study Type : In Vitro Study

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